Maize nutrition quality and plant tissue analysis laboratory: laboratory protocols 2008
Biochemical and chemical analysis of seeds and green plant tissues are essential in several breeding programs including nutritional and industrial enhancement, plant physiology, and plant pathology. The objective of CIMMYT’s maize nutritional quality and plant analysis laboratory is to develop and/or adopt methodologies to support the breeding programs. Therefore, wherever possible, we seek robust, cheap, and fast methodologies that allow us to provide accurate data to breeders for further field decisions. Ninety-six well microplate readers and near infrared reflectance (NIR) are two of the options we are currently using in the establishment of the biochemical and chemical platform for analysis. All methodologies presented here have been validated either by comparison with other methods or by inter-laboratory assessments. This manual compiles all current methodologies used in the laboratory and aims to serve as a guide for other laboratories or institutions that are interested in the methodologies. For quality protein maize assurance we present the tryptophan, lysine, and protein determination protocols. For provitamin A enhancement, the high performance liquid chromatography (HPLC) carotenoid protocol is included. For micronutrient analysis we present current iron and zinc determination by inductively coupled plasma-optical emission spectroscopy (ICP-OES). Other analysis performed in our lab are soluble sugars determination, anthocyanin content, total and free phenols, ash content, oil content, starch determination and amylase/amylopectin determination.
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| Autores principales: | , , , |
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| Formato: | Libro biblioteca |
| Idioma: | English |
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CIMMYT
2009
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| Materias: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, METHODS, MOLECULAR CLONING, TRANSGENIC PLANTS, PCR, DNA HYBRIDIZATION, ZEA MAYS, MAIZE, RESEARCH PROJECTS, WHEAT, |
| Acceso en línea: | http://hdl.handle.net/10883/804 |
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dig-cimmyt-10883-8042023-04-03T20:32:21Z Maize nutrition quality and plant tissue analysis laboratory: laboratory protocols 2008 Galicia, L. Nurit, E. Rosales-Nolasco, A. Palacios-Rojas, N. AGRICULTURAL SCIENCES AND BIOTECHNOLOGY METHODS MOLECULAR CLONING TRANSGENIC PLANTS PCR DNA HYBRIDIZATION ZEA MAYS MAIZE RESEARCH PROJECTS WHEAT METHODS MOLECULAR CLONING TRANSGENIC PLANTS PCR DNA HYBRIDIZATION ZEA MAYS MAIZE RESEARCH PROJECTS WHEAT Biochemical and chemical analysis of seeds and green plant tissues are essential in several breeding programs including nutritional and industrial enhancement, plant physiology, and plant pathology. The objective of CIMMYT’s maize nutritional quality and plant analysis laboratory is to develop and/or adopt methodologies to support the breeding programs. Therefore, wherever possible, we seek robust, cheap, and fast methodologies that allow us to provide accurate data to breeders for further field decisions. Ninety-six well microplate readers and near infrared reflectance (NIR) are two of the options we are currently using in the establishment of the biochemical and chemical platform for analysis. All methodologies presented here have been validated either by comparison with other methods or by inter-laboratory assessments. This manual compiles all current methodologies used in the laboratory and aims to serve as a guide for other laboratories or institutions that are interested in the methodologies. For quality protein maize assurance we present the tryptophan, lysine, and protein determination protocols. For provitamin A enhancement, the high performance liquid chromatography (HPLC) carotenoid protocol is included. For micronutrient analysis we present current iron and zinc determination by inductively coupled plasma-optical emission spectroscopy (ICP-OES). Other analysis performed in our lab are soluble sugars determination, anthocyanin content, total and free phenols, ash content, oil content, starch determination and amylase/amylopectin determination. ii, 42 pages 2012-01-06T04:37:46Z 2012-01-06T04:37:46Z 2009 Book 978-970-648-169-6 http://hdl.handle.net/10883/804 English CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose. Open Access PDF Mexico CIMMYT |
| institution |
CIMMYT |
| collection |
DSpace |
| country |
México |
| countrycode |
MX |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-cimmyt |
| tag |
biblioteca |
| region |
America del Norte |
| libraryname |
CIMMYT Library |
| language |
English |
| topic |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY METHODS MOLECULAR CLONING TRANSGENIC PLANTS PCR DNA HYBRIDIZATION ZEA MAYS MAIZE RESEARCH PROJECTS WHEAT METHODS MOLECULAR CLONING TRANSGENIC PLANTS PCR DNA HYBRIDIZATION ZEA MAYS MAIZE RESEARCH PROJECTS WHEAT AGRICULTURAL SCIENCES AND BIOTECHNOLOGY METHODS MOLECULAR CLONING TRANSGENIC PLANTS PCR DNA HYBRIDIZATION ZEA MAYS MAIZE RESEARCH PROJECTS WHEAT METHODS MOLECULAR CLONING TRANSGENIC PLANTS PCR DNA HYBRIDIZATION ZEA MAYS MAIZE RESEARCH PROJECTS WHEAT |
| spellingShingle |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY METHODS MOLECULAR CLONING TRANSGENIC PLANTS PCR DNA HYBRIDIZATION ZEA MAYS MAIZE RESEARCH PROJECTS WHEAT METHODS MOLECULAR CLONING TRANSGENIC PLANTS PCR DNA HYBRIDIZATION ZEA MAYS MAIZE RESEARCH PROJECTS WHEAT AGRICULTURAL SCIENCES AND BIOTECHNOLOGY METHODS MOLECULAR CLONING TRANSGENIC PLANTS PCR DNA HYBRIDIZATION ZEA MAYS MAIZE RESEARCH PROJECTS WHEAT METHODS MOLECULAR CLONING TRANSGENIC PLANTS PCR DNA HYBRIDIZATION ZEA MAYS MAIZE RESEARCH PROJECTS WHEAT Galicia, L. Nurit, E. Rosales-Nolasco, A. Palacios-Rojas, N. Maize nutrition quality and plant tissue analysis laboratory: laboratory protocols 2008 |
| description |
Biochemical and chemical analysis of seeds and green plant tissues are essential in several breeding programs including nutritional and industrial enhancement, plant physiology, and plant pathology. The objective of CIMMYT’s maize nutritional quality and plant analysis laboratory is to develop and/or adopt methodologies to support the breeding programs. Therefore, wherever possible, we seek robust, cheap, and fast methodologies that allow us to provide accurate data to breeders for further field decisions. Ninety-six well microplate readers and near infrared reflectance (NIR) are two of the options we are currently using in the establishment of the biochemical and chemical platform for analysis. All methodologies presented here have been validated either by comparison with other methods or by inter-laboratory assessments. This manual compiles all current methodologies used in the laboratory and aims to serve as a guide for other laboratories or institutions that are interested in the methodologies. For quality protein maize assurance we present the tryptophan, lysine, and protein determination protocols. For provitamin A enhancement, the high performance liquid chromatography (HPLC) carotenoid protocol is included. For micronutrient analysis we present current iron and zinc determination by inductively coupled plasma-optical emission spectroscopy (ICP-OES). Other analysis performed in our lab are soluble sugars determination, anthocyanin content, total and free phenols, ash content, oil content, starch determination and amylase/amylopectin determination. |
| format |
Book |
| topic_facet |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY METHODS MOLECULAR CLONING TRANSGENIC PLANTS PCR DNA HYBRIDIZATION ZEA MAYS MAIZE RESEARCH PROJECTS WHEAT METHODS MOLECULAR CLONING TRANSGENIC PLANTS PCR DNA HYBRIDIZATION ZEA MAYS MAIZE RESEARCH PROJECTS WHEAT |
| author |
Galicia, L. Nurit, E. Rosales-Nolasco, A. Palacios-Rojas, N. |
| author_facet |
Galicia, L. Nurit, E. Rosales-Nolasco, A. Palacios-Rojas, N. |
| author_sort |
Galicia, L. |
| title |
Maize nutrition quality and plant tissue analysis laboratory: laboratory protocols 2008 |
| title_short |
Maize nutrition quality and plant tissue analysis laboratory: laboratory protocols 2008 |
| title_full |
Maize nutrition quality and plant tissue analysis laboratory: laboratory protocols 2008 |
| title_fullStr |
Maize nutrition quality and plant tissue analysis laboratory: laboratory protocols 2008 |
| title_full_unstemmed |
Maize nutrition quality and plant tissue analysis laboratory: laboratory protocols 2008 |
| title_sort |
maize nutrition quality and plant tissue analysis laboratory: laboratory protocols 2008 |
| publisher |
CIMMYT |
| publishDate |
2009 |
| url |
http://hdl.handle.net/10883/804 |
| work_keys_str_mv |
AT galicial maizenutritionqualityandplanttissueanalysislaboratorylaboratoryprotocols2008 AT nurite maizenutritionqualityandplanttissueanalysislaboratorylaboratoryprotocols2008 AT rosalesnolascoa maizenutritionqualityandplanttissueanalysislaboratorylaboratoryprotocols2008 AT palaciosrojasn maizenutritionqualityandplanttissueanalysislaboratorylaboratoryprotocols2008 |
| _version_ |
1762944399162998784 |