Genomic regions associated with resistance to three rusts in CIMMYT wheat line “Mokue#1”
Understanding the genetic basis of rust resistance in elite CIMMYT wheat germplasm enhances breeding and deployment of durable resistance globally. “Mokue#1”, released in 2023 in Pakistan as TARNAB Gandum-1, has exhibited high levels of resistance to stripe rust, leaf rust, and stem rust pathotypes present at multiple environments in Mexico and Kenya at different times. To determine the genetic basis of resistance, a F5 recombinant inbred line (RIL) mapping population consisting of 261 lines was developed and phenotyped for multiple years at field sites in Mexico and Kenya under the conditions of artificially created rust epidemics. DArTSeq genotyping was performed, and a linkage map was constructed using 7892 informative polymorphic markers. Composite interval mapping identified three significant and consistent loci contributed by Mokue: QLrYr.cim-1BL and QLrYr.cim-2AS on chromosome 1BL and 2AS, respectively associated with stripe rust and leaf rust resistance, and QLrSr.cim-2DS on chromosome 2DS for leaf rust and stem rust resistance. The QTL on 1BL was confirmed to be the Lr46/Yr29 locus, whereas the QTL on 2AS represented the Yr17/Lr37 region on the 2NS/2AS translocation. The QTL on 2DS was a unique locus conferring leaf rust resistance in Mexico and stem rust resistance in Kenya. In addition to these pleiotropic loci, four minor QTLs were also identified on chromosomes 2DL and 6BS associated with stripe rust, and 3AL and 6AS for stem rust, respectively, using the Kenya disease severity data. Significant decreases in disease severities were also demonstrated due to additive effects of QTLs when present in combinations.
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2023
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Subjects: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, Genetic Analysis, Leaf Rust, GENETICS, RUSTS, QUANTITATIVE TRAIT LOCI MAPPING, DISEASE RESISTANCE, STRIPE RUST, STEM RUST, Wheat, |
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dig-cimmyt-10883-226952023-09-01T09:00:21Z Genomic regions associated with resistance to three rusts in CIMMYT wheat line “Mokue#1” Qureshi, N. Singh, R.P. Gonzalez, B.M. Velazquez-Miranda, H. Bhavani, S. AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Genetic Analysis Leaf Rust GENETICS RUSTS QUANTITATIVE TRAIT LOCI MAPPING DISEASE RESISTANCE STRIPE RUST STEM RUST Wheat Understanding the genetic basis of rust resistance in elite CIMMYT wheat germplasm enhances breeding and deployment of durable resistance globally. “Mokue#1”, released in 2023 in Pakistan as TARNAB Gandum-1, has exhibited high levels of resistance to stripe rust, leaf rust, and stem rust pathotypes present at multiple environments in Mexico and Kenya at different times. To determine the genetic basis of resistance, a F5 recombinant inbred line (RIL) mapping population consisting of 261 lines was developed and phenotyped for multiple years at field sites in Mexico and Kenya under the conditions of artificially created rust epidemics. DArTSeq genotyping was performed, and a linkage map was constructed using 7892 informative polymorphic markers. Composite interval mapping identified three significant and consistent loci contributed by Mokue: QLrYr.cim-1BL and QLrYr.cim-2AS on chromosome 1BL and 2AS, respectively associated with stripe rust and leaf rust resistance, and QLrSr.cim-2DS on chromosome 2DS for leaf rust and stem rust resistance. The QTL on 1BL was confirmed to be the Lr46/Yr29 locus, whereas the QTL on 2AS represented the Yr17/Lr37 region on the 2NS/2AS translocation. The QTL on 2DS was a unique locus conferring leaf rust resistance in Mexico and stem rust resistance in Kenya. In addition to these pleiotropic loci, four minor QTLs were also identified on chromosomes 2DL and 6BS associated with stripe rust, and 3AL and 6AS for stem rust, respectively, using the Kenya disease severity data. Significant decreases in disease severities were also demonstrated due to additive effects of QTLs when present in combinations. 2023-08-31T20:20:12Z 2023-08-31T20:20:12Z 2023 Article Published Version https://hdl.handle.net/10883/22695 10.3390/ijms241512160 English CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose Open Access Basel (Switzerland) MDPI 15 24 1661-6596 International Journal of Molecular Sciences 12160 |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Genetic Analysis Leaf Rust GENETICS RUSTS QUANTITATIVE TRAIT LOCI MAPPING DISEASE RESISTANCE STRIPE RUST STEM RUST Wheat AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Genetic Analysis Leaf Rust GENETICS RUSTS QUANTITATIVE TRAIT LOCI MAPPING DISEASE RESISTANCE STRIPE RUST STEM RUST Wheat |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Genetic Analysis Leaf Rust GENETICS RUSTS QUANTITATIVE TRAIT LOCI MAPPING DISEASE RESISTANCE STRIPE RUST STEM RUST Wheat AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Genetic Analysis Leaf Rust GENETICS RUSTS QUANTITATIVE TRAIT LOCI MAPPING DISEASE RESISTANCE STRIPE RUST STEM RUST Wheat Qureshi, N. Singh, R.P. Gonzalez, B.M. Velazquez-Miranda, H. Bhavani, S. Genomic regions associated with resistance to three rusts in CIMMYT wheat line “Mokue#1” |
description |
Understanding the genetic basis of rust resistance in elite CIMMYT wheat germplasm enhances breeding and deployment of durable resistance globally. “Mokue#1”, released in 2023 in Pakistan as TARNAB Gandum-1, has exhibited high levels of resistance to stripe rust, leaf rust, and stem rust pathotypes present at multiple environments in Mexico and Kenya at different times. To determine the genetic basis of resistance, a F5 recombinant inbred line (RIL) mapping population consisting of 261 lines was developed and phenotyped for multiple years at field sites in Mexico and Kenya under the conditions of artificially created rust epidemics. DArTSeq genotyping was performed, and a linkage map was constructed using 7892 informative polymorphic markers. Composite interval mapping identified three significant and consistent loci contributed by Mokue: QLrYr.cim-1BL and QLrYr.cim-2AS on chromosome 1BL and 2AS, respectively associated with stripe rust and leaf rust resistance, and QLrSr.cim-2DS on chromosome 2DS for leaf rust and stem rust resistance. The QTL on 1BL was confirmed to be the Lr46/Yr29 locus, whereas the QTL on 2AS represented the Yr17/Lr37 region on the 2NS/2AS translocation. The QTL on 2DS was a unique locus conferring leaf rust resistance in Mexico and stem rust resistance in Kenya. In addition to these pleiotropic loci, four minor QTLs were also identified on chromosomes 2DL and 6BS associated with stripe rust, and 3AL and 6AS for stem rust, respectively, using the Kenya disease severity data. Significant decreases in disease severities were also demonstrated due to additive effects of QTLs when present in combinations. |
format |
Article |
topic_facet |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Genetic Analysis Leaf Rust GENETICS RUSTS QUANTITATIVE TRAIT LOCI MAPPING DISEASE RESISTANCE STRIPE RUST STEM RUST Wheat |
author |
Qureshi, N. Singh, R.P. Gonzalez, B.M. Velazquez-Miranda, H. Bhavani, S. |
author_facet |
Qureshi, N. Singh, R.P. Gonzalez, B.M. Velazquez-Miranda, H. Bhavani, S. |
author_sort |
Qureshi, N. |
title |
Genomic regions associated with resistance to three rusts in CIMMYT wheat line “Mokue#1” |
title_short |
Genomic regions associated with resistance to three rusts in CIMMYT wheat line “Mokue#1” |
title_full |
Genomic regions associated with resistance to three rusts in CIMMYT wheat line “Mokue#1” |
title_fullStr |
Genomic regions associated with resistance to three rusts in CIMMYT wheat line “Mokue#1” |
title_full_unstemmed |
Genomic regions associated with resistance to three rusts in CIMMYT wheat line “Mokue#1” |
title_sort |
genomic regions associated with resistance to three rusts in cimmyt wheat line “mokue#1” |
publisher |
MDPI |
publishDate |
2023 |
url |
https://hdl.handle.net/10883/22695 |
work_keys_str_mv |
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1775949603468738560 |