Effect of F1 and F2 generations on genetic variability and working steps of doubled haploid production in maize
For doubled haploid (DH) production in maize, F1 generation has been the most frequently used for haploid induction due to facility in the process. However, using F2 generation would be a good alternative to increase genetic variability owing to the additional recombination in meiosis. Our goals were to compare the effect of F1 and F2 generations on DH production in tropical germplasm, evaluating the R1-navajo expression in seeds, the working steps of the methodology, and the genetic variability of the DH lines obtained. Sources germplasm in F1 and F2 generations were crossed with the tropicalized haploid inducer LI-ESALQ. After harvest, for both induction crosses were calculated the haploid induction rate (HIR), diploid seed rate (DSR), and inhibition seed rate (ISR) using the total number of seeds obtained. In order to study the effectiveness of the DH working steps in each generation, the percentage per se and the relative percentage were verified. In addition, SNP markers were obtained for genetic variability studies. Results showed that the values for HIR, ISR, and DSR were 1.23%, 23.48%, and 75.21% for F1 and 1.78%, 15.82%, and 82.38% for F2, respectively. The effectiveness of the DH working step showed the same percentage per se value (0.4%) for F1 and F2, while the relative percentage was 27.2% for F1 and 22.4% for F2. Estimates of population parameters in DH lines from F1 were higher than F2. Furthermore, population structure and kinship analyses showed that one additional generation was not sufficient to create new genotype subgroups. Additionally, the relative efficiency of the response to selection in the F1 was 31.88% higher than F2 due to the number of cycles that are used to obtain the DH. Our results showed that in tropical maize, the use of F1 generation is recommended due to a superior balance between time and genetic variability.
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Langue: | English |
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Public Library of Science
2019
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Sujets: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, Doubled Haploid, Tropical Germplasm, Genetic Variability, DIPLOIDY, GENETIC MARKERS, GENETIC VARIATION, GERMPLASM, MAIZE, POPULATION STRUCTURE, SINGLE NUCLEOTIDE POLYMORPHISM, PLANT BREEDING, Genetic Resources, |
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dig-cimmyt-10883-225102023-02-11T10:00:28Z Effect of F1 and F2 generations on genetic variability and working steps of doubled haploid production in maize De Oliveira Couto, E.G. Cury, M.N. Bandeira e Sousa, M. Granato, I. Vidotti, M.S. Garbuglio, D.D. Crossa, J. Burgueño, J. Fritsche-Neto, R. AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Doubled Haploid Tropical Germplasm Genetic Variability DIPLOIDY GENETIC MARKERS GENETIC VARIATION GERMPLASM MAIZE POPULATION STRUCTURE SINGLE NUCLEOTIDE POLYMORPHISM PLANT BREEDING Genetic Resources For doubled haploid (DH) production in maize, F1 generation has been the most frequently used for haploid induction due to facility in the process. However, using F2 generation would be a good alternative to increase genetic variability owing to the additional recombination in meiosis. Our goals were to compare the effect of F1 and F2 generations on DH production in tropical germplasm, evaluating the R1-navajo expression in seeds, the working steps of the methodology, and the genetic variability of the DH lines obtained. Sources germplasm in F1 and F2 generations were crossed with the tropicalized haploid inducer LI-ESALQ. After harvest, for both induction crosses were calculated the haploid induction rate (HIR), diploid seed rate (DSR), and inhibition seed rate (ISR) using the total number of seeds obtained. In order to study the effectiveness of the DH working steps in each generation, the percentage per se and the relative percentage were verified. In addition, SNP markers were obtained for genetic variability studies. Results showed that the values for HIR, ISR, and DSR were 1.23%, 23.48%, and 75.21% for F1 and 1.78%, 15.82%, and 82.38% for F2, respectively. The effectiveness of the DH working step showed the same percentage per se value (0.4%) for F1 and F2, while the relative percentage was 27.2% for F1 and 22.4% for F2. Estimates of population parameters in DH lines from F1 were higher than F2. Furthermore, population structure and kinship analyses showed that one additional generation was not sufficient to create new genotype subgroups. Additionally, the relative efficiency of the response to selection in the F1 was 31.88% higher than F2 due to the number of cycles that are used to obtain the DH. Our results showed that in tropical maize, the use of F1 generation is recommended due to a superior balance between time and genetic variability. 2023-02-10T19:08:00Z 2023-02-10T19:08:00Z 2019 Article Published Version https://hdl.handle.net/10883/22510 10.1371/journal.pone.0224631 English CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose Open Access San Francisco, CA (USA) Public Library of Science 11 14 0224631 1932-6203 PLoS ONE |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Doubled Haploid Tropical Germplasm Genetic Variability DIPLOIDY GENETIC MARKERS GENETIC VARIATION GERMPLASM MAIZE POPULATION STRUCTURE SINGLE NUCLEOTIDE POLYMORPHISM PLANT BREEDING Genetic Resources AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Doubled Haploid Tropical Germplasm Genetic Variability DIPLOIDY GENETIC MARKERS GENETIC VARIATION GERMPLASM MAIZE POPULATION STRUCTURE SINGLE NUCLEOTIDE POLYMORPHISM PLANT BREEDING Genetic Resources |
spellingShingle |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Doubled Haploid Tropical Germplasm Genetic Variability DIPLOIDY GENETIC MARKERS GENETIC VARIATION GERMPLASM MAIZE POPULATION STRUCTURE SINGLE NUCLEOTIDE POLYMORPHISM PLANT BREEDING Genetic Resources AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Doubled Haploid Tropical Germplasm Genetic Variability DIPLOIDY GENETIC MARKERS GENETIC VARIATION GERMPLASM MAIZE POPULATION STRUCTURE SINGLE NUCLEOTIDE POLYMORPHISM PLANT BREEDING Genetic Resources De Oliveira Couto, E.G. Cury, M.N. Bandeira e Sousa, M. Granato, I. Vidotti, M.S. Garbuglio, D.D. Crossa, J. Burgueño, J. Fritsche-Neto, R. Effect of F1 and F2 generations on genetic variability and working steps of doubled haploid production in maize |
description |
For doubled haploid (DH) production in maize, F1 generation has been the most frequently used for haploid induction due to facility in the process. However, using F2 generation would be a good alternative to increase genetic variability owing to the additional recombination in meiosis. Our goals were to compare the effect of F1 and F2 generations on DH production in tropical germplasm, evaluating the R1-navajo expression in seeds, the working steps of the methodology, and the genetic variability of the DH lines obtained. Sources germplasm in F1 and F2 generations were crossed with the tropicalized haploid inducer LI-ESALQ. After harvest, for both induction crosses were calculated the haploid induction rate (HIR), diploid seed rate (DSR), and inhibition seed rate (ISR) using the total number of seeds obtained. In order to study the effectiveness of the DH working steps in each generation, the percentage per se and the relative percentage were verified. In addition, SNP markers were obtained for genetic variability studies. Results showed that the values for HIR, ISR, and DSR were 1.23%, 23.48%, and 75.21% for F1 and 1.78%, 15.82%, and 82.38% for F2, respectively. The effectiveness of the DH working step showed the same percentage per se value (0.4%) for F1 and F2, while the relative percentage was 27.2% for F1 and 22.4% for F2. Estimates of population parameters in DH lines from F1 were higher than F2. Furthermore, population structure and kinship analyses showed that one additional generation was not sufficient to create new genotype subgroups. Additionally, the relative efficiency of the response to selection in the F1 was 31.88% higher than F2 due to the number of cycles that are used to obtain the DH. Our results showed that in tropical maize, the use of F1 generation is recommended due to a superior balance between time and genetic variability. |
format |
Article |
topic_facet |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Doubled Haploid Tropical Germplasm Genetic Variability DIPLOIDY GENETIC MARKERS GENETIC VARIATION GERMPLASM MAIZE POPULATION STRUCTURE SINGLE NUCLEOTIDE POLYMORPHISM PLANT BREEDING Genetic Resources |
author |
De Oliveira Couto, E.G. Cury, M.N. Bandeira e Sousa, M. Granato, I. Vidotti, M.S. Garbuglio, D.D. Crossa, J. Burgueño, J. Fritsche-Neto, R. |
author_facet |
De Oliveira Couto, E.G. Cury, M.N. Bandeira e Sousa, M. Granato, I. Vidotti, M.S. Garbuglio, D.D. Crossa, J. Burgueño, J. Fritsche-Neto, R. |
author_sort |
De Oliveira Couto, E.G. |
title |
Effect of F1 and F2 generations on genetic variability and working steps of doubled haploid production in maize |
title_short |
Effect of F1 and F2 generations on genetic variability and working steps of doubled haploid production in maize |
title_full |
Effect of F1 and F2 generations on genetic variability and working steps of doubled haploid production in maize |
title_fullStr |
Effect of F1 and F2 generations on genetic variability and working steps of doubled haploid production in maize |
title_full_unstemmed |
Effect of F1 and F2 generations on genetic variability and working steps of doubled haploid production in maize |
title_sort |
effect of f1 and f2 generations on genetic variability and working steps of doubled haploid production in maize |
publisher |
Public Library of Science |
publishDate |
2019 |
url |
https://hdl.handle.net/10883/22510 |
work_keys_str_mv |
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