QTL mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties Zhongmai 578 and Jimai 22
Pre-harvest sprouting (PHS) is one of the serious global issues in wheat production. Identification of quantitative trait loci (QTL) and closely-linked markers is greatly helpful for wheat improvement. In the present study, a recombinant inbred line (RIL) population derived from the cross of Zhongmai 578 (ZM578)/Jimai 22 (JM22) and parents were phenotyped in five environments and genotyped by the wheat 50 K single-nucleotide polymorphism (SNP) array. Two QTL of germination index (GI), QGI.caas-3A and QGI.caas-5A, were detected, explaining 4.33%–5.58% and 4.43%–8.02% of the phenotypic variances, respectively. The resistant effect of QGI.caas-3A was contributed by JM22, whereas that of QGI.caas.5A was from ZM578. The two QTL did not correspond to any previously identified genes or genetic loci for PHS-related traits according to their locations in the Chinese Spring reference genome, indicating that they are likely to be new loci for PHS resistance. Four kompetitive allele-specific PCR (KASP) markers K_AX-109605367and K_AX-179559687 flanking QGI.caas-3A, and K_AX-111258240 and K_AX-109402944 flanking QGI.caas-5A, were developed and validated in a natural population of 100 wheat cultivars. The distribution frequency of resistance alleles at Qphs.caas-3A and Qphs.caas-5A loci were 82.7% and 57.1%, respectively, in the natural population. These findings provide new QTL and tightly linked KASP markers for improvement of PHS resistance in wheat.
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2023
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Subjects: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, KASP Markers, PREHARVEST SPROUTING, GERMINATION INDEX, QUANTITATIVE TRAIT LOCI, WHEAT, |
Online Access: | https://hdl.handle.net/10883/22377 |
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dig-cimmyt-10883-223772024-02-13T16:36:46Z QTL mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties Zhongmai 578 and Jimai 22 Shawai, R.S. Dan Liu Lingli Li Chen, T. Ming Li Shuanghe Cao Xianchun Xia Jindong Liu He Zhonghu Yong Zhang AGRICULTURAL SCIENCES AND BIOTECHNOLOGY KASP Markers PREHARVEST SPROUTING GERMINATION INDEX QUANTITATIVE TRAIT LOCI WHEAT Pre-harvest sprouting (PHS) is one of the serious global issues in wheat production. Identification of quantitative trait loci (QTL) and closely-linked markers is greatly helpful for wheat improvement. In the present study, a recombinant inbred line (RIL) population derived from the cross of Zhongmai 578 (ZM578)/Jimai 22 (JM22) and parents were phenotyped in five environments and genotyped by the wheat 50 K single-nucleotide polymorphism (SNP) array. Two QTL of germination index (GI), QGI.caas-3A and QGI.caas-5A, were detected, explaining 4.33%–5.58% and 4.43%–8.02% of the phenotypic variances, respectively. The resistant effect of QGI.caas-3A was contributed by JM22, whereas that of QGI.caas.5A was from ZM578. The two QTL did not correspond to any previously identified genes or genetic loci for PHS-related traits according to their locations in the Chinese Spring reference genome, indicating that they are likely to be new loci for PHS resistance. Four kompetitive allele-specific PCR (KASP) markers K_AX-109605367and K_AX-179559687 flanking QGI.caas-3A, and K_AX-111258240 and K_AX-109402944 flanking QGI.caas-5A, were developed and validated in a natural population of 100 wheat cultivars. The distribution frequency of resistance alleles at Qphs.caas-3A and Qphs.caas-5A loci were 82.7% and 57.1%, respectively, in the natural population. These findings provide new QTL and tightly linked KASP markers for improvement of PHS resistance in wheat. 863–869 2023-01-11T01:20:11Z 2023-01-11T01:20:11Z 2023 Article Published Version https://hdl.handle.net/10883/22377 10.1016/j.cj.2022.12.001 English https://www.sciencedirect.com/science/article/pii/S2214514122002628?via%3Dihub#s0100 CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose Open Access China ICS 3 11 2095-5421 The Crop Journal |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY KASP Markers PREHARVEST SPROUTING GERMINATION INDEX QUANTITATIVE TRAIT LOCI WHEAT AGRICULTURAL SCIENCES AND BIOTECHNOLOGY KASP Markers PREHARVEST SPROUTING GERMINATION INDEX QUANTITATIVE TRAIT LOCI WHEAT |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY KASP Markers PREHARVEST SPROUTING GERMINATION INDEX QUANTITATIVE TRAIT LOCI WHEAT AGRICULTURAL SCIENCES AND BIOTECHNOLOGY KASP Markers PREHARVEST SPROUTING GERMINATION INDEX QUANTITATIVE TRAIT LOCI WHEAT Shawai, R.S. Dan Liu Lingli Li Chen, T. Ming Li Shuanghe Cao Xianchun Xia Jindong Liu He Zhonghu Yong Zhang QTL mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties Zhongmai 578 and Jimai 22 |
description |
Pre-harvest sprouting (PHS) is one of the serious global issues in wheat production. Identification of quantitative trait loci (QTL) and closely-linked markers is greatly helpful for wheat improvement. In the present study, a recombinant inbred line (RIL) population derived from the cross of Zhongmai 578 (ZM578)/Jimai 22 (JM22) and parents were phenotyped in five environments and genotyped by the wheat 50 K single-nucleotide polymorphism (SNP) array. Two QTL of germination index (GI), QGI.caas-3A and QGI.caas-5A, were detected, explaining 4.33%–5.58% and 4.43%–8.02% of the phenotypic variances, respectively. The resistant effect of QGI.caas-3A was contributed by JM22, whereas that of QGI.caas.5A was from ZM578. The two QTL did not correspond to any previously identified genes or genetic loci for PHS-related traits according to their locations in the Chinese Spring reference genome, indicating that they are likely to be new loci for PHS resistance. Four kompetitive allele-specific PCR (KASP) markers K_AX-109605367and K_AX-179559687 flanking QGI.caas-3A, and K_AX-111258240 and K_AX-109402944 flanking QGI.caas-5A, were developed and validated in a natural population of 100 wheat cultivars. The distribution frequency of resistance alleles at Qphs.caas-3A and Qphs.caas-5A loci were 82.7% and 57.1%, respectively, in the natural population. These findings provide new QTL and tightly linked KASP markers for improvement of PHS resistance in wheat. |
format |
Article |
topic_facet |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY KASP Markers PREHARVEST SPROUTING GERMINATION INDEX QUANTITATIVE TRAIT LOCI WHEAT |
author |
Shawai, R.S. Dan Liu Lingli Li Chen, T. Ming Li Shuanghe Cao Xianchun Xia Jindong Liu He Zhonghu Yong Zhang |
author_facet |
Shawai, R.S. Dan Liu Lingli Li Chen, T. Ming Li Shuanghe Cao Xianchun Xia Jindong Liu He Zhonghu Yong Zhang |
author_sort |
Shawai, R.S. |
title |
QTL mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties Zhongmai 578 and Jimai 22 |
title_short |
QTL mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties Zhongmai 578 and Jimai 22 |
title_full |
QTL mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties Zhongmai 578 and Jimai 22 |
title_fullStr |
QTL mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties Zhongmai 578 and Jimai 22 |
title_full_unstemmed |
QTL mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties Zhongmai 578 and Jimai 22 |
title_sort |
qtl mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties zhongmai 578 and jimai 22 |
publisher |
ICS |
publishDate |
2023 |
url |
https://hdl.handle.net/10883/22377 |
work_keys_str_mv |
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