Effect of sperm dosage transportation in stallions: Effect on sperm DNA fragmentation

Artificial insemination programs for horses usually involve ex vivo handling and transporting of sperm. The present experiment was designed to: (i) assess the effect of transportation on sperm DNA integrity at different time post semen collection, and (ii) evaluate if sperm DNA quality deteriorates rapidly beyond 24 h of cooled storage. After collection, the ejaculates were extended using INRA 96 and semen was prepared for prompt analysis (A0) or 24 h/48 h cooled-shipping (B24 and C48 respectively). Each sample was assessed for sperm DNA fragmentation index (SDFI) at time 0 and after incubation for 2, 6 and 24 h at 37 °C. There was very little difference in SDFI between freshly extended (A0) and 24 h/48 h cooled-transported semen samples (B24/C48) at time 0. After 2 h of incubation at 37 °C, there was an increase in SDFI ranging from 2.7% to 7.5% per hour in freshly extended semen samples (A0: 5.1 ± 1.5), while cooled-transported semen samples had a much greater increase in SDFI, ranging from 5.0% to 20.5% (B24: 14.7 ± 5.6) and from 8.2% to 26.8% (C48: 18.3 ± 7.2) respectively. There were not marked differences in the sperm DNA integrity between 24 and 48 h for transported samples, thus there is the possibility of desirable fertility with use of stallion sperm after 48 h of cooled storage.

Bibliographic Details

Main Authors:	Torre, J. de la, Crespo, F., Arroyo, F., Zabal-Aguirre, M., Abdoon, A. S., Gosálvez, J.
Other Authors:	Ministerio de Asuntos Exteriores (España)
Format:	artículo biblioteca
Published:	Elsevier 2019-07
Subjects:	Stallion reproduction, Sperm quality, Sperm DNA fragmentation, Refrigeration, Cooled semen,
Online Access:	http://hdl.handle.net/10261/201348 http://dx.doi.org/10.13039/501100003329
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