Ultrasensitive detection of soy traces by immunosensing of glycinin and β-conglycinin at disposable electrochemical platforms
This work reports the first electrochemical bioplatform for the determination of soy traces in food. The bioplatform involves sandwich-type immunoassays using specific antibodies for β-conglycinin and glycinin, which are the main allergenic soy proteins, and carboxylic acid-modified magnetic microbeads. Amperometric detection at −0.20 V (vs. an Ag pseudo-reference electrode) was performed using single or dual screen-printed carbon electrodes and the H2O2/hydroquinone (HQ) system. The measured variation in the cathodic current was directly proportional to the concentration of target allergenic proteins. The developed bioplatforms exhibit a good selectivity and sensitivity providing limits of detection (LOD) values of 0.03 and 0.02 ng mL−1 for β-conglycinin and glycinin, respectively. The determination of both proteins can be carried out in only 1.5 h. The electrochemical bioplatforms allow their accurate determinations (with results statistically comparable to those provided by ELISA methodologies) in raw cookie dough and baked cookies enriched with soy flour. The results obtained confirm, in a pioneering way with electrochemical biosensors, the possibility of discriminating samples incurred with as little as 0.0005 ppm of a food allergen in model cookie extracts.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2022
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Subjects: | Soy, β-conglycinin, Glycinin, Immunoplatform, Electrochemical, Cookies, |
Online Access: | http://hdl.handle.net/10261/278955 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100004837 |
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dig-cial-es-10261-2789552022-11-04T16:43:04Z Ultrasensitive detection of soy traces by immunosensing of glycinin and β-conglycinin at disposable electrochemical platforms Blázquez-García, Marina Arévalo, Beatriz Serafín, Verónica Benedé, Sara Mata, Luis Galán-Malo, Patricia Segura-Gil, Isabel Pérez, María Dolores Pingarrón, José M. Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Ministerio de Economía, Industria y Competitividad (España) Ministerio de Ciencia e Innovación (España) Comunidad de Madrid Soy β-conglycinin Glycinin Immunoplatform Electrochemical Cookies This work reports the first electrochemical bioplatform for the determination of soy traces in food. The bioplatform involves sandwich-type immunoassays using specific antibodies for β-conglycinin and glycinin, which are the main allergenic soy proteins, and carboxylic acid-modified magnetic microbeads. Amperometric detection at −0.20 V (vs. an Ag pseudo-reference electrode) was performed using single or dual screen-printed carbon electrodes and the H2O2/hydroquinone (HQ) system. The measured variation in the cathodic current was directly proportional to the concentration of target allergenic proteins. The developed bioplatforms exhibit a good selectivity and sensitivity providing limits of detection (LOD) values of 0.03 and 0.02 ng mL−1 for β-conglycinin and glycinin, respectively. The determination of both proteins can be carried out in only 1.5 h. The electrochemical bioplatforms allow their accurate determinations (with results statistically comparable to those provided by ELISA methodologies) in raw cookie dough and baked cookies enriched with soy flour. The results obtained confirm, in a pioneering way with electrochemical biosensors, the possibility of discriminating samples incurred with as little as 0.0005 ppm of a food allergen in model cookie extracts. The financial support of PID2019-103899RB-I00 to S.C., (Spanish Ministerio de Ciencia e Innovación) Research Project, and TRANSNANOAVANSENS-CM Program from the Comunidad de Madrid (Grant S2018/NMT-4349) to S.C. are gratefully acknowledged. S.B. acknowledges a Juan de la Cierva Incorporación contract (Ref.: IJCI-2017-31345) from the Spanish Ministerio de Economía, Industria y Competitividad. Peer reviewed 2022-09-12T12:51:47Z 2022-09-12T12:51:47Z 2022 artículo Talanta 241: 123226 (2022) 0039-9140 http://hdl.handle.net/10261/278955 10.1016/j.talanta.2022.123226 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100004837 35066283 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-103899RB-I00/ES/BIOSENSORES PARA DESENTRAÑAR EL LEGADO Y EL FUTURO DE LA EPIGENETICA Y LA METASTASIS DEL CANCER/ S2018/NMT-4349/TRANSNANOAVANSENS-CM info:eu-repo/grantAgreement/AEI//IJCI-2017-31345 Publisher's version https://doi.org/10.1016/j.talanta.2022.123226 Sí open Elsevier |
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Soy β-conglycinin Glycinin Immunoplatform Electrochemical Cookies Soy β-conglycinin Glycinin Immunoplatform Electrochemical Cookies |
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Soy β-conglycinin Glycinin Immunoplatform Electrochemical Cookies Soy β-conglycinin Glycinin Immunoplatform Electrochemical Cookies Blázquez-García, Marina Arévalo, Beatriz Serafín, Verónica Benedé, Sara Mata, Luis Galán-Malo, Patricia Segura-Gil, Isabel Pérez, María Dolores Pingarrón, José M. Ultrasensitive detection of soy traces by immunosensing of glycinin and β-conglycinin at disposable electrochemical platforms |
description |
This work reports the first electrochemical bioplatform for the determination of soy traces in food. The bioplatform involves sandwich-type immunoassays using specific antibodies for β-conglycinin and glycinin, which are the main allergenic soy proteins, and carboxylic acid-modified magnetic microbeads. Amperometric detection at −0.20 V (vs. an Ag pseudo-reference electrode) was performed using single or dual screen-printed carbon electrodes and the H2O2/hydroquinone (HQ) system. The measured variation in the cathodic current was directly proportional to the concentration of target allergenic proteins. The developed bioplatforms exhibit a good selectivity and sensitivity providing limits of detection (LOD) values of 0.03 and 0.02 ng mL−1 for β-conglycinin and glycinin, respectively. The determination of both proteins can be carried out in only 1.5 h. The electrochemical bioplatforms allow their accurate determinations (with results statistically comparable to those provided by ELISA methodologies) in raw cookie dough and baked cookies enriched with soy flour. The results obtained confirm, in a pioneering way with electrochemical biosensors, the possibility of discriminating samples incurred with as little as 0.0005 ppm of a food allergen in model cookie extracts. |
author2 |
Ministerio de Ciencia, Innovación y Universidades (España) |
author_facet |
Ministerio de Ciencia, Innovación y Universidades (España) Blázquez-García, Marina Arévalo, Beatriz Serafín, Verónica Benedé, Sara Mata, Luis Galán-Malo, Patricia Segura-Gil, Isabel Pérez, María Dolores Pingarrón, José M. |
format |
artículo |
topic_facet |
Soy β-conglycinin Glycinin Immunoplatform Electrochemical Cookies |
author |
Blázquez-García, Marina Arévalo, Beatriz Serafín, Verónica Benedé, Sara Mata, Luis Galán-Malo, Patricia Segura-Gil, Isabel Pérez, María Dolores Pingarrón, José M. |
author_sort |
Blázquez-García, Marina |
title |
Ultrasensitive detection of soy traces by immunosensing of glycinin and β-conglycinin at disposable electrochemical platforms |
title_short |
Ultrasensitive detection of soy traces by immunosensing of glycinin and β-conglycinin at disposable electrochemical platforms |
title_full |
Ultrasensitive detection of soy traces by immunosensing of glycinin and β-conglycinin at disposable electrochemical platforms |
title_fullStr |
Ultrasensitive detection of soy traces by immunosensing of glycinin and β-conglycinin at disposable electrochemical platforms |
title_full_unstemmed |
Ultrasensitive detection of soy traces by immunosensing of glycinin and β-conglycinin at disposable electrochemical platforms |
title_sort |
ultrasensitive detection of soy traces by immunosensing of glycinin and β-conglycinin at disposable electrochemical platforms |
publisher |
Elsevier |
publishDate |
2022 |
url |
http://hdl.handle.net/10261/278955 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100004837 |
work_keys_str_mv |
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1777671551732154368 |