Effect of lyso-Gb3 on the intestinal microbiota may contribute to gastrointestinal symptoms in Fabry’s disease patients

Effect of lyso-Gb3 on the intestinal microbiota may contribute to gastrointestinal symptoms in Fabry’s disease patients

Resumen del trabajo presentado al 29th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), celebrado en Amsterdam (Netherlands) del 13 al 16 de abril de 2019.

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Main Authors: Aguilera-Correa, John-Jairo, Sánchez-Niño, María Dolores, Ortiz, Alberto, Sáez Martínez, E. F., Martínez-Cuesta, M. Carmen, Peláez, Carmen, Esteban, Jaime, Requena, Teresa
Format: póster de congreso biblioteca
Language:English
Published: 2019
Online Access:http://hdl.handle.net/10261/205778
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spelling dig-cial-es-10261-2057782020-03-31T01:03:08Z Effect of lyso-Gb3 on the intestinal microbiota may contribute to gastrointestinal symptoms in Fabry’s disease patients Aguilera-Correa, John-Jairo Sánchez-Niño, María Dolores Ortiz, Alberto Sáez Martínez, E. F. Martínez-Cuesta, M. Carmen Peláez, Carmen Esteban, Jaime Requena, Teresa Resumen del trabajo presentado al 29th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), celebrado en Amsterdam (Netherlands) del 13 al 16 de abril de 2019. [Background]: Fabry’s disease is an X-linked, lysosomal storage disorder due to α-galactosidase A deficiency, characterized by the lysosomal accumulation of glycosphingolipids, mainly globotriaosylceramide (Gb3) and its deacetylated form as globotriaosylsphingosine (lyso-Gb3). Some of the disease symptoms affect the gastrointestinal tract, causing nausea, vomiting, diarrhea, constipation and difficulty in gaining weight. Certain colonic alterations such as polyps and cancer are frequent in these patients. Lyso-Gb3 accumulates in different organs, including liver and intestines, and plasma. We hypothesized that bile secretion may result in Lyso-Gb3 intestinal concentration exceeding that in plasma (500 nM), which could alter the intestinal microbiota composition and the development of gastrointestinal symptoms. In this study, we describe the effect of lyso-Gb3 on the growth of human intestinal microbiota. [Materials/methods]: Microbiota from pooled faeces of healthy individuals was stabilized in a dynamic simulator of the gastrointestinal tract (LWT-Food Sci Technol, 2015, 61:283) and incubated in presence or not (control) of 500 nM of lyso-Gb3. After incubation under strict anaerobic conditions for 24 h at 37 ºC, the bacterial concentration (log copy number/mL) was estimated through quantitative real time polymerase chain reaction (qPCR) using specific primers for bacterial groups representative of the human gut microbiota. Data were analyzed by t-Student test. Values are tabulated as mean and standard deviation. [Results]: Lyso-Gb3 significantly modified the growth of different bacterial groups of the human intestinal microbiota. [Conclusions]: Lyso-Gb3 affects the intestinal microbiota composition. In particular, it favours the growth of Bacteroides fragilis that may be responsible of colonic alterations such as colon polyps and cancer. Peer reviewed 2020-03-30T10:30:26Z 2020-03-30T10:30:26Z 2019 póster de congreso http://purl.org/coar/resource_type/c_6670 29th European Congress of Clinical Microbiology and Infectious Diseases (2019) http://hdl.handle.net/10261/205778 en Sí none
institution CIAL ES
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country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-cial-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del CIAL España
language English
description Resumen del trabajo presentado al 29th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), celebrado en Amsterdam (Netherlands) del 13 al 16 de abril de 2019.
format póster de congreso
author Aguilera-Correa, John-Jairo
Sánchez-Niño, María Dolores
Ortiz, Alberto
Sáez Martínez, E. F.
Martínez-Cuesta, M. Carmen
Peláez, Carmen
Esteban, Jaime
Requena, Teresa
spellingShingle Aguilera-Correa, John-Jairo
Sánchez-Niño, María Dolores
Ortiz, Alberto
Sáez Martínez, E. F.
Martínez-Cuesta, M. Carmen
Peláez, Carmen
Esteban, Jaime
Requena, Teresa
Effect of lyso-Gb3 on the intestinal microbiota may contribute to gastrointestinal symptoms in Fabry’s disease patients
author_facet Aguilera-Correa, John-Jairo
Sánchez-Niño, María Dolores
Ortiz, Alberto
Sáez Martínez, E. F.
Martínez-Cuesta, M. Carmen
Peláez, Carmen
Esteban, Jaime
Requena, Teresa
author_sort Aguilera-Correa, John-Jairo
title Effect of lyso-Gb3 on the intestinal microbiota may contribute to gastrointestinal symptoms in Fabry’s disease patients
title_short Effect of lyso-Gb3 on the intestinal microbiota may contribute to gastrointestinal symptoms in Fabry’s disease patients
title_full Effect of lyso-Gb3 on the intestinal microbiota may contribute to gastrointestinal symptoms in Fabry’s disease patients
title_fullStr Effect of lyso-Gb3 on the intestinal microbiota may contribute to gastrointestinal symptoms in Fabry’s disease patients
title_full_unstemmed Effect of lyso-Gb3 on the intestinal microbiota may contribute to gastrointestinal symptoms in Fabry’s disease patients
title_sort effect of lyso-gb3 on the intestinal microbiota may contribute to gastrointestinal symptoms in fabry’s disease patients
publishDate 2019
url http://hdl.handle.net/10261/205778
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