Allium porrum extract decreases effector cell degranulation and modulates airway epithelial cell function
This article belongs to the Special Issue Edible Fruits and Human Health.
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Multidisciplinary Digital Publishing Institute
2019-06-08
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| Subjects: | Allium porrum, Antioxidant activity, Phenolic compounds, Mast cell, Epithelium, |
| Online Access: | http://hdl.handle.net/10261/185049 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100011033 |
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dig-cial-es-10261-1850492021-12-28T16:04:34Z Allium porrum extract decreases effector cell degranulation and modulates airway epithelial cell function Benedé, Sara Gradillas, Ana Villalba, Mayte Batanero, Eva Ministerio de Economía y Competitividad (España) Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Allium porrum Antioxidant activity Phenolic compounds Mast cell Epithelium This article belongs to the Special Issue Edible Fruits and Human Health. Allium genus plants, such as leek (Allium porrum), are rich sources of anti-inflammatory and anti-oxidant secondary metabolites; this is of interest because it demonstrates their suitability as pharmacological alternatives for inflammatory processes, including allergy treatment. The composition of methanolic leek extract (LE) was analyzed by GC–MS and LC–IT/MS, and the total phenolic content and antioxidant capacity were quantified by colorimetric methods. Its pharmacological potential was analyzed in human bronchial epithelial Calu-3 cells, human mast cells LAD2, and humanized rat basophiles RBL-2H3. LE exhibited a cytotoxic effect on Calu-3 cells and HumRBL-2H3 cells only at high concentrations and in a dose-dependent manner. Moreover, LE decreased the degranulation of LAD2 and HumRBL-2H3 cells. LE treatment also significantly prevented alterations in transepithelial electrical resistance values and mRNA levels of glutathione-S-transferase (GST), c-Jun, and NFκB after treatment with H2O2 in ALI-cultured Calu-3 cells. Finally, ALI-cultured Calu-3 cells treated with LE showed lower permeability to Ole e 1 compared to untreated cells. A reduction in IL-6 secretion in ALI-cultured Calu-3 cells treated with LE was also observed. In summary, the results obtained in this work suggest that A. porrum extract may have potential anti-allergic effects due to its antioxidant and anti-inflammatory properties. This study provides several important insights into how LE can protect against allergy. This work was supported by grants from the Spanish Ministry of Economy and Competitivity SAF2014-53209-R, SAF2017-86483-R, PI15/00107 and RIRAAF Network RD16/0006/0014 from the ISCIII. S.B. is supported by a Juan de la Cierva Formación contract from the Spanish Ministerio de Economía. Peer reviewed 2019-06-28T06:41:01Z 2019-06-28T06:41:01Z 2019-06-08 2019-06-28T06:41:01Z artículo http://purl.org/coar/resource_type/c_6501 Nutrients 11(6): 1303 (2019) 2072-6643 http://hdl.handle.net/10261/185049 10.3390/nu11061303 2072-6643 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100011033 31181780 #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2014-53209-R info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/SAF2017-86483-R SAF2017-86483-R/AEI/10.13039/501100011033 Publisher’s version https://doi.org/10.3390/nu11061303 Sí open Multidisciplinary Digital Publishing Institute |
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Allium porrum Antioxidant activity Phenolic compounds Mast cell Epithelium Allium porrum Antioxidant activity Phenolic compounds Mast cell Epithelium |
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Allium porrum Antioxidant activity Phenolic compounds Mast cell Epithelium Allium porrum Antioxidant activity Phenolic compounds Mast cell Epithelium Benedé, Sara Gradillas, Ana Villalba, Mayte Batanero, Eva Allium porrum extract decreases effector cell degranulation and modulates airway epithelial cell function |
| description |
This article belongs to the Special Issue Edible Fruits and Human Health. |
| author2 |
Ministerio de Economía y Competitividad (España) |
| author_facet |
Ministerio de Economía y Competitividad (España) Benedé, Sara Gradillas, Ana Villalba, Mayte Batanero, Eva |
| format |
artículo |
| topic_facet |
Allium porrum Antioxidant activity Phenolic compounds Mast cell Epithelium |
| author |
Benedé, Sara Gradillas, Ana Villalba, Mayte Batanero, Eva |
| author_sort |
Benedé, Sara |
| title |
Allium porrum extract decreases effector cell degranulation and modulates airway epithelial cell function |
| title_short |
Allium porrum extract decreases effector cell degranulation and modulates airway epithelial cell function |
| title_full |
Allium porrum extract decreases effector cell degranulation and modulates airway epithelial cell function |
| title_fullStr |
Allium porrum extract decreases effector cell degranulation and modulates airway epithelial cell function |
| title_full_unstemmed |
Allium porrum extract decreases effector cell degranulation and modulates airway epithelial cell function |
| title_sort |
allium porrum extract decreases effector cell degranulation and modulates airway epithelial cell function |
| publisher |
Multidisciplinary Digital Publishing Institute |
| publishDate |
2019-06-08 |
| url |
http://hdl.handle.net/10261/185049 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100011033 |
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