Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation

Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation

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The aim was to investigate the potential of germinated soybean proteins as a source of peptides with anticancer and anti-inflammatory activities produced after simulated gastrointestinal digestion. Protein concentrate from germinated soybean was hydrolysed with pepsin/pancreatin and fractionated by ultrafiltration. Whole digest and fractions >10, 5-10 and <5 kDa caused cytotoxicity to Caco-2, HT-29, HCT-116 human colon cancer cells, and reduced inflammatory response caused by lipopolysaccharide in macrophages RAW267.4. Antiproliferative and anti-inflammatory effects were generally higher in 5-10 kDa fractions. This fraction was further purified by semi-preparative chromatography and characterised by HPLC-MS/MS. The most potent fraction was mainly composed of ß-conglycinin and glycinin fragments rich in glutamine. This is the first report on the anti-cancer and anti-inflammatory effects of newly isolated and identified peptides from germinated soybean released during gastrointestinal digestion. These findings highlight the potential of germination as a process to obtain functional foods or nutraceuticals for colon cancer prevention.

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Bibliographic Details
Main Authors: González-Montoya, Marcela, Hernández-Ledesma, Blanca, Silván, José Manuel, Mora-Escobedo, Rosalva, Martínez Villaluenga, Cristina
Other Authors: Consejo Nacional de Ciencia y Tecnología (México)
Format: artículo biblioteca
Published: Elsevier 2018
Subjects:Peptides, Antiproliferative, Anti-inflammatory, Digestion, Soybean, Germination,
Online Access:http://hdl.handle.net/10261/171032
http://dx.doi.org/10.13039/501100003069
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003141
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spelling dig-cial-es-10261-1710322020-12-13T09:22:06Z Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation González-Montoya, Marcela Hernández-Ledesma, Blanca Silván, José Manuel Mora-Escobedo, Rosalva Martínez Villaluenga, Cristina Consejo Nacional de Ciencia y Tecnología (México) Instituto Politécnico Nacional (México) European Commission Peptides Antiproliferative Anti-inflammatory Digestion Soybean Germination The aim was to investigate the potential of germinated soybean proteins as a source of peptides with anticancer and anti-inflammatory activities produced after simulated gastrointestinal digestion. Protein concentrate from germinated soybean was hydrolysed with pepsin/pancreatin and fractionated by ultrafiltration. Whole digest and fractions >10, 5-10 and <5 kDa caused cytotoxicity to Caco-2, HT-29, HCT-116 human colon cancer cells, and reduced inflammatory response caused by lipopolysaccharide in macrophages RAW267.4. Antiproliferative and anti-inflammatory effects were generally higher in 5-10 kDa fractions. This fraction was further purified by semi-preparative chromatography and characterised by HPLC-MS/MS. The most potent fraction was mainly composed of ß-conglycinin and glycinin fragments rich in glutamine. This is the first report on the anti-cancer and anti-inflammatory effects of newly isolated and identified peptides from germinated soybean released during gastrointestinal digestion. These findings highlight the potential of germination as a process to obtain functional foods or nutraceuticals for colon cancer prevention. This work was supported by the Ministry of Economy and Competitiveness (MINECO, Spain) (grant numbers AGL2013-43247-R and AGL2015-66886-R) and EU (FEDER program). M. G. M. and R. M. E. acknowledge the financial support of SEP-CONACyT project No. 242860 and the Instituto Politecnico Nacional. M. G. M. for the scholarship (CONACyT No. 291062). Peer Reviewed 2018-10-15T10:24:07Z 2018-10-15T10:24:07Z 2018 2018-10-15T10:24:09Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.foodchem.2017.09.035 issn: 0308-8146 Food Chemistry 242: 75-82 (2018) http://hdl.handle.net/10261/171032 10.1016/j.foodchem.2017.09.035 http://dx.doi.org/10.13039/501100003069 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003141 29037738 #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-43247-R info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-66886-R Postprint https://doi.org/10.1016/j.foodchem.2017.09.035 Sí open Elsevier
institution CIAL ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-cial-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del CIAL España
topic Peptides
Antiproliferative
Anti-inflammatory
Digestion
Soybean
Germination
Peptides
Antiproliferative
Anti-inflammatory
Digestion
Soybean
Germination
spellingShingle Peptides
Antiproliferative
Anti-inflammatory
Digestion
Soybean
Germination
Peptides
Antiproliferative
Anti-inflammatory
Digestion
Soybean
Germination
González-Montoya, Marcela
Hernández-Ledesma, Blanca
Silván, José Manuel
Mora-Escobedo, Rosalva
Martínez Villaluenga, Cristina
Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation
description The aim was to investigate the potential of germinated soybean proteins as a source of peptides with anticancer and anti-inflammatory activities produced after simulated gastrointestinal digestion. Protein concentrate from germinated soybean was hydrolysed with pepsin/pancreatin and fractionated by ultrafiltration. Whole digest and fractions >10, 5-10 and <5 kDa caused cytotoxicity to Caco-2, HT-29, HCT-116 human colon cancer cells, and reduced inflammatory response caused by lipopolysaccharide in macrophages RAW267.4. Antiproliferative and anti-inflammatory effects were generally higher in 5-10 kDa fractions. This fraction was further purified by semi-preparative chromatography and characterised by HPLC-MS/MS. The most potent fraction was mainly composed of ß-conglycinin and glycinin fragments rich in glutamine. This is the first report on the anti-cancer and anti-inflammatory effects of newly isolated and identified peptides from germinated soybean released during gastrointestinal digestion. These findings highlight the potential of germination as a process to obtain functional foods or nutraceuticals for colon cancer prevention.
author2 Consejo Nacional de Ciencia y Tecnología (México)
author_facet Consejo Nacional de Ciencia y Tecnología (México)
González-Montoya, Marcela
Hernández-Ledesma, Blanca
Silván, José Manuel
Mora-Escobedo, Rosalva
Martínez Villaluenga, Cristina
format artículo
topic_facet Peptides
Antiproliferative
Anti-inflammatory
Digestion
Soybean
Germination
author González-Montoya, Marcela
Hernández-Ledesma, Blanca
Silván, José Manuel
Mora-Escobedo, Rosalva
Martínez Villaluenga, Cristina
author_sort González-Montoya, Marcela
title Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation
title_short Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation
title_full Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation
title_fullStr Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation
title_full_unstemmed Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation
title_sort peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation
publisher Elsevier
publishDate 2018
url http://hdl.handle.net/10261/171032
http://dx.doi.org/10.13039/501100003069
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003141
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AT silvanjosemanuel peptidesderivedfrominvitrogastrointestinaldigestionofgerminatedsoybeanproteinsinhibithumancoloncancercellsproliferationandinflammation
AT moraescobedorosalva peptidesderivedfrominvitrogastrointestinaldigestionofgerminatedsoybeanproteinsinhibithumancoloncancercellsproliferationandinflammation
AT martinezvillaluengacristina peptidesderivedfrominvitrogastrointestinaldigestionofgerminatedsoybeanproteinsinhibithumancoloncancercellsproliferationandinflammation
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