A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers
Sweetpotato (Ipomoea batatas (L.) Lam.), a hexaploid outcrossing crop, is an important staple and food security crop in developing countries in Africa and Asia. The availability of genomic resources for sweetpotato is in striking contrast to its importance for human nutrition. Previously existing sequence data were restricted to around 22,000 expressed sequence tag (EST) sequences and ~ 1,500 GenBank sequences. We have used 454 pyrosequencing to augment the available gene sequence information to enhance functional genomics and marker design for this plant species.
Main Authors: | , , , , , , , , , , , , , , |
---|---|
Format: | Journal Article biblioteca |
Language: | English |
Published: |
Springer
2010-12
|
Subjects: | sweet potatoes, genes, nucleotide sequence, genetic markers, |
Online Access: | https://hdl.handle.net/10568/97198 https://doi.org/10.1186/1471-2164-11-604 http://dx.doi.org/10.21223/P3/CZV6P9 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cgspace-10568-97198 |
---|---|
record_format |
koha |
spelling |
dig-cgspace-10568-971982023-12-08T19:36:04Z A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers Schafleitner, R. Tincopa, L.R. Palomino, O. Rossel, G. Robles, R. Alagon, R. Rivera, C. Quispe, C. Rojas, L. Pacheco, J. Solis, J. Cerna, D. Kim, J.Y. Hou, J. Simon, R. sweet potatoes genes nucleotide sequence genetic markers Sweetpotato (Ipomoea batatas (L.) Lam.), a hexaploid outcrossing crop, is an important staple and food security crop in developing countries in Africa and Asia. The availability of genomic resources for sweetpotato is in striking contrast to its importance for human nutrition. Previously existing sequence data were restricted to around 22,000 expressed sequence tag (EST) sequences and ~ 1,500 GenBank sequences. We have used 454 pyrosequencing to augment the available gene sequence information to enhance functional genomics and marker design for this plant species. 2010-12 2018-09-13T13:55:33Z 2018-09-13T13:55:33Z Journal Article Schafleitner, R.; Tincopa, L.R.; Palomino, O.; Rossel, G.; Robles, R.; Alagon, R.; Rivera, C.; Quispe, C.; Rojas, L.; Pacheco, J.; Solis, J.; Cerna, D.; Kim, J.Y.; Hou, J.; Simon, R. 2010. A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers. BMC Genomics. ISSN 1471-2164. 11:604. 10 p. 1471-2164 https://hdl.handle.net/10568/97198 https://doi.org/10.1186/1471-2164-11-604 http://dx.doi.org/10.21223/P3/CZV6P9 en CC-BY-2.0 Open Access 10 p. Springer BMC Genomics |
institution |
CGIAR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cgspace |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CGIAR |
language |
English |
topic |
sweet potatoes genes nucleotide sequence genetic markers sweet potatoes genes nucleotide sequence genetic markers |
spellingShingle |
sweet potatoes genes nucleotide sequence genetic markers sweet potatoes genes nucleotide sequence genetic markers Schafleitner, R. Tincopa, L.R. Palomino, O. Rossel, G. Robles, R. Alagon, R. Rivera, C. Quispe, C. Rojas, L. Pacheco, J. Solis, J. Cerna, D. Kim, J.Y. Hou, J. Simon, R. A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers |
description |
Sweetpotato (Ipomoea batatas (L.) Lam.), a hexaploid outcrossing crop, is an important staple and food security crop in developing countries in Africa and Asia. The availability of genomic resources for sweetpotato is in striking contrast to its importance for human nutrition. Previously existing sequence data were restricted to around 22,000 expressed sequence tag (EST) sequences and ~ 1,500 GenBank sequences. We have used 454 pyrosequencing to augment the available gene sequence information to enhance functional genomics and marker design for this plant species. |
format |
Journal Article |
topic_facet |
sweet potatoes genes nucleotide sequence genetic markers |
author |
Schafleitner, R. Tincopa, L.R. Palomino, O. Rossel, G. Robles, R. Alagon, R. Rivera, C. Quispe, C. Rojas, L. Pacheco, J. Solis, J. Cerna, D. Kim, J.Y. Hou, J. Simon, R. |
author_facet |
Schafleitner, R. Tincopa, L.R. Palomino, O. Rossel, G. Robles, R. Alagon, R. Rivera, C. Quispe, C. Rojas, L. Pacheco, J. Solis, J. Cerna, D. Kim, J.Y. Hou, J. Simon, R. |
author_sort |
Schafleitner, R. |
title |
A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers |
title_short |
A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers |
title_full |
A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers |
title_fullStr |
A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers |
title_full_unstemmed |
A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers |
title_sort |
sweetpotato gene index established by de novo assembly of pyrosequencing and sanger sequences and mining for gene-based microsatellite markers |
publisher |
Springer |
publishDate |
2010-12 |
url |
https://hdl.handle.net/10568/97198 https://doi.org/10.1186/1471-2164-11-604 http://dx.doi.org/10.21223/P3/CZV6P9 |
work_keys_str_mv |
AT schafleitnerr asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT tincopalr asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT palominoo asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT rosselg asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT roblesr asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT alagonr asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT riverac asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT quispec asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT rojasl asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT pachecoj asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT solisj asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT cernad asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT kimjy asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT houj asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT simonr asweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT schafleitnerr sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT tincopalr sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT palominoo sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT rosselg sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT roblesr sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT alagonr sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT riverac sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT quispec sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT rojasl sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT pachecoj sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT solisj sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT cernad sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT kimjy sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT houj sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers AT simonr sweetpotatogeneindexestablishedbydenovoassemblyofpyrosequencingandsangersequencesandminingforgenebasedmicrosatellitemarkers |
_version_ |
1787229297220517888 |