Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Mull. Arg.) in Uganda
Cassava (Manihot esculenta) growing in Uganda during 2001–2002 has been screened for the presence of begomoviruses using PCR-RFLP, cloning full-length genomic components and nucleotide sequence analysis. In contrast with a recent survey in neighbouring Kenya, which identified three distinct strains of East African cassava mosaic virus (EACMV, EACMV-UG and EACMV-KE2) as well as East African cassava mosaic Zanzibar virus and the new species East African cassava mosaic Kenya virus, only EACMV-UG and, to a lesser extent, African cassava mosaic virus (ACMV) were found associated with cassava in Uganda. The integrity of the cloned genomic components of representative virus isolates was confirmed by demonstrating their infectivity in Nicotiana benthamiana and cassava using biolistic inoculation, providing a convenient means to screen cassava varieties for disease resistance. Both EACMV-UG and ACMV were also associated with Manihot glaziovii. Infectivity studies using cloned components confirmed that viruses from one host could infect the other, suggesting that this wild relative of cassava might be a reservoir host for the disease. The relatively low level of diversity of begomoviruses associated with cassava mosaic disease in Uganda is consistent with reports that EACMV-UG has displaced other begomovirus species and strains during the recent epidemic that swept through the country.
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| Formato: | Journal Article biblioteca |
| Idioma: | English |
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Microbiology Society
2008-07-01
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| Assuntos: | african cassava mosaic virus, begomovirus, dna-a segment, east african cassava mosaic virus, nucleotide, |
| Acesso em linha: | https://hdl.handle.net/10568/90781 https://doi.org/10.1099/vir.0.83637-0 |
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dig-cgspace-10568-907812023-09-09T00:50:32Z Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Mull. Arg.) in Uganda Sserubombwe, W.S. Briddon, R.W. Baguma, Yona K. Ssemakula, G.N. Bull, S.E. Bua, A. Alicai, Titus Omongo, C. Otim-Nape, G.W. Stanley, J. african cassava mosaic virus begomovirus dna-a segment east african cassava mosaic virus nucleotide Cassava (Manihot esculenta) growing in Uganda during 2001–2002 has been screened for the presence of begomoviruses using PCR-RFLP, cloning full-length genomic components and nucleotide sequence analysis. In contrast with a recent survey in neighbouring Kenya, which identified three distinct strains of East African cassava mosaic virus (EACMV, EACMV-UG and EACMV-KE2) as well as East African cassava mosaic Zanzibar virus and the new species East African cassava mosaic Kenya virus, only EACMV-UG and, to a lesser extent, African cassava mosaic virus (ACMV) were found associated with cassava in Uganda. The integrity of the cloned genomic components of representative virus isolates was confirmed by demonstrating their infectivity in Nicotiana benthamiana and cassava using biolistic inoculation, providing a convenient means to screen cassava varieties for disease resistance. Both EACMV-UG and ACMV were also associated with Manihot glaziovii. Infectivity studies using cloned components confirmed that viruses from one host could infect the other, suggesting that this wild relative of cassava might be a reservoir host for the disease. The relatively low level of diversity of begomoviruses associated with cassava mosaic disease in Uganda is consistent with reports that EACMV-UG has displaced other begomovirus species and strains during the recent epidemic that swept through the country. 2008-07-01 2018-02-06T12:14:43Z 2018-02-06T12:14:43Z Journal Article Sserubombwe, W.S., Briddon, R.W., Baguma, Y.K., Ssemakula, G.N., Bull, S.E., Bua, A., ... & Stanley, J. (2008). Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Müll. Arg.) in Uganda. Journal of General Virology, 89(7), 1759-1769. 0022-1317 https://hdl.handle.net/10568/90781 https://doi.org/10.1099/vir.0.83637-0 en Limited Access Microbiology Society |
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african cassava mosaic virus begomovirus dna-a segment east african cassava mosaic virus nucleotide african cassava mosaic virus begomovirus dna-a segment east african cassava mosaic virus nucleotide |
| spellingShingle |
african cassava mosaic virus begomovirus dna-a segment east african cassava mosaic virus nucleotide african cassava mosaic virus begomovirus dna-a segment east african cassava mosaic virus nucleotide Sserubombwe, W.S. Briddon, R.W. Baguma, Yona K. Ssemakula, G.N. Bull, S.E. Bua, A. Alicai, Titus Omongo, C. Otim-Nape, G.W. Stanley, J. Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Mull. Arg.) in Uganda |
| description |
Cassava (Manihot esculenta) growing in Uganda during 2001–2002 has been screened for the
presence of begomoviruses using PCR-RFLP, cloning full-length genomic components and
nucleotide sequence analysis. In contrast with a recent survey in neighbouring Kenya, which
identified three distinct strains of East African cassava mosaic virus (EACMV, EACMV-UG and
EACMV-KE2) as well as East African cassava mosaic Zanzibar virus and the new species East
African cassava mosaic Kenya virus, only EACMV-UG and, to a lesser extent, African cassava
mosaic virus (ACMV) were found associated with cassava in Uganda. The integrity of the cloned
genomic components of representative virus isolates was confirmed by demonstrating their
infectivity in Nicotiana benthamiana and cassava using biolistic inoculation, providing a convenient
means to screen cassava varieties for disease resistance. Both EACMV-UG and ACMV were also
associated with Manihot glaziovii. Infectivity studies using cloned components confirmed that
viruses from one host could infect the other, suggesting that this wild relative of cassava might be
a reservoir host for the disease. The relatively low level of diversity of begomoviruses associated
with cassava mosaic disease in Uganda is consistent with reports that EACMV-UG has
displaced other begomovirus species and strains during the recent epidemic that swept through
the country. |
| format |
Journal Article |
| topic_facet |
african cassava mosaic virus begomovirus dna-a segment east african cassava mosaic virus nucleotide |
| author |
Sserubombwe, W.S. Briddon, R.W. Baguma, Yona K. Ssemakula, G.N. Bull, S.E. Bua, A. Alicai, Titus Omongo, C. Otim-Nape, G.W. Stanley, J. |
| author_facet |
Sserubombwe, W.S. Briddon, R.W. Baguma, Yona K. Ssemakula, G.N. Bull, S.E. Bua, A. Alicai, Titus Omongo, C. Otim-Nape, G.W. Stanley, J. |
| author_sort |
Sserubombwe, W.S. |
| title |
Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Mull. Arg.) in Uganda |
| title_short |
Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Mull. Arg.) in Uganda |
| title_full |
Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Mull. Arg.) in Uganda |
| title_fullStr |
Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Mull. Arg.) in Uganda |
| title_full_unstemmed |
Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Mull. Arg.) in Uganda |
| title_sort |
diversity of begomoviruses associated with mosaic disease of cultivated cassava (manihot esculenta crantz) and its wild relative (manihot glaziovii mull. arg.) in uganda |
| publisher |
Microbiology Society |
| publishDate |
2008-07-01 |
| url |
https://hdl.handle.net/10568/90781 https://doi.org/10.1099/vir.0.83637-0 |
| work_keys_str_mv |
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| _version_ |
1779053026212839424 |