Cloning of the bovine interleukin-3-encoding cDNA. (Colony-stimulating factor: Cytokine: PCR: Neuclotide sequence: RACE)
Interleukin-3(IL-3) is one of the cytokines that act during the early and late stages of blood cell formation. To enable the study of the role of IL-3 in bovine haemopoietic stem cell differentiation, the polymerase chain reaction was used to amplify an IL-3 cDNA from first-strand cDNAs prepared from RNA isolated from 4- and 5-hour concanavalin-A-stimulated peripheral blood lymphocytes (PBL) from N'Dama cattle. An analysis of the cDNA sequence reveals that it contains a 432-nucleotide (nt) open reading frame which codes for 144 amino acids (aa). Cleavage of the putative signal peptide consisting of the first 17 aa yields the mature form of the protein (14.5 kDa). Comparisons of the bovine IL-3 sequence with the sheep, human and mouse IL-3 sequences show that the bovine sequence shares 90.7.55.8 and 51.9 percent nt identity, respectively, in the coding region, and 85.4.35 and 27.7 percent aa identity respectively.
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Format: | Journal Article biblioteca |
Language: | English |
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Elsevier
1995-09
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Subjects: | cattle, dna, cytology, blood cells, molecular cloning, nucleotide sequence, genetics, |
Online Access: | https://hdl.handle.net/10568/28649 https://doi.org/10.1016/0378-1119(95)00359-E |
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dig-cgspace-10568-286492023-12-08T19:36:04Z Cloning of the bovine interleukin-3-encoding cDNA. (Colony-stimulating factor: Cytokine: PCR: Neuclotide sequence: RACE) Mwangi, S.M. Logan-Henfrey, L.L. McInnes, C. Mertens, B. cattle dna cytology blood cells molecular cloning nucleotide sequence genetics Interleukin-3(IL-3) is one of the cytokines that act during the early and late stages of blood cell formation. To enable the study of the role of IL-3 in bovine haemopoietic stem cell differentiation, the polymerase chain reaction was used to amplify an IL-3 cDNA from first-strand cDNAs prepared from RNA isolated from 4- and 5-hour concanavalin-A-stimulated peripheral blood lymphocytes (PBL) from N'Dama cattle. An analysis of the cDNA sequence reveals that it contains a 432-nucleotide (nt) open reading frame which codes for 144 amino acids (aa). Cleavage of the putative signal peptide consisting of the first 17 aa yields the mature form of the protein (14.5 kDa). Comparisons of the bovine IL-3 sequence with the sheep, human and mouse IL-3 sequences show that the bovine sequence shares 90.7.55.8 and 51.9 percent nt identity, respectively, in the coding region, and 85.4.35 and 27.7 percent aa identity respectively. 1995-09 2013-05-06T07:01:05Z 2013-05-06T07:01:05Z Journal Article Gene;162: 309-312 0378-1119 https://hdl.handle.net/10568/28649 https://doi.org/10.1016/0378-1119(95)00359-E en Copyrighted; all rights reserved Limited Access p. 309-312 Elsevier Gene |
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cattle dna cytology blood cells molecular cloning nucleotide sequence genetics cattle dna cytology blood cells molecular cloning nucleotide sequence genetics |
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cattle dna cytology blood cells molecular cloning nucleotide sequence genetics cattle dna cytology blood cells molecular cloning nucleotide sequence genetics Mwangi, S.M. Logan-Henfrey, L.L. McInnes, C. Mertens, B. Cloning of the bovine interleukin-3-encoding cDNA. (Colony-stimulating factor: Cytokine: PCR: Neuclotide sequence: RACE) |
description |
Interleukin-3(IL-3) is one of the cytokines that act during the early and late stages of blood cell formation. To enable the study of the role of IL-3 in bovine haemopoietic stem cell differentiation, the polymerase chain reaction was used to amplify an IL-3 cDNA from first-strand cDNAs prepared from RNA isolated from 4- and 5-hour concanavalin-A-stimulated peripheral blood lymphocytes (PBL) from N'Dama cattle. An analysis of the cDNA sequence reveals that it contains a 432-nucleotide (nt) open reading frame which codes for 144 amino acids (aa). Cleavage of the putative signal peptide consisting of the first 17 aa yields the mature form of the protein (14.5 kDa). Comparisons of the bovine IL-3 sequence with the sheep, human and mouse IL-3 sequences show that the bovine sequence shares 90.7.55.8 and 51.9 percent nt identity, respectively, in the coding region, and 85.4.35 and 27.7 percent aa identity respectively. |
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Journal Article |
topic_facet |
cattle dna cytology blood cells molecular cloning nucleotide sequence genetics |
author |
Mwangi, S.M. Logan-Henfrey, L.L. McInnes, C. Mertens, B. |
author_facet |
Mwangi, S.M. Logan-Henfrey, L.L. McInnes, C. Mertens, B. |
author_sort |
Mwangi, S.M. |
title |
Cloning of the bovine interleukin-3-encoding cDNA. (Colony-stimulating factor: Cytokine: PCR: Neuclotide sequence: RACE) |
title_short |
Cloning of the bovine interleukin-3-encoding cDNA. (Colony-stimulating factor: Cytokine: PCR: Neuclotide sequence: RACE) |
title_full |
Cloning of the bovine interleukin-3-encoding cDNA. (Colony-stimulating factor: Cytokine: PCR: Neuclotide sequence: RACE) |
title_fullStr |
Cloning of the bovine interleukin-3-encoding cDNA. (Colony-stimulating factor: Cytokine: PCR: Neuclotide sequence: RACE) |
title_full_unstemmed |
Cloning of the bovine interleukin-3-encoding cDNA. (Colony-stimulating factor: Cytokine: PCR: Neuclotide sequence: RACE) |
title_sort |
cloning of the bovine interleukin-3-encoding cdna. (colony-stimulating factor: cytokine: pcr: neuclotide sequence: race) |
publisher |
Elsevier |
publishDate |
1995-09 |
url |
https://hdl.handle.net/10568/28649 https://doi.org/10.1016/0378-1119(95)00359-E |
work_keys_str_mv |
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