A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae)
Rhodnius prolixus is the main vector of Chagas disease in Venezuela, where it is found colonising rural housing consisting of unplastered adobe walls with palm and/or metal roofs. Vector control failure in Venezuela may be due to the invasion of houses by silvatic populations of R. prolixus found in palms. As part of a study to determine if domestic and silvatic populations of R. prolixus are isolated, thus clarifying the role of silvatic populations in maintaining house infestations, we constructed three partial genomic microsatellite libraries. A panel of ten dinucleotide polymorphic microsatellite markers was selected for genotyping. Allele numbers per locus ranged from three to twelve, with observed and expected heterozygosity ranging from 0.26 to 0.55 and 0.32 to 0.66. The microsatellite markers presented here will contribute to the control of Chagas disease in Venezuela and Colombia through the provision of population information that may allow the design of improved control strategies.
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Elsevier
2009-03
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Subjects: | genetics, animal diseases, |
Online Access: | https://hdl.handle.net/10568/2166 https://doi.org/10.1016/j.meegid.2008.10.017 |
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dig-cgspace-10568-21662023-12-08T19:36:04Z A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae) Fitzpatrick, S. Watts, P.C. Feliciangeli, M.D. Miles, M.A. Kemp, Stephen J. genetics animal diseases Rhodnius prolixus is the main vector of Chagas disease in Venezuela, where it is found colonising rural housing consisting of unplastered adobe walls with palm and/or metal roofs. Vector control failure in Venezuela may be due to the invasion of houses by silvatic populations of R. prolixus found in palms. As part of a study to determine if domestic and silvatic populations of R. prolixus are isolated, thus clarifying the role of silvatic populations in maintaining house infestations, we constructed three partial genomic microsatellite libraries. A panel of ten dinucleotide polymorphic microsatellite markers was selected for genotyping. Allele numbers per locus ranged from three to twelve, with observed and expected heterozygosity ranging from 0.26 to 0.55 and 0.32 to 0.66. The microsatellite markers presented here will contribute to the control of Chagas disease in Venezuela and Colombia through the provision of population information that may allow the design of improved control strategies. 2009-03 2010-08-04T13:28:06Z 2010-08-04T13:28:06Z Journal Article Fitzpatrick, S.; Watts, P.C.; Feliciangeli, M.D.; Miles, M.A.; Kemp, S.J. 2009. A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae). Infection Genetics and Evolution 9(2):206-209. 1567-1348 https://hdl.handle.net/10568/2166 https://doi.org/10.1016/j.meegid.2008.10.017 en CC-BY-3.0 Open Access p. 206-209 Elsevier Infection, Genetics and Evolution |
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genetics animal diseases genetics animal diseases Fitzpatrick, S. Watts, P.C. Feliciangeli, M.D. Miles, M.A. Kemp, Stephen J. A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae) |
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Rhodnius prolixus is the main vector of Chagas disease in Venezuela, where it is found colonising rural housing consisting of unplastered adobe walls with palm and/or metal roofs. Vector control failure in Venezuela may be due to the invasion of houses by silvatic populations of R. prolixus found in palms. As part of a study to determine if domestic and silvatic populations of R. prolixus are isolated, thus clarifying the role of silvatic populations in maintaining house infestations, we constructed three partial genomic microsatellite libraries. A panel of ten dinucleotide polymorphic microsatellite markers was selected for genotyping. Allele numbers per locus ranged from three to twelve, with observed and expected heterozygosity ranging from 0.26 to 0.55 and 0.32 to 0.66. The microsatellite markers presented here will contribute to the control of Chagas disease in Venezuela and Colombia through the provision of population information that may allow the design of improved control strategies. |
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Journal Article |
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genetics animal diseases |
author |
Fitzpatrick, S. Watts, P.C. Feliciangeli, M.D. Miles, M.A. Kemp, Stephen J. |
author_facet |
Fitzpatrick, S. Watts, P.C. Feliciangeli, M.D. Miles, M.A. Kemp, Stephen J. |
author_sort |
Fitzpatrick, S. |
title |
A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae) |
title_short |
A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae) |
title_full |
A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae) |
title_fullStr |
A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae) |
title_full_unstemmed |
A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae) |
title_sort |
panel of ten microsatellite loci for the chagas disease vector rhodnius prolixus (hemiptera: reduviidae) |
publisher |
Elsevier |
publishDate |
2009-03 |
url |
https://hdl.handle.net/10568/2166 https://doi.org/10.1016/j.meegid.2008.10.017 |
work_keys_str_mv |
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