A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae)

Rhodnius prolixus is the main vector of Chagas disease in Venezuela, where it is found colonising rural housing consisting of unplastered adobe walls with palm and/or metal roofs. Vector control failure in Venezuela may be due to the invasion of houses by silvatic populations of R. prolixus found in palms. As part of a study to determine if domestic and silvatic populations of R. prolixus are isolated, thus clarifying the role of silvatic populations in maintaining house infestations, we constructed three partial genomic microsatellite libraries. A panel of ten dinucleotide polymorphic microsatellite markers was selected for genotyping. Allele numbers per locus ranged from three to twelve, with observed and expected heterozygosity ranging from 0.26 to 0.55 and 0.32 to 0.66. The microsatellite markers presented here will contribute to the control of Chagas disease in Venezuela and Colombia through the provision of population information that may allow the design of improved control strategies.

Saved in:
Bibliographic Details
Main Authors: Fitzpatrick, S., Watts, P.C., Feliciangeli, M.D., Miles, M.A., Kemp, Stephen J.
Format: Journal Article biblioteca
Language:English
Published: Elsevier 2009-03
Subjects:genetics, animal diseases,
Online Access:https://hdl.handle.net/10568/2166
https://doi.org/10.1016/j.meegid.2008.10.017
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-cgspace-10568-2166
record_format koha
spelling dig-cgspace-10568-21662023-12-08T19:36:04Z A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae) Fitzpatrick, S. Watts, P.C. Feliciangeli, M.D. Miles, M.A. Kemp, Stephen J. genetics animal diseases Rhodnius prolixus is the main vector of Chagas disease in Venezuela, where it is found colonising rural housing consisting of unplastered adobe walls with palm and/or metal roofs. Vector control failure in Venezuela may be due to the invasion of houses by silvatic populations of R. prolixus found in palms. As part of a study to determine if domestic and silvatic populations of R. prolixus are isolated, thus clarifying the role of silvatic populations in maintaining house infestations, we constructed three partial genomic microsatellite libraries. A panel of ten dinucleotide polymorphic microsatellite markers was selected for genotyping. Allele numbers per locus ranged from three to twelve, with observed and expected heterozygosity ranging from 0.26 to 0.55 and 0.32 to 0.66. The microsatellite markers presented here will contribute to the control of Chagas disease in Venezuela and Colombia through the provision of population information that may allow the design of improved control strategies. 2009-03 2010-08-04T13:28:06Z 2010-08-04T13:28:06Z Journal Article Fitzpatrick, S.; Watts, P.C.; Feliciangeli, M.D.; Miles, M.A.; Kemp, S.J. 2009. A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae). Infection Genetics and Evolution 9(2):206-209. 1567-1348 https://hdl.handle.net/10568/2166 https://doi.org/10.1016/j.meegid.2008.10.017 en CC-BY-3.0 Open Access p. 206-209 Elsevier Infection, Genetics and Evolution
institution CGIAR
collection DSpace
country Francia
countrycode FR
component Bibliográfico
access En linea
databasecode dig-cgspace
tag biblioteca
region Europa del Oeste
libraryname Biblioteca del CGIAR
language English
topic genetics
animal diseases
genetics
animal diseases
spellingShingle genetics
animal diseases
genetics
animal diseases
Fitzpatrick, S.
Watts, P.C.
Feliciangeli, M.D.
Miles, M.A.
Kemp, Stephen J.
A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae)
description Rhodnius prolixus is the main vector of Chagas disease in Venezuela, where it is found colonising rural housing consisting of unplastered adobe walls with palm and/or metal roofs. Vector control failure in Venezuela may be due to the invasion of houses by silvatic populations of R. prolixus found in palms. As part of a study to determine if domestic and silvatic populations of R. prolixus are isolated, thus clarifying the role of silvatic populations in maintaining house infestations, we constructed three partial genomic microsatellite libraries. A panel of ten dinucleotide polymorphic microsatellite markers was selected for genotyping. Allele numbers per locus ranged from three to twelve, with observed and expected heterozygosity ranging from 0.26 to 0.55 and 0.32 to 0.66. The microsatellite markers presented here will contribute to the control of Chagas disease in Venezuela and Colombia through the provision of population information that may allow the design of improved control strategies.
format Journal Article
topic_facet genetics
animal diseases
author Fitzpatrick, S.
Watts, P.C.
Feliciangeli, M.D.
Miles, M.A.
Kemp, Stephen J.
author_facet Fitzpatrick, S.
Watts, P.C.
Feliciangeli, M.D.
Miles, M.A.
Kemp, Stephen J.
author_sort Fitzpatrick, S.
title A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae)
title_short A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae)
title_full A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae)
title_fullStr A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae)
title_full_unstemmed A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae)
title_sort panel of ten microsatellite loci for the chagas disease vector rhodnius prolixus (hemiptera: reduviidae)
publisher Elsevier
publishDate 2009-03
url https://hdl.handle.net/10568/2166
https://doi.org/10.1016/j.meegid.2008.10.017
work_keys_str_mv AT fitzpatricks apaneloftenmicrosatellitelociforthechagasdiseasevectorrhodniusprolixushemipterareduviidae
AT wattspc apaneloftenmicrosatellitelociforthechagasdiseasevectorrhodniusprolixushemipterareduviidae
AT feliciangelimd apaneloftenmicrosatellitelociforthechagasdiseasevectorrhodniusprolixushemipterareduviidae
AT milesma apaneloftenmicrosatellitelociforthechagasdiseasevectorrhodniusprolixushemipterareduviidae
AT kempstephenj apaneloftenmicrosatellitelociforthechagasdiseasevectorrhodniusprolixushemipterareduviidae
AT fitzpatricks paneloftenmicrosatellitelociforthechagasdiseasevectorrhodniusprolixushemipterareduviidae
AT wattspc paneloftenmicrosatellitelociforthechagasdiseasevectorrhodniusprolixushemipterareduviidae
AT feliciangelimd paneloftenmicrosatellitelociforthechagasdiseasevectorrhodniusprolixushemipterareduviidae
AT milesma paneloftenmicrosatellitelociforthechagasdiseasevectorrhodniusprolixushemipterareduviidae
AT kempstephenj paneloftenmicrosatellitelociforthechagasdiseasevectorrhodniusprolixushemipterareduviidae
_version_ 1787230567880720384