Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions
Desmanthus virgatus is a perennial forage legume used as a livestock feed source. Seeds of this species are physically dormant and thus, propagation is difficult. The dormancy also impacts the routine viability monitoring of genebank accessions. The present study investigated different methods to break dormancy in seeds collected from various locations at different times. The result showed that most pre-treatments significantly increased germination percentage. Accessions treated with concentrated sulphuric acid (25 or 30 minutes) followed by sandpaper scarification showed the highest proportion of normal seedlings (94.6 and 96%). Boiling water (2.3 or 3 minutes) was also effective in all accessions except for accession 331. However, accessions treated with hot water (3, 5 or 8 minutes) had a considerable proportion of hard seeds. The proportion of dead/abnormal seedlings was high in seeds treated with a hot wire in all accessions, ranging from 27.5 (accession 15489) to 75.5% (accession 16). The untreated seeds remained hard at the final germination count. We conclude that it is important to use concentrated sulphuric acid and mechanical scarification with sandpaper to break the dormancy for the species to enhance germination, obtain uniform field stands and make wise decisions during storage.
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Format: | Journal Article biblioteca |
Language: | English |
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2023-10-16
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Subjects: | Fabaceae, genebank, hedge lucerne, physical dormancy, scarification, seed longevity, viability, |
Online Access: | https://hdl.handle.net/10568/135256 https://doi.org/10.15258/sst.2023.51.3.12 |
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dig-cgspace-10568-1352562023-12-16T04:21:18Z Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions Olbana, Tamiru Muchugi, Alice Woldemariam, Yeshi Hay, Fiona R. Ndiwa, Nicholas Jones, Christopher S. Fabaceae genebank hedge lucerne physical dormancy scarification seed longevity viability Desmanthus virgatus is a perennial forage legume used as a livestock feed source. Seeds of this species are physically dormant and thus, propagation is difficult. The dormancy also impacts the routine viability monitoring of genebank accessions. The present study investigated different methods to break dormancy in seeds collected from various locations at different times. The result showed that most pre-treatments significantly increased germination percentage. Accessions treated with concentrated sulphuric acid (25 or 30 minutes) followed by sandpaper scarification showed the highest proportion of normal seedlings (94.6 and 96%). Boiling water (2.3 or 3 minutes) was also effective in all accessions except for accession 331. However, accessions treated with hot water (3, 5 or 8 minutes) had a considerable proportion of hard seeds. The proportion of dead/abnormal seedlings was high in seeds treated with a hot wire in all accessions, ranging from 27.5 (accession 15489) to 75.5% (accession 16). The untreated seeds remained hard at the final germination count. We conclude that it is important to use concentrated sulphuric acid and mechanical scarification with sandpaper to break the dormancy for the species to enhance germination, obtain uniform field stands and make wise decisions during storage. 2023-10-16 2023-12-12T08:03:47Z 2023-12-12T08:03:47Z Journal Article Olbana, T., Muchugi, A., Woldemariam, Y., Hay, F.R., Ndiwa, N. and Jones, C.S. 2023. Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions. Seed Science and Technology 51(3):421-434. 1819-5717 https://hdl.handle.net/10568/135256 https://doi.org/10.15258/sst.2023.51.3.12 en CC-BY-NC-4.0 Open Access Seed Science and Technology |
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Fabaceae genebank hedge lucerne physical dormancy scarification seed longevity viability Fabaceae genebank hedge lucerne physical dormancy scarification seed longevity viability |
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Fabaceae genebank hedge lucerne physical dormancy scarification seed longevity viability Fabaceae genebank hedge lucerne physical dormancy scarification seed longevity viability Olbana, Tamiru Muchugi, Alice Woldemariam, Yeshi Hay, Fiona R. Ndiwa, Nicholas Jones, Christopher S. Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions |
description |
Desmanthus virgatus is a perennial forage legume used as a livestock feed source. Seeds of this species are physically dormant and thus, propagation is difficult. The dormancy also impacts the routine viability monitoring of genebank accessions. The present study investigated different methods to break dormancy in seeds collected from various locations at different times. The result showed that most pre-treatments significantly increased germination percentage. Accessions treated with concentrated sulphuric acid (25 or 30 minutes) followed by sandpaper scarification showed the highest proportion of normal seedlings (94.6 and 96%). Boiling water (2.3 or 3 minutes) was also effective in all accessions except for accession 331. However, accessions treated with hot water (3, 5 or 8 minutes) had a considerable proportion of hard seeds. The proportion of dead/abnormal seedlings was high in seeds treated with a hot wire in all accessions, ranging from 27.5 (accession 15489) to 75.5% (accession 16). The untreated seeds remained hard at the final germination count. We conclude that it is important to use concentrated sulphuric acid and mechanical scarification with sandpaper to break the dormancy for the species to enhance germination, obtain uniform field stands and make wise decisions during storage. |
format |
Journal Article |
topic_facet |
Fabaceae genebank hedge lucerne physical dormancy scarification seed longevity viability |
author |
Olbana, Tamiru Muchugi, Alice Woldemariam, Yeshi Hay, Fiona R. Ndiwa, Nicholas Jones, Christopher S. |
author_facet |
Olbana, Tamiru Muchugi, Alice Woldemariam, Yeshi Hay, Fiona R. Ndiwa, Nicholas Jones, Christopher S. |
author_sort |
Olbana, Tamiru |
title |
Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions |
title_short |
Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions |
title_full |
Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions |
title_fullStr |
Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions |
title_full_unstemmed |
Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions |
title_sort |
overcoming dormancy in desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions |
publishDate |
2023-10-16 |
url |
https://hdl.handle.net/10568/135256 https://doi.org/10.15258/sst.2023.51.3.12 |
work_keys_str_mv |
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1787228892783706112 |