Rare instances of haploid inducer DNA in potato dihaploids and ploidy-dependent genome instability
In cultivated tetraploid potato (Solanum tuberosum), reduction to diploidy (dihaploidy) allows for hybridization to diploids and introgression breeding and may facilitate the production of inbreds. Pollination with haploid inducers (HIs) yields maternal dihaploids, as well as triploid and tetraploid hybrids. Dihaploids may result from parthenogenesis, entailing the development of embryos from unfertilized eggs, or genome elimination, entailing missegregation and the loss of paternal chromosomes. A sign of genome elimination is the occasional persistence of HI DNA in some dihaploids. We characterized the genomes of 919 putative dihaploids and 134 hybrids produced by pollinating tetraploid clones with three HIs: IVP35, IVP101, and PL-4. Whole-chromosome or segmental aneuploidy was observed in 76 dihaploids, with karyotypes ranging from 2n = 2x − 1 = 23 to 2n = 2x + 3 = 27. Of the additional chromosomes in 74 aneuploids, 66 were from the non-inducer parent and 8 from the inducer parent. Overall, we detected full or partial chromosomes from the HI parent in 0.87% of the dihaploids, irrespective of parental genotypes. Chromosomal breaks commonly affected the paternal genome in the dihaploid and tetraploid progeny, but not in the triploid progeny, correlating instability to sperm ploidy and to haploid induction. The residual HI DNA discovered in the progeny is consistent with genome elimination as the mechanism of haploid induction.
Main Authors: | , , , , , , , , |
---|---|
Format: | Journal Article biblioteca |
Language: | English |
Published: |
Oxford University Press
2021-08-13
|
Subjects: | potatoes, dna, genomes, haploidy, |
Online Access: | https://hdl.handle.net/10568/115535 https://doi.org/10.1093/plcell/koab100 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cgspace-10568-115535 |
---|---|
record_format |
koha |
spelling |
dig-cgspace-10568-1155352023-12-08T19:36:04Z Rare instances of haploid inducer DNA in potato dihaploids and ploidy-dependent genome instability Amundson, K.R. Ordoñez, B. Santayana, M. Nganga, M.L. Henry, I.M. Bonierbale, Merideth W. Khan, A. Tan, E.H. Comai, L. potatoes dna genomes haploidy In cultivated tetraploid potato (Solanum tuberosum), reduction to diploidy (dihaploidy) allows for hybridization to diploids and introgression breeding and may facilitate the production of inbreds. Pollination with haploid inducers (HIs) yields maternal dihaploids, as well as triploid and tetraploid hybrids. Dihaploids may result from parthenogenesis, entailing the development of embryos from unfertilized eggs, or genome elimination, entailing missegregation and the loss of paternal chromosomes. A sign of genome elimination is the occasional persistence of HI DNA in some dihaploids. We characterized the genomes of 919 putative dihaploids and 134 hybrids produced by pollinating tetraploid clones with three HIs: IVP35, IVP101, and PL-4. Whole-chromosome or segmental aneuploidy was observed in 76 dihaploids, with karyotypes ranging from 2n = 2x − 1 = 23 to 2n = 2x + 3 = 27. Of the additional chromosomes in 74 aneuploids, 66 were from the non-inducer parent and 8 from the inducer parent. Overall, we detected full or partial chromosomes from the HI parent in 0.87% of the dihaploids, irrespective of parental genotypes. Chromosomal breaks commonly affected the paternal genome in the dihaploid and tetraploid progeny, but not in the triploid progeny, correlating instability to sperm ploidy and to haploid induction. The residual HI DNA discovered in the progeny is consistent with genome elimination as the mechanism of haploid induction. 2021-08-13 2021-10-20T04:55:30Z 2021-10-20T04:55:30Z Journal Article Amundson, K. R.; Ordoñez, B.; Santayana, M.; Nganga, M. L.; Henry, I. M.; Bonierbale, M.; Khan, A.; Tan, E. H.; Comai, L. 2021. Rare instances of haploid inducer DNA in potato dihaploids and ploidy-dependent genome instability. The Plant Cell. ISSN 1532-298X. 33(7), 2149–2163. 39 p 1532-298X https://hdl.handle.net/10568/115535 https://doi.org/10.1093/plcell/koab100 en CC-BY-4.0 Open Access 2149-2163 Oxford University Press The Plant Cell |
institution |
CGIAR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cgspace |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CGIAR |
language |
English |
topic |
potatoes dna genomes haploidy potatoes dna genomes haploidy |
spellingShingle |
potatoes dna genomes haploidy potatoes dna genomes haploidy Amundson, K.R. Ordoñez, B. Santayana, M. Nganga, M.L. Henry, I.M. Bonierbale, Merideth W. Khan, A. Tan, E.H. Comai, L. Rare instances of haploid inducer DNA in potato dihaploids and ploidy-dependent genome instability |
description |
In cultivated tetraploid potato (Solanum tuberosum), reduction to diploidy (dihaploidy) allows for hybridization to diploids and introgression breeding and may facilitate the production of inbreds. Pollination with haploid inducers (HIs) yields maternal dihaploids, as well as triploid and tetraploid hybrids. Dihaploids may result from parthenogenesis, entailing the development of embryos from unfertilized eggs, or genome elimination, entailing missegregation and the loss of paternal chromosomes. A sign of genome elimination is the occasional persistence of HI DNA in some dihaploids. We characterized the genomes of 919 putative dihaploids and 134 hybrids produced by pollinating tetraploid clones with three HIs: IVP35, IVP101, and PL-4. Whole-chromosome or segmental aneuploidy was observed in 76 dihaploids, with karyotypes ranging from 2n = 2x − 1 = 23 to 2n = 2x + 3 = 27. Of the additional chromosomes in 74 aneuploids, 66 were from the non-inducer parent and 8 from the inducer parent. Overall, we detected full or partial chromosomes from the HI parent in 0.87% of the dihaploids, irrespective of parental genotypes. Chromosomal breaks commonly affected the paternal genome in the dihaploid and tetraploid progeny, but not in the triploid progeny, correlating instability to sperm ploidy and to haploid induction. The residual HI DNA discovered in the progeny is consistent with genome elimination as the mechanism of haploid induction. |
format |
Journal Article |
topic_facet |
potatoes dna genomes haploidy |
author |
Amundson, K.R. Ordoñez, B. Santayana, M. Nganga, M.L. Henry, I.M. Bonierbale, Merideth W. Khan, A. Tan, E.H. Comai, L. |
author_facet |
Amundson, K.R. Ordoñez, B. Santayana, M. Nganga, M.L. Henry, I.M. Bonierbale, Merideth W. Khan, A. Tan, E.H. Comai, L. |
author_sort |
Amundson, K.R. |
title |
Rare instances of haploid inducer DNA in potato dihaploids and ploidy-dependent genome instability |
title_short |
Rare instances of haploid inducer DNA in potato dihaploids and ploidy-dependent genome instability |
title_full |
Rare instances of haploid inducer DNA in potato dihaploids and ploidy-dependent genome instability |
title_fullStr |
Rare instances of haploid inducer DNA in potato dihaploids and ploidy-dependent genome instability |
title_full_unstemmed |
Rare instances of haploid inducer DNA in potato dihaploids and ploidy-dependent genome instability |
title_sort |
rare instances of haploid inducer dna in potato dihaploids and ploidy-dependent genome instability |
publisher |
Oxford University Press |
publishDate |
2021-08-13 |
url |
https://hdl.handle.net/10568/115535 https://doi.org/10.1093/plcell/koab100 |
work_keys_str_mv |
AT amundsonkr rareinstancesofhaploidinducerdnainpotatodihaploidsandploidydependentgenomeinstability AT ordonezb rareinstancesofhaploidinducerdnainpotatodihaploidsandploidydependentgenomeinstability AT santayanam rareinstancesofhaploidinducerdnainpotatodihaploidsandploidydependentgenomeinstability AT ngangaml rareinstancesofhaploidinducerdnainpotatodihaploidsandploidydependentgenomeinstability AT henryim rareinstancesofhaploidinducerdnainpotatodihaploidsandploidydependentgenomeinstability AT bonierbalemeridethw rareinstancesofhaploidinducerdnainpotatodihaploidsandploidydependentgenomeinstability AT khana rareinstancesofhaploidinducerdnainpotatodihaploidsandploidydependentgenomeinstability AT taneh rareinstancesofhaploidinducerdnainpotatodihaploidsandploidydependentgenomeinstability AT comail rareinstancesofhaploidinducerdnainpotatodihaploidsandploidydependentgenomeinstability |
_version_ |
1787231418302070784 |