Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica)
Host-specific interactions can maintain genetic and phenotypic diversity in parasites that attack multiple host species. Host diversity, in turn, may promote parasite diversity by selection for genetic divergence or plastic responses to host type. The parasitic weed purple witchweed [Striga hermonthica (Delile) Benth.] causes devastating crop losses in sub-Saharan Africa and is capable of infesting a wide range of grass hosts. Despite some evidence for host adaptation and host-by-Striga genotype interactions, little is known about intraspecific Striga genomic diversity. Here we present a study of transcriptomic diversity in populations of S. hermonthica growing on different hosts (maize [Zea mays L.] vs. grain sorghum [Sorghum bicolor (L.) Moench]). We examined gene expression variation and differences in allelic frequency in expressed genes of aboveground tissues from populations in western Nigeria parasitizing each host. Despite low levels of host-based genome-wide differentiation, we identified a set of parasite transcripts specifically associated with each host. Parasite genes in several different functional categories implicated as important in host–parasite interactions differed in expression level and allele on different hosts, including genes involved in nutrient transport, defense and pathogenesis, and plant hormone response. Overall, we provide a set of candidate transcripts that demonstrate host-specific interactions in vegetative tissues of the emerged parasite S. hermonthica. Our study shows how signals of host-specific processes can be detected aboveground, expanding the focus of host–parasite interactions beyond the haustorial connection.
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Format: | Journal Article biblioteca |
Language: | English |
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Cambridge University Press
2019-07
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Subjects: | allele frequency, gene expression, host parasite relations, parasitic plant, rna sequence, maize, striga hermonthica, |
Online Access: | https://hdl.handle.net/10568/109247 https://doi.org/10.1017/wsc.2019.20 |
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dig-cgspace-10568-1092472023-12-08T19:36:04Z Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica) López, L. Bellis, E.S. Wafula, E. Hearne, S. Honaas, L. Ralph, P.E. Timko, M.P. Unachukwu, N.N. dePamphilis, C.W. Lasky, J.R. allele frequency gene expression host parasite relations parasitic plant rna sequence maize striga hermonthica Host-specific interactions can maintain genetic and phenotypic diversity in parasites that attack multiple host species. Host diversity, in turn, may promote parasite diversity by selection for genetic divergence or plastic responses to host type. The parasitic weed purple witchweed [Striga hermonthica (Delile) Benth.] causes devastating crop losses in sub-Saharan Africa and is capable of infesting a wide range of grass hosts. Despite some evidence for host adaptation and host-by-Striga genotype interactions, little is known about intraspecific Striga genomic diversity. Here we present a study of transcriptomic diversity in populations of S. hermonthica growing on different hosts (maize [Zea mays L.] vs. grain sorghum [Sorghum bicolor (L.) Moench]). We examined gene expression variation and differences in allelic frequency in expressed genes of aboveground tissues from populations in western Nigeria parasitizing each host. Despite low levels of host-based genome-wide differentiation, we identified a set of parasite transcripts specifically associated with each host. Parasite genes in several different functional categories implicated as important in host–parasite interactions differed in expression level and allele on different hosts, including genes involved in nutrient transport, defense and pathogenesis, and plant hormone response. Overall, we provide a set of candidate transcripts that demonstrate host-specific interactions in vegetative tissues of the emerged parasite S. hermonthica. Our study shows how signals of host-specific processes can be detected aboveground, expanding the focus of host–parasite interactions beyond the haustorial connection. 2019-07 2020-09-07T11:31:32Z 2020-09-07T11:31:32Z Journal Article Lopez, L., Bellis, E.S., Wafula, E., Hearne, S.J., Honaas, L., Ralph, P.E., ... & Lasky, J.R. (2019). Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica). Weed Science, 67(4), 397-411. 0043-1745 https://hdl.handle.net/10568/109247 https://doi.org/10.1017/wsc.2019.20 en Copyrighted; all rights reserved Limited Access 397-411 Cambridge University Press Weed Science |
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allele frequency gene expression host parasite relations parasitic plant rna sequence maize striga hermonthica allele frequency gene expression host parasite relations parasitic plant rna sequence maize striga hermonthica |
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allele frequency gene expression host parasite relations parasitic plant rna sequence maize striga hermonthica allele frequency gene expression host parasite relations parasitic plant rna sequence maize striga hermonthica López, L. Bellis, E.S. Wafula, E. Hearne, S. Honaas, L. Ralph, P.E. Timko, M.P. Unachukwu, N.N. dePamphilis, C.W. Lasky, J.R. Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica) |
description |
Host-specific interactions can maintain genetic and phenotypic diversity in parasites that attack multiple host species. Host diversity, in turn, may promote parasite diversity by selection for genetic divergence or plastic responses to host type. The parasitic weed purple witchweed [Striga hermonthica (Delile) Benth.] causes devastating crop losses in sub-Saharan Africa and is capable of infesting a wide range of grass hosts. Despite some evidence for host adaptation and host-by-Striga genotype interactions, little is known about intraspecific Striga genomic diversity. Here we present a study of transcriptomic diversity in populations of S. hermonthica growing on different hosts (maize [Zea mays L.] vs. grain sorghum [Sorghum bicolor (L.) Moench]). We examined gene expression variation and differences in allelic frequency in expressed genes of aboveground tissues from populations in western Nigeria parasitizing each host. Despite low levels of host-based genome-wide differentiation, we identified a set of parasite transcripts specifically associated with each host. Parasite genes in several different functional categories implicated as important in host–parasite interactions differed in expression level and allele on different hosts, including genes involved in nutrient transport, defense and pathogenesis, and plant hormone response. Overall, we provide a set of candidate transcripts that demonstrate host-specific interactions in vegetative tissues of the emerged parasite S. hermonthica. Our study shows how signals of host-specific processes can be detected aboveground, expanding the focus of host–parasite interactions beyond the haustorial connection. |
format |
Journal Article |
topic_facet |
allele frequency gene expression host parasite relations parasitic plant rna sequence maize striga hermonthica |
author |
López, L. Bellis, E.S. Wafula, E. Hearne, S. Honaas, L. Ralph, P.E. Timko, M.P. Unachukwu, N.N. dePamphilis, C.W. Lasky, J.R. |
author_facet |
López, L. Bellis, E.S. Wafula, E. Hearne, S. Honaas, L. Ralph, P.E. Timko, M.P. Unachukwu, N.N. dePamphilis, C.W. Lasky, J.R. |
author_sort |
López, L. |
title |
Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica) |
title_short |
Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica) |
title_full |
Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica) |
title_fullStr |
Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica) |
title_full_unstemmed |
Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica) |
title_sort |
transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (striga hermonthica) |
publisher |
Cambridge University Press |
publishDate |
2019-07 |
url |
https://hdl.handle.net/10568/109247 https://doi.org/10.1017/wsc.2019.20 |
work_keys_str_mv |
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