Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum
Diadema setosum (Leske, 1778), is one of the common echinoids widely distributed in the Indo-West Pacific Ocean, where it occurs from the Red Sea, Persian Gulf and the east coast of Africa to Japan, Australia and Malaysia. To investigate the developmental basis of morphological changes in embryos and larvae, we documented the ontogeny of D. setosum in a controlled laboratory condition at the Institute of Bioscience, Universiti Putra Malaysia, during July–September, 2012. Matured gametes were obtained from the adult individuals and the eggs fertilized at limited sperm concentration (10^-5 dilution). The obtained embryos were then reared at 24-25°C. First cleavage (2-cell), 4-cell, 8-cell, 16-cell, 32-cell and multi-cell (Morulla) stages were attained at 01.20, 02.14, 02.44, 03.09, 03.32 and 03.54 h after fertilization, respectively. Blastulae with a mean length of 111.47±1.88 µm (mean±SD) hatched 08.45 h after sperm entry. Gastrula formed 16.36 h post-fertilization and the archenteron extended constantly, while the ectodermal red-pigmented cells migrated synchronously to the apical plate. The pluteus larva started to feed unicellular algae (Chaetoceros calcitrans) in 2 d, grew continuously, and finally attained metamorphic competence within 35 d after fertilization. Induction of metamorphosis took approximately 1 h 30 min from attachment on the substratum to the complete resorption of larval tissues and the development of complete juvenile structure with adult spines, extended tubefeet and well-developed pedicellaria, the whole event usually took place within 1 d post-settlement. The newly formed juvenile (473.16 ± 6.96 µm, n=30) with a complete adult structure then grew on coralline algae to 3-month old juvenile, which represents the "sea urchin seed" for stocking in grow-out culture. This study represents the first successful investigation on embryonic, larval and early juvenile development of D. setosum. The findings would greatly be helpful towards the development of breeding and seed production techniques for aquaculture of sea urchins.
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2015
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Assuntos: | Aquaculture, Biology, Fisheries, Sea urchin, Diadema setosum, Embryo, Larva, Juvenile, Development, Pulau Pangkor, aquaculture, Pacific Ocean, Red Sea, Persian Gulf, Africa, Japan, Australia, Malaysia, |
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dig-aquadocs-1834-375352021-07-10T02:06:02Z Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum Aminur Rahman, M. Yusoff, F.M. Arshad, A. Aquaculture Biology Fisheries Sea urchin Diadema setosum Embryo Larva Juvenile Development Pulau Pangkor aquaculture Pacific Ocean Red Sea Persian Gulf Africa Japan Australia Malaysia Diadema setosum (Leske, 1778), is one of the common echinoids widely distributed in the Indo-West Pacific Ocean, where it occurs from the Red Sea, Persian Gulf and the east coast of Africa to Japan, Australia and Malaysia. To investigate the developmental basis of morphological changes in embryos and larvae, we documented the ontogeny of D. setosum in a controlled laboratory condition at the Institute of Bioscience, Universiti Putra Malaysia, during July–September, 2012. Matured gametes were obtained from the adult individuals and the eggs fertilized at limited sperm concentration (10^-5 dilution). The obtained embryos were then reared at 24-25°C. First cleavage (2-cell), 4-cell, 8-cell, 16-cell, 32-cell and multi-cell (Morulla) stages were attained at 01.20, 02.14, 02.44, 03.09, 03.32 and 03.54 h after fertilization, respectively. Blastulae with a mean length of 111.47±1.88 µm (mean±SD) hatched 08.45 h after sperm entry. Gastrula formed 16.36 h post-fertilization and the archenteron extended constantly, while the ectodermal red-pigmented cells migrated synchronously to the apical plate. The pluteus larva started to feed unicellular algae (Chaetoceros calcitrans) in 2 d, grew continuously, and finally attained metamorphic competence within 35 d after fertilization. Induction of metamorphosis took approximately 1 h 30 min from attachment on the substratum to the complete resorption of larval tissues and the development of complete juvenile structure with adult spines, extended tubefeet and well-developed pedicellaria, the whole event usually took place within 1 d post-settlement. The newly formed juvenile (473.16 ± 6.96 µm, n=30) with a complete adult structure then grew on coralline algae to 3-month old juvenile, which represents the "sea urchin seed" for stocking in grow-out culture. This study represents the first successful investigation on embryonic, larval and early juvenile development of D. setosum. The findings would greatly be helpful towards the development of breeding and seed production techniques for aquaculture of sea urchins. 2021-06-24T18:06:19Z 2021-06-24T18:06:19Z 2015 article TRUE 1562-2916 http://hdl.handle.net/1834/37535 en http://www.ifro.ir application/pdf application/pdf 409-424 http://aquaticcommons.org/id/eprint/22785 18721 2018-05-22 18:06:23 22785 Iranian Fisheries Science Research Institute |
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Aquaculture Biology Fisheries Sea urchin Diadema setosum Embryo Larva Juvenile Development Pulau Pangkor aquaculture Pacific Ocean Red Sea Persian Gulf Africa Japan Australia Malaysia Aquaculture Biology Fisheries Sea urchin Diadema setosum Embryo Larva Juvenile Development Pulau Pangkor aquaculture Pacific Ocean Red Sea Persian Gulf Africa Japan Australia Malaysia |
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Aquaculture Biology Fisheries Sea urchin Diadema setosum Embryo Larva Juvenile Development Pulau Pangkor aquaculture Pacific Ocean Red Sea Persian Gulf Africa Japan Australia Malaysia Aquaculture Biology Fisheries Sea urchin Diadema setosum Embryo Larva Juvenile Development Pulau Pangkor aquaculture Pacific Ocean Red Sea Persian Gulf Africa Japan Australia Malaysia Aminur Rahman, M. Yusoff, F.M. Arshad, A. Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum |
description |
Diadema setosum (Leske, 1778), is one of the common echinoids widely distributed in the Indo-West Pacific Ocean, where it occurs from the Red Sea, Persian Gulf and the east coast of Africa to Japan, Australia and Malaysia. To investigate the developmental basis of morphological changes in embryos and larvae, we documented the ontogeny of D. setosum in a controlled laboratory condition at the Institute of Bioscience, Universiti Putra Malaysia, during July–September, 2012. Matured gametes were obtained from the adult individuals and the eggs fertilized at limited sperm concentration (10^-5 dilution). The obtained embryos were then reared at 24-25°C. First cleavage (2-cell), 4-cell, 8-cell, 16-cell, 32-cell and multi-cell (Morulla) stages were attained at 01.20, 02.14, 02.44, 03.09, 03.32 and 03.54 h after fertilization, respectively. Blastulae with a mean length of 111.47±1.88 µm (mean±SD) hatched 08.45 h after sperm entry. Gastrula formed 16.36 h post-fertilization and the archenteron extended constantly, while the ectodermal red-pigmented cells migrated synchronously to the apical plate. The pluteus larva started to feed unicellular algae (Chaetoceros calcitrans) in 2 d, grew continuously, and finally attained metamorphic competence within 35 d after fertilization. Induction of metamorphosis took approximately 1 h 30 min from attachment on the substratum to the complete resorption of larval tissues and the development of complete juvenile structure with adult spines, extended tubefeet and well-developed pedicellaria, the whole event usually took place within 1 d post-settlement. The newly formed juvenile (473.16 ± 6.96 µm, n=30) with a complete adult structure then grew on coralline algae to 3-month old juvenile, which represents the "sea urchin seed" for stocking in grow-out culture. This study represents the first successful investigation on embryonic, larval and early juvenile development of D. setosum. The findings would greatly be helpful towards the development of breeding and seed production techniques for aquaculture of sea urchins. |
format |
article |
topic_facet |
Aquaculture Biology Fisheries Sea urchin Diadema setosum Embryo Larva Juvenile Development Pulau Pangkor aquaculture Pacific Ocean Red Sea Persian Gulf Africa Japan Australia Malaysia |
author |
Aminur Rahman, M. Yusoff, F.M. Arshad, A. |
author_facet |
Aminur Rahman, M. Yusoff, F.M. Arshad, A. |
author_sort |
Aminur Rahman, M. |
title |
Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum |
title_short |
Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum |
title_full |
Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum |
title_fullStr |
Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum |
title_full_unstemmed |
Embryonic, larval and juvenile development of tropical sea urchin, Diadema setosum |
title_sort |
embryonic, larval and juvenile development of tropical sea urchin, diadema setosum |
publishDate |
2015 |
url |
http://hdl.handle.net/1834/37535 |
work_keys_str_mv |
AT aminurrahmanm embryoniclarvalandjuveniledevelopmentoftropicalseaurchindiademasetosum AT yusofffm embryoniclarvalandjuveniledevelopmentoftropicalseaurchindiademasetosum AT arshada embryoniclarvalandjuveniledevelopmentoftropicalseaurchindiademasetosum |
_version_ |
1756079637824274432 |