The enzymatic activity of various organs and tissues of Far-Eastern trepang as a system of indicators of maturation and quality of products
The activity, substrate-inhibitory specificity, temperature and pH dependence, seasonal changes in proteolytic and nucleolytic enzyme systems of the digestive tract, muscle and reproductive trepangs tissues have been considered. The different-rule variability of enzyme activity at different stages of development has been shown. At the stage of growth proteolytic enzymes of the intestinal tract have the greatest activity, which is significantly reduced during spawning. In muscle tissue, there are opposite processes associated with changes in the strength. Separation of proteases of the trepang digestive tract by ion exchange chromatography has been carried out. Two peaks of proteins showing trypsin activity corresponding to the isoforms of this enzyme possessing a negative charge have been detected. The molecular weight of the corresponding peaks determined by the electrophoresis method is 26 and 28 kDa. The data obtained are similar to those established earlier for other types of holothurians. Determination of the activity of proteases of trepang muscle tissue in different seasons has shown a low level of proteolytic activity during the growth period. The activity values in acid and neutral proteases are close to each other. The lowest activity has been detected in alkaline proteases. During the spawning period, the activity of acid proteases increases significantly in the muscle tissue, proteolysis remains at the same level in the neutral and alkaline zone. Reproductive tissue processes of growth and formation of the gonad is accompanied by high activity of the acidic deoxyribonucleases. At the stage of spawning it is significantly reduced, in the post-spawning period it is completely absent. Interactions of enzymes with substrates and inhibitors allow preventing the presence of cathepsins, metalloproteases and serine proteases in the trepang intestinal. The reduction of strength of the muscle tissue may be due to the action of metalloproteases affecting on the structure of the collagen matrix. The results are recommended for the development of rational methods of trepang processing by regulating the activity of enzymes.
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Format: | Journal Contribution biblioteca |
Language: | Russian |
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2018
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Subjects: | Голотурии, Протеазы, Нуклеазы, Стадии развития, Сезонные изменения, Holoturia, Proteases, Nucleases, Seasonal changes, Developmental stages, Muscles, Tissues, Animal organs, Digestive tract, Enzymes, Spawning seasons, |
Online Access: | http://hdl.handle.net/1834/16168 |
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Голотурии Протеазы Нуклеазы Стадии развития Сезонные изменения Holoturia Proteases Nucleases Seasonal changes Developmental stages Muscles Tissues Animal organs Digestive tract Enzymes Spawning seasons Голотурии Протеазы Нуклеазы Стадии развития Сезонные изменения Holoturia Proteases Nucleases Seasonal changes Developmental stages Muscles Tissues Animal organs Digestive tract Enzymes Spawning seasons |
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Голотурии Протеазы Нуклеазы Стадии развития Сезонные изменения Holoturia Proteases Nucleases Seasonal changes Developmental stages Muscles Tissues Animal organs Digestive tract Enzymes Spawning seasons Голотурии Протеазы Нуклеазы Стадии развития Сезонные изменения Holoturia Proteases Nucleases Seasonal changes Developmental stages Muscles Tissues Animal organs Digestive tract Enzymes Spawning seasons Pivnenko, T. N. Pozdnyakova, Yu. M. Kovalev, N. N. Mikheev, E. V. Esipenko, R. V. The enzymatic activity of various organs and tissues of Far-Eastern trepang as a system of indicators of maturation and quality of products |
description |
The activity, substrate-inhibitory specificity, temperature and pH dependence, seasonal changes in proteolytic and nucleolytic enzyme systems of the digestive tract, muscle and reproductive trepangs tissues have been considered. The different-rule variability of enzyme activity at different stages of development has been shown. At the stage of growth proteolytic enzymes of the intestinal tract have the greatest activity, which is significantly reduced during spawning. In muscle tissue, there are opposite processes associated with changes in the strength. Separation of proteases of the trepang digestive tract by ion exchange chromatography has been carried out. Two peaks of proteins showing trypsin activity corresponding to the isoforms of this enzyme possessing a negative charge have been detected. The molecular weight of the corresponding peaks determined by the electrophoresis method is 26 and 28 kDa. The data obtained are similar to those established earlier for other types of holothurians. Determination of the activity of proteases of trepang muscle tissue in different seasons has shown a low level of proteolytic activity during the growth period. The activity values in acid and neutral proteases are close to each other. The lowest activity has been detected in alkaline proteases. During the spawning period, the activity of acid proteases increases significantly in the muscle tissue, proteolysis remains at the same level in the neutral and alkaline zone. Reproductive tissue processes of growth and formation of the gonad is accompanied by high activity of the acidic deoxyribonucleases. At the stage of spawning it is significantly reduced, in the post-spawning period it is completely absent. Interactions of enzymes with substrates and inhibitors allow preventing the presence of cathepsins, metalloproteases and serine proteases in the trepang intestinal. The reduction of strength of the muscle tissue may be due to the action of metalloproteases affecting on the structure of the collagen matrix. The results are recommended for the development of rational methods of trepang processing by regulating the activity of enzymes. |
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Journal Contribution |
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Голотурии Протеазы Нуклеазы Стадии развития Сезонные изменения Holoturia Proteases Nucleases Seasonal changes Developmental stages Muscles Tissues Animal organs Digestive tract Enzymes Spawning seasons |
author |
Pivnenko, T. N. Pozdnyakova, Yu. M. Kovalev, N. N. Mikheev, E. V. Esipenko, R. V. |
author_facet |
Pivnenko, T. N. Pozdnyakova, Yu. M. Kovalev, N. N. Mikheev, E. V. Esipenko, R. V. |
author_sort |
Pivnenko, T. N. |
title |
The enzymatic activity of various organs and tissues of Far-Eastern trepang as a system of indicators of maturation and quality of products |
title_short |
The enzymatic activity of various organs and tissues of Far-Eastern trepang as a system of indicators of maturation and quality of products |
title_full |
The enzymatic activity of various organs and tissues of Far-Eastern trepang as a system of indicators of maturation and quality of products |
title_fullStr |
The enzymatic activity of various organs and tissues of Far-Eastern trepang as a system of indicators of maturation and quality of products |
title_full_unstemmed |
The enzymatic activity of various organs and tissues of Far-Eastern trepang as a system of indicators of maturation and quality of products |
title_sort |
enzymatic activity of various organs and tissues of far-eastern trepang as a system of indicators of maturation and quality of products |
publishDate |
2018 |
url |
http://hdl.handle.net/1834/16168 |
work_keys_str_mv |
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dig-aquadocs-1834-161682021-05-19T06:59:11Z The enzymatic activity of various organs and tissues of Far-Eastern trepang as a system of indicators of maturation and quality of products Ферментативная активность различных органов и тканей дальневосточного трепанга как система индикаторов созревания и качества продукции Pivnenko, T. N. Pozdnyakova, Yu. M. Kovalev, N. N. Mikheev, E. V. Esipenko, R. V. Голотурии Протеазы Нуклеазы Стадии развития Сезонные изменения Holoturia Proteases Nucleases Seasonal changes Developmental stages Muscles Tissues Animal organs Digestive tract Enzymes Spawning seasons The activity, substrate-inhibitory specificity, temperature and pH dependence, seasonal changes in proteolytic and nucleolytic enzyme systems of the digestive tract, muscle and reproductive trepangs tissues have been considered. The different-rule variability of enzyme activity at different stages of development has been shown. At the stage of growth proteolytic enzymes of the intestinal tract have the greatest activity, which is significantly reduced during spawning. In muscle tissue, there are opposite processes associated with changes in the strength. Separation of proteases of the trepang digestive tract by ion exchange chromatography has been carried out. Two peaks of proteins showing trypsin activity corresponding to the isoforms of this enzyme possessing a negative charge have been detected. The molecular weight of the corresponding peaks determined by the electrophoresis method is 26 and 28 kDa. The data obtained are similar to those established earlier for other types of holothurians. Determination of the activity of proteases of trepang muscle tissue in different seasons has shown a low level of proteolytic activity during the growth period. The activity values in acid and neutral proteases are close to each other. The lowest activity has been detected in alkaline proteases. During the spawning period, the activity of acid proteases increases significantly in the muscle tissue, proteolysis remains at the same level in the neutral and alkaline zone. Reproductive tissue processes of growth and formation of the gonad is accompanied by high activity of the acidic deoxyribonucleases. At the stage of spawning it is significantly reduced, in the post-spawning period it is completely absent. Interactions of enzymes with substrates and inhibitors allow preventing the presence of cathepsins, metalloproteases and serine proteases in the trepang intestinal. The reduction of strength of the muscle tissue may be due to the action of metalloproteases affecting on the structure of the collagen matrix. The results are recommended for the development of rational methods of trepang processing by regulating the activity of enzymes. Рассмотрены активность, субстратно-ингибиторная специфичность, температурная и рН зависимости, сезонные изменения протеолитических и нуклеолитических ферментных систем пищеварительного тракта, мышечной и репродуктивной тканей трепанга. Показана разнонаправленная изменчивость активности ферментов на разных стадиях развития. На стадии роста протеолитические ферменты кишечного тракта имеют наибольшую активность, которая значительно снижается в период нереста. В мышечной ткани наблюдаются противоположные процессы, связанные с изменением прочности. Проведено разделение протеаз пищеварительного тракта трепанга методом ионообменной хроматографии. Обнаружено два белковых пика, проявляющих активность трипсина, соответствующих обладающим отрицательным зарядом изоформам этого фермента. Молекулярная масса соответствующих пиков, определенная методом электрофореза, составила 26 и 28 кДа. Полученные данные аналогичны установленным ранее для других видов голотурий. Определение активности протеаз мышечной ткани трепанга в разные сезоны показало невысокий уровень протеолитической активности в период роста. Величины активности кислых и нейтральных протеаз близки между собой. Наименьшая активность выявлена у щелочных протеаз. В период нереста в мышечной ткани значительно повышается активность кислых протеаз, в нейтральной и щелочной зоне протеолиз остается на прежнем уровне. Снижение прочности мышечной ткани трепанга может быть связано с действием металлопротеаз, влияющих на структуру коллагеновой матрицы. Для репродуктивной ткани процессы роста и формирования гонад сопровождаются высокой активностью кислых дезоксирибонуклеаз. На стадии нереста она значительно снижается, в посленерестовом периоде отсутствует полностью. Взаимодействия ферментов с субстратами и ингибиторами позволяют предполагать присутствие катепсинов, металлопротеаз и сериновых протеаз в кишечном тракте трепанга. Полученные результаты рекомендованы для разработки рациональных способов переработки трепанга путем регуляции активности ферментов. Published 2020-02-10T12:01:16Z 2020-02-10T12:01:16Z 2018 Journal Contribution Refereed 1560-9278 10.21443/1560-9278-2018-21-3-402-411 http://hdl.handle.net/1834/16168 ru http://vestnik.mstu.edu.ru/show-eng.shtml?art=1963 pp.402-411 |