Method for obtaining high-resolution proteomic analysis from pericarps of guarana.

Guarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract preparation, which needs to offer an extract that is free of contaminants. This study aimed to evaluate different extraction methods to obtain high-quantity and high-quality extracts that are compatible with analysis by 2-dimensional electrophoresis and tandem mass spectrometry protein identification. Three different methods were tested: trichloroacetic acid (TCA)/acetone, sodium dodecyl sulfate (SDS)/phenol, and polyvinylpolypyrrolidone (PVPP)/SDS/phenol.

Bibliographic Details

Main Authors:	SOUZA, A. L., ANGELO, P. C. da S., NOGUEIRA, P. P. O., GONÇALVES, J. F. C., FRANCO, A. M., ASTOLFI-FILHO, S., LÓPEZ-LOZANO, J. L., ANDRADE, E. V.
Other Authors:	A. L. Souza, UFAM; PAULA CRISTINA DA SILVA ANGELO, CPAA; P. P. O. Nogueira, FIOCRUZ; J. F. C. Gonçalves, INPA; A. M. Franco, INPA; S. Astolfi-Filho, UFAM; J. L. López-Lozano, Fundação de Medicina Tropical do Amazonas; E. V. Andrade, UFAM.
Format:	Separatas biblioteca
Language:	English eng
Published:	2014-10-08
Subjects:	Plant proteomics, Two-dimensional electrophoresis., Guaraná, Paullinia Cupana., polyphenols.,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/996859 http://dx.doi.org/10.4238/2014.September.29.14
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