Backcross assisted by microsatellite markers in common bean.
The objectives of the present study were to monitor the effect of backcrossing through microsatellite markers and to compare different marker assisted selection strategies. Four populations were developed using donor parents resistant to the bean golden mosaic virusand, for all crosses, only individuals resistant to the bean golden mosaic virus were backcrossed. For crosses ARC100-4 x DOR303 and ARC100-4 x PHAS8328, assisted selection was carried out in the F2 and F2BC1generations, while in the remaining crosses selection was performed only in the F2BC1 generation. For the microsatellite analysis, in each generation, 20 markers were genotyped. The molecular data were analyzed using the NTSys program and the proportion of the recurrent genome introgressed was estimated, based on genotypical configuration of the segregant populations compared to the recurrent parents. The results indicate a higher efficiency in recovering the genotype of the elite genitor through the strategy of backcross assisted selection in the successive generations, and demonstrate a practical and useful application of molecular marker technology associated with bean breeding, to reduce the number of backcrosses and the time to recover the genome of the recurrent genitors.
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Format: | Artigo de periódico biblioteca |
Language: | Ingles English |
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2008-10-09
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Subjects: | Molecular markers, Genetic breeding, Feijão, Melhoramento Genético Vegetal, Phaseolus Vulgaris, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/217398 |
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dig-alice-doc-2173982021-07-21T18:01:26Z Backcross assisted by microsatellite markers in common bean. OLIVEIRA, L. K. MELO, L. C. BRONDANI, C. DEL PELOSO, M. J. BRONDANI, R. P. V. L. K. OLIVEIRA, Universidade Católica de Goiás; LEONARDO CUNHA MELO, CNPAF; CLAUDIO BRONDANI, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. Molecular markers Genetic breeding Feijão Melhoramento Genético Vegetal Phaseolus Vulgaris The objectives of the present study were to monitor the effect of backcrossing through microsatellite markers and to compare different marker assisted selection strategies. Four populations were developed using donor parents resistant to the bean golden mosaic virusand, for all crosses, only individuals resistant to the bean golden mosaic virus were backcrossed. For crosses ARC100-4 x DOR303 and ARC100-4 x PHAS8328, assisted selection was carried out in the F2 and F2BC1generations, while in the remaining crosses selection was performed only in the F2BC1 generation. For the microsatellite analysis, in each generation, 20 markers were genotyped. The molecular data were analyzed using the NTSys program and the proportion of the recurrent genome introgressed was estimated, based on genotypical configuration of the segregant populations compared to the recurrent parents. The results indicate a higher efficiency in recovering the genotype of the elite genitor through the strategy of backcross assisted selection in the successive generations, and demonstrate a practical and useful application of molecular marker technology associated with bean breeding, to reduce the number of backcrosses and the time to recover the genome of the recurrent genitors. 2021-07-21T18:01:20Z 2021-07-21T18:01:20Z 2008-10-09 2008 Artigo de periódico Genetics and Molecular Research, v. 7, n. 4, p. 1000-1010, 2008. http://www.alice.cnptia.embrapa.br/alice/handle/doc/217398 Ingles en openAccess |
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Molecular markers Genetic breeding Feijão Melhoramento Genético Vegetal Phaseolus Vulgaris Molecular markers Genetic breeding Feijão Melhoramento Genético Vegetal Phaseolus Vulgaris |
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Molecular markers Genetic breeding Feijão Melhoramento Genético Vegetal Phaseolus Vulgaris Molecular markers Genetic breeding Feijão Melhoramento Genético Vegetal Phaseolus Vulgaris OLIVEIRA, L. K. MELO, L. C. BRONDANI, C. DEL PELOSO, M. J. BRONDANI, R. P. V. Backcross assisted by microsatellite markers in common bean. |
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The objectives of the present study were to monitor the effect of backcrossing through microsatellite markers and to compare different marker assisted selection strategies. Four populations were developed using donor parents resistant to the bean golden mosaic virusand, for all crosses, only individuals resistant to the bean golden mosaic virus were backcrossed. For crosses ARC100-4 x DOR303 and ARC100-4 x PHAS8328, assisted selection was carried out in the F2 and F2BC1generations, while in the remaining crosses selection was performed only in the F2BC1 generation. For the microsatellite analysis, in each generation, 20 markers were genotyped. The molecular data were analyzed using the NTSys program and the proportion of the recurrent genome introgressed was estimated, based on genotypical configuration of the segregant populations compared to the recurrent parents. The results indicate a higher efficiency in recovering the genotype of the elite genitor through the strategy of backcross assisted selection in the successive generations, and demonstrate a practical and useful application of molecular marker technology associated with bean breeding, to reduce the number of backcrosses and the time to recover the genome of the recurrent genitors. |
author2 |
L. K. OLIVEIRA, Universidade Católica de Goiás; LEONARDO CUNHA MELO, CNPAF; CLAUDIO BRONDANI, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
author_facet |
L. K. OLIVEIRA, Universidade Católica de Goiás; LEONARDO CUNHA MELO, CNPAF; CLAUDIO BRONDANI, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. OLIVEIRA, L. K. MELO, L. C. BRONDANI, C. DEL PELOSO, M. J. BRONDANI, R. P. V. |
format |
Artigo de periódico |
topic_facet |
Molecular markers Genetic breeding Feijão Melhoramento Genético Vegetal Phaseolus Vulgaris |
author |
OLIVEIRA, L. K. MELO, L. C. BRONDANI, C. DEL PELOSO, M. J. BRONDANI, R. P. V. |
author_sort |
OLIVEIRA, L. K. |
title |
Backcross assisted by microsatellite markers in common bean. |
title_short |
Backcross assisted by microsatellite markers in common bean. |
title_full |
Backcross assisted by microsatellite markers in common bean. |
title_fullStr |
Backcross assisted by microsatellite markers in common bean. |
title_full_unstemmed |
Backcross assisted by microsatellite markers in common bean. |
title_sort |
backcross assisted by microsatellite markers in common bean. |
publishDate |
2008-10-09 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/217398 |
work_keys_str_mv |
AT oliveiralk backcrossassistedbymicrosatellitemarkersincommonbean AT melolc backcrossassistedbymicrosatellitemarkersincommonbean AT brondanic backcrossassistedbymicrosatellitemarkersincommonbean AT delpelosomj backcrossassistedbymicrosatellitemarkersincommonbean AT brondanirpv backcrossassistedbymicrosatellitemarkersincommonbean |
_version_ |
1756027608673288192 |