Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization.
The use of fluorescent-labelled simple sequence repeat (SSR) markers allows the simultaneous analysis of diverse loci in multiplex series, providing an enhanced precision of the determination of the allele size when a large number of genotypes is used. Twenty-five fluorescent-labelled SSR markers were used to genotype 242 accessions of the Brazilian Rice Core Collection. Based on the Polymorphism Information Content (PIC) values of each marker, groups with 5, 10, 15 and 20 markers were formed. The average PIC values varied from 0.67 to 0.89 and the number of alleles sampled per group, from 70 to 377. In the case of rice, which has a narrow genetic base, the conclusion was drawn that it is of fundamental importance that the genotypes must be evaluated by the highest possible number of regularly spaced SSR markers in the genome, and that these, preferentially, should offer a high information content.
Main Authors: | , , , |
---|---|
Other Authors: | |
Format: | Artigo de periódico biblioteca |
Language: | English eng |
Published: |
2005-08-19
|
Subjects: | Diversidade genética, Recursos genéticos, Genetic diversity., Arroz, Oryza Sativa., genetic resources, rice., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/213598 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-alice-doc-213598 |
---|---|
record_format |
koha |
spelling |
dig-alice-doc-2135982017-08-15T22:34:07Z Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. BORBA, T. C. de O. BRONDANI, R. V. RANGEL, P. H. N. BRONDANI, C. TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; ROSANA PEREIRA VIANELLO BRONDANI, CNPAF; PAULO HIDEO NAKANO RANGEL, CNPAF; CLAUDIO BRONDANI, CNPAF. Diversidade genética Recursos genéticos Genetic diversity. Arroz Oryza Sativa. genetic resources rice. The use of fluorescent-labelled simple sequence repeat (SSR) markers allows the simultaneous analysis of diverse loci in multiplex series, providing an enhanced precision of the determination of the allele size when a large number of genotypes is used. Twenty-five fluorescent-labelled SSR markers were used to genotype 242 accessions of the Brazilian Rice Core Collection. Based on the Polymorphism Information Content (PIC) values of each marker, groups with 5, 10, 15 and 20 markers were formed. The average PIC values varied from 0.67 to 0.89 and the number of alleles sampled per group, from 70 to 377. In the case of rice, which has a narrow genetic base, the conclusion was drawn that it is of fundamental importance that the genotypes must be evaluated by the highest possible number of regularly spaced SSR markers in the genome, and that these, preferentially, should offer a high information content. 2011-04-09T14:33:24Z 2011-04-09T14:33:24Z 2005-08-19 2005 2011-04-10T11:11:11Z Artigo de periódico Crop Breeding and Applied Biotechnology, v. 5, n. 2, p. 157-165, June 2005. http://www.alice.cnptia.embrapa.br/alice/handle/doc/213598 en eng openAccess |
institution |
EMBRAPA |
collection |
DSpace |
country |
Brasil |
countrycode |
BR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-alice |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Sistema de bibliotecas de EMBRAPA |
language |
English eng |
topic |
Diversidade genética Recursos genéticos Genetic diversity. Arroz Oryza Sativa. genetic resources rice. Diversidade genética Recursos genéticos Genetic diversity. Arroz Oryza Sativa. genetic resources rice. |
spellingShingle |
Diversidade genética Recursos genéticos Genetic diversity. Arroz Oryza Sativa. genetic resources rice. Diversidade genética Recursos genéticos Genetic diversity. Arroz Oryza Sativa. genetic resources rice. BORBA, T. C. de O. BRONDANI, R. V. RANGEL, P. H. N. BRONDANI, C. Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. |
description |
The use of fluorescent-labelled simple sequence repeat (SSR) markers allows the simultaneous analysis of diverse loci in multiplex series, providing an enhanced precision of the determination of the allele size when a large number of genotypes is used. Twenty-five fluorescent-labelled SSR markers were used to genotype 242 accessions of the Brazilian Rice Core Collection. Based on the Polymorphism Information Content (PIC) values of each marker, groups with 5, 10, 15 and 20 markers were formed. The average PIC values varied from 0.67 to 0.89 and the number of alleles sampled per group, from 70 to 377. In the case of rice, which has a narrow genetic base, the conclusion was drawn that it is of fundamental importance that the genotypes must be evaluated by the highest possible number of regularly spaced SSR markers in the genome, and that these, preferentially, should offer a high information content. |
author2 |
TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; ROSANA PEREIRA VIANELLO BRONDANI, CNPAF; PAULO HIDEO NAKANO RANGEL, CNPAF; CLAUDIO BRONDANI, CNPAF. |
author_facet |
TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; ROSANA PEREIRA VIANELLO BRONDANI, CNPAF; PAULO HIDEO NAKANO RANGEL, CNPAF; CLAUDIO BRONDANI, CNPAF. BORBA, T. C. de O. BRONDANI, R. V. RANGEL, P. H. N. BRONDANI, C. |
format |
Artigo de periódico |
topic_facet |
Diversidade genética Recursos genéticos Genetic diversity. Arroz Oryza Sativa. genetic resources rice. |
author |
BORBA, T. C. de O. BRONDANI, R. V. RANGEL, P. H. N. BRONDANI, C. |
author_sort |
BORBA, T. C. de O. |
title |
Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. |
title_short |
Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. |
title_full |
Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. |
title_fullStr |
Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. |
title_full_unstemmed |
Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. |
title_sort |
evaluation of the number and information content of fluorescent-labeled ssr markers for rice germplasm characterization. |
publishDate |
2005-08-19 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/213598 |
work_keys_str_mv |
AT borbatcdeo evaluationofthenumberandinformationcontentoffluorescentlabeledssrmarkersforricegermplasmcharacterization AT brondanirv evaluationofthenumberandinformationcontentoffluorescentlabeledssrmarkersforricegermplasmcharacterization AT rangelphn evaluationofthenumberandinformationcontentoffluorescentlabeledssrmarkersforricegermplasmcharacterization AT brondanic evaluationofthenumberandinformationcontentoffluorescentlabeledssrmarkersforricegermplasmcharacterization |
_version_ |
1756014179342352384 |