Presence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers.
Two Acinetobacter spp. strains, denominated D-12 and D-16 (extracted from Diuron TM treated sugarcane rhizosphere) were grown in different substrates in order to determine whether catechol 1,2-dioxygenase (pyrocatechase) and catechol 2,3-dioxygenase (metapyrocatechase) are constitutive or induced enzymes. Extracellular enzymes were also measured. Although biomass was measured, the conditions required for best production of microorganisms were not established. Five different inducers were added to culture media: glucose, sodium benzoate, Diuron TM, dichloroaniline and Diuron TM plus glucose. Both strains were harvested by centrifugation and disrupted by sonication for intracellular enzyme extraction. Extracellular enzyme assays were carried out with filtered supernatant. Both strains showed significant catechol 1,2-dioxygenase activity when sodium benzoate was added, but not with other inducers. Significant manganese (MnP) and lignin (LiP) peroxidase activity was observed in all supernatants, except for D-16 in which LiP was not detected with sodium benzoate. Data obtained showed evidence of the inductive nature of catechol 1,2-dioxygenase.
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1998-05-26
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Subjects: | Extracellular enzymes, Acinetobacter spp, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/12899 |
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dig-alice-doc-128992019-05-08T00:35:40Z Presence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers. SCRAMIN, S. ROQUE, M. R. A. DENTZIEN, A. F. M. ROSSO, C. R. S. de MELO, I. S. de SHIRLEI SCRAMIN, EMBRAPA-CNPMA; ANAMARIA FERREIRA MAYER DENTZIEN, CNPMA; CESAR ROBERTO SILVA DE ROSSO, CNPMA; ITAMAR SOARES DE MELO, CNPMA. Extracellular enzymes Acinetobacter spp Two Acinetobacter spp. strains, denominated D-12 and D-16 (extracted from Diuron TM treated sugarcane rhizosphere) were grown in different substrates in order to determine whether catechol 1,2-dioxygenase (pyrocatechase) and catechol 2,3-dioxygenase (metapyrocatechase) are constitutive or induced enzymes. Extracellular enzymes were also measured. Although biomass was measured, the conditions required for best production of microorganisms were not established. Five different inducers were added to culture media: glucose, sodium benzoate, Diuron TM, dichloroaniline and Diuron TM plus glucose. Both strains were harvested by centrifugation and disrupted by sonication for intracellular enzyme extraction. Extracellular enzyme assays were carried out with filtered supernatant. Both strains showed significant catechol 1,2-dioxygenase activity when sodium benzoate was added, but not with other inducers. Significant manganese (MnP) and lignin (LiP) peroxidase activity was observed in all supernatants, except for D-16 in which LiP was not detected with sodium benzoate. Data obtained showed evidence of the inductive nature of catechol 1,2-dioxygenase. 2019-05-08T00:35:29Z 2019-05-08T00:35:29Z 1998-05-26 1998 2019-05-08T00:35:29Z Separatas In: LATIN AMERICAN BIODEGRADATION & BIODETERIORATION SYMPOSIUM, 3., 1998, Florianopolis. Resumos. Florianopolis: UFSC, 1998. http://www.alice.cnptia.embrapa.br/alice/handle/doc/12899 en eng openAccess p.16 |
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Extracellular enzymes Acinetobacter spp Extracellular enzymes Acinetobacter spp |
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Extracellular enzymes Acinetobacter spp Extracellular enzymes Acinetobacter spp SCRAMIN, S. ROQUE, M. R. A. DENTZIEN, A. F. M. ROSSO, C. R. S. de MELO, I. S. de Presence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers. |
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Two Acinetobacter spp. strains, denominated D-12 and D-16 (extracted from Diuron TM treated sugarcane rhizosphere) were grown in different substrates in order to determine whether catechol 1,2-dioxygenase (pyrocatechase) and catechol 2,3-dioxygenase (metapyrocatechase) are constitutive or induced enzymes. Extracellular enzymes were also measured. Although biomass was measured, the conditions required for best production of microorganisms were not established. Five different inducers were added to culture media: glucose, sodium benzoate, Diuron TM, dichloroaniline and Diuron TM plus glucose. Both strains were harvested by centrifugation and disrupted by sonication for intracellular enzyme extraction. Extracellular enzyme assays were carried out with filtered supernatant. Both strains showed significant catechol 1,2-dioxygenase activity when sodium benzoate was added, but not with other inducers. Significant manganese (MnP) and lignin (LiP) peroxidase activity was observed in all supernatants, except for D-16 in which LiP was not detected with sodium benzoate. Data obtained showed evidence of the inductive nature of catechol 1,2-dioxygenase. |
author2 |
SHIRLEI SCRAMIN, EMBRAPA-CNPMA; ANAMARIA FERREIRA MAYER DENTZIEN, CNPMA; CESAR ROBERTO SILVA DE ROSSO, CNPMA; ITAMAR SOARES DE MELO, CNPMA. |
author_facet |
SHIRLEI SCRAMIN, EMBRAPA-CNPMA; ANAMARIA FERREIRA MAYER DENTZIEN, CNPMA; CESAR ROBERTO SILVA DE ROSSO, CNPMA; ITAMAR SOARES DE MELO, CNPMA. SCRAMIN, S. ROQUE, M. R. A. DENTZIEN, A. F. M. ROSSO, C. R. S. de MELO, I. S. de |
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topic_facet |
Extracellular enzymes Acinetobacter spp |
author |
SCRAMIN, S. ROQUE, M. R. A. DENTZIEN, A. F. M. ROSSO, C. R. S. de MELO, I. S. de |
author_sort |
SCRAMIN, S. |
title |
Presence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers. |
title_short |
Presence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers. |
title_full |
Presence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers. |
title_fullStr |
Presence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers. |
title_full_unstemmed |
Presence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers. |
title_sort |
presence of intra and extracellular enzymes in strains of acinetobacter spp, produced in different inducers. |
publishDate |
1998-05-26 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/12899 |
work_keys_str_mv |
AT scramins presenceofintraandextracellularenzymesinstrainsofacinetobactersppproducedindifferentinducers AT roquemra presenceofintraandextracellularenzymesinstrainsofacinetobactersppproducedindifferentinducers AT dentzienafm presenceofintraandextracellularenzymesinstrainsofacinetobactersppproducedindifferentinducers AT rossocrsde presenceofintraandextracellularenzymesinstrainsofacinetobactersppproducedindifferentinducers AT meloisde presenceofintraandextracellularenzymesinstrainsofacinetobactersppproducedindifferentinducers |
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