Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4- Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA. Hancornia speciosa Gomes pertenece a la familia Apocynaceae, distribuida en diferentes regiones de Brasil. El objetivo de este trabajo fue evaluar la callogénesis y la histodiferenciación en cinco accesiones de mangaba bajo diferentes condiciones de cultivo in vitro. Se evaluaron cinco accesiones del Banco de Germoplasma Activo de Mangaba (Embrapa, Brasil). Las plántulas germinadas in vitro se utilizaron para la escisión de explantes (segmentos nodales e internodales y secciones foliares). Estos segmentos fueron inoculados en un medio de cultivo que contenía diferentes concentraciones de 6-benzilaminopurina (BA) y ácido 2,4-diclorofenoxiacético (2,4-D). Hubo un efecto significativo de las accesiones, los tratamientos y el tiempo del cultivo en la masa de los callos. No hubo inducción de callos en ausencia de reguladores. A los 60 días de cultivo in vitro, los otros tratamientos presentaron un crecimiento celular linear positivo. La mayor masa de callo se observó en la accesión BI, en presencia de 22,62 μM 2,4-D y 11,10 μM BA.
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Format: | Artigo de periódico biblioteca |
Language: | Ingles English |
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2023-12-18
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Subjects: | Mangaba, Hancornia Speciosa, Cultura de Tecido, Tecido Vegetal, Genética Vegetal, Tropical and subtropical fruits, Plant genetics, Tissue culture, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938 https://doi.org/10.14483/2256201X.19696 |
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dig-alice-doc-11599382023-12-18T20:33:31Z Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. LEDO, A. da S. MACHADO, C. de A. OLIVEIRA, A. C. A. de ARRIGONI BLANK, M. de CASTRO, E. M. de SILVA, A. V. C. da ANA DA SILVA LEDO, CPATC; CAROLINE DE ARAUJO MACHADO, UFS; ANNIE CAROLINA ARAÚJO DE OLIVEIRA, UFS; MARIA DE FÁTIMA ARRIGONI BLANK, UFS; EVARISTO MAURO DE CASTRO, UNIVERSIDADE FEDERAL DE LAVRAS; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC. Mangaba Hancornia Speciosa Cultura de Tecido Tecido Vegetal Genética Vegetal Tropical and subtropical fruits Plant genetics Tissue culture Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4- Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA. Hancornia speciosa Gomes pertenece a la familia Apocynaceae, distribuida en diferentes regiones de Brasil. El objetivo de este trabajo fue evaluar la callogénesis y la histodiferenciación en cinco accesiones de mangaba bajo diferentes condiciones de cultivo in vitro. Se evaluaron cinco accesiones del Banco de Germoplasma Activo de Mangaba (Embrapa, Brasil). Las plántulas germinadas in vitro se utilizaron para la escisión de explantes (segmentos nodales e internodales y secciones foliares). Estos segmentos fueron inoculados en un medio de cultivo que contenía diferentes concentraciones de 6-benzilaminopurina (BA) y ácido 2,4-diclorofenoxiacético (2,4-D). Hubo un efecto significativo de las accesiones, los tratamientos y el tiempo del cultivo en la masa de los callos. No hubo inducción de callos en ausencia de reguladores. A los 60 días de cultivo in vitro, los otros tratamientos presentaron un crecimiento celular linear positivo. La mayor masa de callo se observó en la accesión BI, en presencia de 22,62 μM 2,4-D y 11,10 μM BA. 2023-12-18T20:33:31Z 2023-12-18T20:33:31Z 2023-12-18 2023 Artigo de periódico Colombia Forestal, v. 26, n. 2, p. 5-14, ju./dez. 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938 https://doi.org/10.14483/2256201X.19696 Ingles en openAccess |
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Mangaba Hancornia Speciosa Cultura de Tecido Tecido Vegetal Genética Vegetal Tropical and subtropical fruits Plant genetics Tissue culture Mangaba Hancornia Speciosa Cultura de Tecido Tecido Vegetal Genética Vegetal Tropical and subtropical fruits Plant genetics Tissue culture |
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Mangaba Hancornia Speciosa Cultura de Tecido Tecido Vegetal Genética Vegetal Tropical and subtropical fruits Plant genetics Tissue culture Mangaba Hancornia Speciosa Cultura de Tecido Tecido Vegetal Genética Vegetal Tropical and subtropical fruits Plant genetics Tissue culture LEDO, A. da S. MACHADO, C. de A. OLIVEIRA, A. C. A. de ARRIGONI BLANK, M. de CASTRO, E. M. de SILVA, A. V. C. da Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. |
description |
Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4- Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA. Hancornia speciosa Gomes pertenece a la familia Apocynaceae, distribuida en diferentes regiones de Brasil. El objetivo de este trabajo fue evaluar la callogénesis y la histodiferenciación en cinco accesiones de mangaba bajo diferentes condiciones de cultivo in vitro. Se evaluaron cinco accesiones del Banco de Germoplasma Activo de Mangaba (Embrapa, Brasil). Las plántulas germinadas in vitro se utilizaron para la escisión de explantes (segmentos nodales e internodales y secciones foliares). Estos segmentos fueron inoculados en un medio de cultivo que contenía diferentes concentraciones de 6-benzilaminopurina (BA) y ácido 2,4-diclorofenoxiacético (2,4-D). Hubo un efecto significativo de las accesiones, los tratamientos y el tiempo del cultivo en la masa de los callos. No hubo inducción de callos en ausencia de reguladores. A los 60 días de cultivo in vitro, los otros tratamientos presentaron un crecimiento celular linear positivo. La mayor masa de callo se observó en la accesión BI, en presencia de 22,62 μM 2,4-D y 11,10 μM BA. |
author2 |
ANA DA SILVA LEDO, CPATC; CAROLINE DE ARAUJO MACHADO, UFS; ANNIE CAROLINA ARAÚJO DE OLIVEIRA, UFS; MARIA DE FÁTIMA ARRIGONI BLANK, UFS; EVARISTO MAURO DE CASTRO, UNIVERSIDADE FEDERAL DE LAVRAS; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC. |
author_facet |
ANA DA SILVA LEDO, CPATC; CAROLINE DE ARAUJO MACHADO, UFS; ANNIE CAROLINA ARAÚJO DE OLIVEIRA, UFS; MARIA DE FÁTIMA ARRIGONI BLANK, UFS; EVARISTO MAURO DE CASTRO, UNIVERSIDADE FEDERAL DE LAVRAS; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC. LEDO, A. da S. MACHADO, C. de A. OLIVEIRA, A. C. A. de ARRIGONI BLANK, M. de CASTRO, E. M. de SILVA, A. V. C. da |
format |
Artigo de periódico |
topic_facet |
Mangaba Hancornia Speciosa Cultura de Tecido Tecido Vegetal Genética Vegetal Tropical and subtropical fruits Plant genetics Tissue culture |
author |
LEDO, A. da S. MACHADO, C. de A. OLIVEIRA, A. C. A. de ARRIGONI BLANK, M. de CASTRO, E. M. de SILVA, A. V. C. da |
author_sort |
LEDO, A. da S. |
title |
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. |
title_short |
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. |
title_full |
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. |
title_fullStr |
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. |
title_full_unstemmed |
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. |
title_sort |
callogenesis and morphohistological characterization of hancornia speciosa gomes. |
publishDate |
2023-12-18 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938 https://doi.org/10.14483/2256201X.19696 |
work_keys_str_mv |
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