Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.

Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4- Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA. Hancornia speciosa Gomes pertenece a la familia Apocynaceae, distribuida en diferentes regiones de Brasil. El objetivo de este trabajo fue evaluar la callogénesis y la histodiferenciación en cinco accesiones de mangaba bajo diferentes condiciones de cultivo in vitro. Se evaluaron cinco accesiones del Banco de Germoplasma Activo de Mangaba (Embrapa, Brasil). Las plántulas germinadas in vitro se utilizaron para la escisión de explantes (segmentos nodales e internodales y secciones foliares). Estos segmentos fueron inoculados en un medio de cultivo que contenía diferentes concentraciones de 6-benzilaminopurina (BA) y ácido 2,4-diclorofenoxiacético (2,4-D). Hubo un efecto significativo de las accesiones, los tratamientos y el tiempo del cultivo en la masa de los callos. No hubo inducción de callos en ausencia de reguladores. A los 60 días de cultivo in vitro, los otros tratamientos presentaron un crecimiento celular linear positivo. La mayor masa de callo se observó en la accesión BI, en presencia de 22,62 μM 2,4-D y 11,10 μM BA.

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Main Authors: LEDO, A. da S., MACHADO, C. de A., OLIVEIRA, A. C. A. de, ARRIGONI BLANK, M. de, CASTRO, E. M. de, SILVA, A. V. C. da
Other Authors: ANA DA SILVA LEDO, CPATC; CAROLINE DE ARAUJO MACHADO, UFS; ANNIE CAROLINA ARAÚJO DE OLIVEIRA, UFS; MARIA DE FÁTIMA ARRIGONI BLANK, UFS; EVARISTO MAURO DE CASTRO, UNIVERSIDADE FEDERAL DE LAVRAS; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC.
Format: Artigo de periódico biblioteca
Language:Ingles
English
Published: 2023-12-18
Subjects:Mangaba, Hancornia Speciosa, Cultura de Tecido, Tecido Vegetal, Genética Vegetal, Tropical and subtropical fruits, Plant genetics, Tissue culture,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938
https://doi.org/10.14483/2256201X.19696
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spelling dig-alice-doc-11599382023-12-18T20:33:31Z Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. LEDO, A. da S. MACHADO, C. de A. OLIVEIRA, A. C. A. de ARRIGONI BLANK, M. de CASTRO, E. M. de SILVA, A. V. C. da ANA DA SILVA LEDO, CPATC; CAROLINE DE ARAUJO MACHADO, UFS; ANNIE CAROLINA ARAÚJO DE OLIVEIRA, UFS; MARIA DE FÁTIMA ARRIGONI BLANK, UFS; EVARISTO MAURO DE CASTRO, UNIVERSIDADE FEDERAL DE LAVRAS; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC. Mangaba Hancornia Speciosa Cultura de Tecido Tecido Vegetal Genética Vegetal Tropical and subtropical fruits Plant genetics Tissue culture Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4- Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA. Hancornia speciosa Gomes pertenece a la familia Apocynaceae, distribuida en diferentes regiones de Brasil. El objetivo de este trabajo fue evaluar la callogénesis y la histodiferenciación en cinco accesiones de mangaba bajo diferentes condiciones de cultivo in vitro. Se evaluaron cinco accesiones del Banco de Germoplasma Activo de Mangaba (Embrapa, Brasil). Las plántulas germinadas in vitro se utilizaron para la escisión de explantes (segmentos nodales e internodales y secciones foliares). Estos segmentos fueron inoculados en un medio de cultivo que contenía diferentes concentraciones de 6-benzilaminopurina (BA) y ácido 2,4-diclorofenoxiacético (2,4-D). Hubo un efecto significativo de las accesiones, los tratamientos y el tiempo del cultivo en la masa de los callos. No hubo inducción de callos en ausencia de reguladores. A los 60 días de cultivo in vitro, los otros tratamientos presentaron un crecimiento celular linear positivo. La mayor masa de callo se observó en la accesión BI, en presencia de 22,62 μM 2,4-D y 11,10 μM BA. 2023-12-18T20:33:31Z 2023-12-18T20:33:31Z 2023-12-18 2023 Artigo de periódico Colombia Forestal, v. 26, n. 2, p. 5-14, ju./dez. 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938 https://doi.org/10.14483/2256201X.19696 Ingles en openAccess
institution EMBRAPA
collection DSpace
country Brasil
countrycode BR
component Bibliográfico
access En linea
databasecode dig-alice
tag biblioteca
region America del Sur
libraryname Sistema de bibliotecas de EMBRAPA
language Ingles
English
topic Mangaba
Hancornia Speciosa
Cultura de Tecido
Tecido Vegetal
Genética Vegetal
Tropical and subtropical fruits
Plant genetics
Tissue culture
Mangaba
Hancornia Speciosa
Cultura de Tecido
Tecido Vegetal
Genética Vegetal
Tropical and subtropical fruits
Plant genetics
Tissue culture
spellingShingle Mangaba
Hancornia Speciosa
Cultura de Tecido
Tecido Vegetal
Genética Vegetal
Tropical and subtropical fruits
Plant genetics
Tissue culture
Mangaba
Hancornia Speciosa
Cultura de Tecido
Tecido Vegetal
Genética Vegetal
Tropical and subtropical fruits
Plant genetics
Tissue culture
LEDO, A. da S.
MACHADO, C. de A.
OLIVEIRA, A. C. A. de
ARRIGONI BLANK, M. de
CASTRO, E. M. de
SILVA, A. V. C. da
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
description Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4- Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA. Hancornia speciosa Gomes pertenece a la familia Apocynaceae, distribuida en diferentes regiones de Brasil. El objetivo de este trabajo fue evaluar la callogénesis y la histodiferenciación en cinco accesiones de mangaba bajo diferentes condiciones de cultivo in vitro. Se evaluaron cinco accesiones del Banco de Germoplasma Activo de Mangaba (Embrapa, Brasil). Las plántulas germinadas in vitro se utilizaron para la escisión de explantes (segmentos nodales e internodales y secciones foliares). Estos segmentos fueron inoculados en un medio de cultivo que contenía diferentes concentraciones de 6-benzilaminopurina (BA) y ácido 2,4-diclorofenoxiacético (2,4-D). Hubo un efecto significativo de las accesiones, los tratamientos y el tiempo del cultivo en la masa de los callos. No hubo inducción de callos en ausencia de reguladores. A los 60 días de cultivo in vitro, los otros tratamientos presentaron un crecimiento celular linear positivo. La mayor masa de callo se observó en la accesión BI, en presencia de 22,62 μM 2,4-D y 11,10 μM BA.
author2 ANA DA SILVA LEDO, CPATC; CAROLINE DE ARAUJO MACHADO, UFS; ANNIE CAROLINA ARAÚJO DE OLIVEIRA, UFS; MARIA DE FÁTIMA ARRIGONI BLANK, UFS; EVARISTO MAURO DE CASTRO, UNIVERSIDADE FEDERAL DE LAVRAS; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC.
author_facet ANA DA SILVA LEDO, CPATC; CAROLINE DE ARAUJO MACHADO, UFS; ANNIE CAROLINA ARAÚJO DE OLIVEIRA, UFS; MARIA DE FÁTIMA ARRIGONI BLANK, UFS; EVARISTO MAURO DE CASTRO, UNIVERSIDADE FEDERAL DE LAVRAS; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC.
LEDO, A. da S.
MACHADO, C. de A.
OLIVEIRA, A. C. A. de
ARRIGONI BLANK, M. de
CASTRO, E. M. de
SILVA, A. V. C. da
format Artigo de periódico
topic_facet Mangaba
Hancornia Speciosa
Cultura de Tecido
Tecido Vegetal
Genética Vegetal
Tropical and subtropical fruits
Plant genetics
Tissue culture
author LEDO, A. da S.
MACHADO, C. de A.
OLIVEIRA, A. C. A. de
ARRIGONI BLANK, M. de
CASTRO, E. M. de
SILVA, A. V. C. da
author_sort LEDO, A. da S.
title Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
title_short Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
title_full Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
title_fullStr Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
title_full_unstemmed Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
title_sort callogenesis and morphohistological characterization of hancornia speciosa gomes.
publishDate 2023-12-18
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938
https://doi.org/10.14483/2256201X.19696
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