Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant.
Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to have the highest vitamin C content of any edible fruit (13.757 mg 100 g-1 dry weight). The objective of this study was to evaluate the effects of 2,4-D and 6-BAP alone or in combination on in vitro callus induction and pro-embryogenic mass formation in caçari stem segments in different culture media as a precursor for caçari micropropagation. The experiment consisted of two culture media (MS and WPM) and combinations of four concentrations of 2,4-D (0, 1, 2 and 4 mg L-1) and BAP (0, 0.25, 0.5 and 1 mg L-1). The results showed that the highest percentage of callus formation (99%) was obtained in WPM supplemented with 4 mg L-1, 2,4-D and 1 mg L-1 BAP, and this combination resulted in 93% pro-embryogenic mass formation.
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Format: | Artigo de periódico biblioteca |
Language: | Ingles English |
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2022-01-28
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Subjects: | Camu-camu, Somatic embryogenesis, Growth regulators, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1139460 |
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dig-alice-doc-11394602022-01-29T02:07:34Z Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant. ARAÚJO, M. da C. da R. CHAGAS, E. A. VENDRAME, W. RIBEIRO, M. I. G. MOURA, E. A. de TAVEIRA, D. L. L. CHAGAS, P. C. GRIGIO, M. L. EDVAN ALVES CHAGAS, CPAF-RR. Camu-camu Somatic embryogenesis Growth regulators Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to have the highest vitamin C content of any edible fruit (13.757 mg 100 g-1 dry weight). The objective of this study was to evaluate the effects of 2,4-D and 6-BAP alone or in combination on in vitro callus induction and pro-embryogenic mass formation in caçari stem segments in different culture media as a precursor for caçari micropropagation. The experiment consisted of two culture media (MS and WPM) and combinations of four concentrations of 2,4-D (0, 1, 2 and 4 mg L-1) and BAP (0, 0.25, 0.5 and 1 mg L-1). The results showed that the highest percentage of callus formation (99%) was obtained in WPM supplemented with 4 mg L-1, 2,4-D and 1 mg L-1 BAP, and this combination resulted in 93% pro-embryogenic mass formation. 2022-01-29T02:07:24Z 2022-01-29T02:07:24Z 2022-01-28 2021 Artigo de periódico Crop Breeding and Applied Biotechnology, v. 21, N. 3, 2021. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1139460 Ingles en openAccess |
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Camu-camu Somatic embryogenesis Growth regulators Camu-camu Somatic embryogenesis Growth regulators |
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Camu-camu Somatic embryogenesis Growth regulators Camu-camu Somatic embryogenesis Growth regulators ARAÚJO, M. da C. da R. CHAGAS, E. A. VENDRAME, W. RIBEIRO, M. I. G. MOURA, E. A. de TAVEIRA, D. L. L. CHAGAS, P. C. GRIGIO, M. L. Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant. |
description |
Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to have the highest vitamin C content of any edible fruit (13.757 mg 100 g-1 dry weight). The objective of this study was to evaluate the effects of 2,4-D and 6-BAP alone or in combination on in vitro callus induction and pro-embryogenic mass formation in caçari stem segments in different culture media as a precursor for caçari micropropagation. The experiment consisted of two culture media (MS and WPM) and combinations of four concentrations of 2,4-D (0, 1, 2 and 4 mg L-1) and BAP (0, 0.25, 0.5 and 1 mg L-1). The results showed that the highest percentage of callus formation (99%) was obtained in WPM supplemented with 4 mg L-1, 2,4-D and 1 mg L-1 BAP, and this combination resulted in 93% pro-embryogenic mass formation. |
author2 |
EDVAN ALVES CHAGAS, CPAF-RR. |
author_facet |
EDVAN ALVES CHAGAS, CPAF-RR. ARAÚJO, M. da C. da R. CHAGAS, E. A. VENDRAME, W. RIBEIRO, M. I. G. MOURA, E. A. de TAVEIRA, D. L. L. CHAGAS, P. C. GRIGIO, M. L. |
format |
Artigo de periódico |
topic_facet |
Camu-camu Somatic embryogenesis Growth regulators |
author |
ARAÚJO, M. da C. da R. CHAGAS, E. A. VENDRAME, W. RIBEIRO, M. I. G. MOURA, E. A. de TAVEIRA, D. L. L. CHAGAS, P. C. GRIGIO, M. L. |
author_sort |
ARAÚJO, M. da C. da R. |
title |
Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant. |
title_short |
Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant. |
title_full |
Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant. |
title_fullStr |
Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant. |
title_full_unstemmed |
Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant. |
title_sort |
callus induction and pro-embryogenic mass formation in myrciaria dubia, an important medicinal and nutritional plant. |
publishDate |
2022-01-28 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1139460 |
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