Karyotype and leaf epidermis histology traits of Digitaria abyssinica (Hochst. Ex A. Rich.) (Poaceae).
Digitaria abyssinica (African couchgrass) has economic potential as forage. This species is taxonomically complex, with a high number of synonyms, basic chromosome number x = 9, and diploid and tetraploid cytotypes. Anatomical, histological, and cytotaxonomic studies may contribute to the taxonomic description of this species and reveal its polyploidization and hybridization events. To this end, this study aimed to characterize the karyotype and leaf epidermis histology of two D. abyssinica genotypes. Chromosome characterization was performed based on chromosome banding using chromomycin A (CMA) and 4,6-diamidino-2-phenylindole (DAPI) and identification of 35S and 5S rDNA sites by fluorescent in situ hybridization. Nuclear DNA quantification was performed by flow cytometry. Paradermal sections were obtained for the leaf epidermis study by light and electron microscopy. The results confirmed that both genotypes are tetraploid (2n = 36). The number and distribution of 35S and 5S rDNA sites suggest the occurrence of postpolyploidization structural chromosomal changes or hybridization processes. Despite intraspecific variation in the number of 5S rDNA sites and CMA+/DAPI+ bands, no changes were identified in karyotypical symmetry or genome size. Leaf epidermis histology traits and cytogenetic data can support breeding programs and germplasm banks in identifying species or cultivars.
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Format: | Artigo de periódico biblioteca |
Language: | Ingles English |
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2021-08-13
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Subjects: | Caracterização cromossômica, Heterocromatina, Polimorfismo cromossômico, Chromosomal polymorphism, Grama, Forragem, Poliploidia, Citotaxonomia, Polyploidy, Heterochromatin, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133568 https://doi.org/10.2478/s11756-020-00630-8 |
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dig-alice-doc-11335682021-08-14T02:03:16Z Karyotype and leaf epidermis histology traits of Digitaria abyssinica (Hochst. Ex A. Rich.) (Poaceae). CHAVES, A. L. A. CHIAVEGATTO, R. B. FERREIRA, M. T. M. ROCHA, L. C. AZEVEDO, A. L. S. BENITES, F. R. G. TECHIO, V. H. ANA LUISA ARANTES CHAVES, Universidade Federal de Lavras; RAQUEL BEZERRA CHIAVEGATTO, Universidade Federal de Lavras; MARCO TULIO MENDES FERREIRA, Universidade Federal de Lavras; LAIANE CORSINI ROCHA, Universidade Federal de Lavras; ANA LUISA SOUSA AZEVEDO, CNPGL; FLAVIO RODRIGO GANDOLFI BENITES, CNPGL; VÂNIA HELENA TECHIO, Universidade Federal de Lavras. Caracterização cromossômica Heterocromatina Polimorfismo cromossômico Chromosomal polymorphism Grama Forragem Poliploidia Citotaxonomia Polyploidy Heterochromatin Digitaria abyssinica (African couchgrass) has economic potential as forage. This species is taxonomically complex, with a high number of synonyms, basic chromosome number x = 9, and diploid and tetraploid cytotypes. Anatomical, histological, and cytotaxonomic studies may contribute to the taxonomic description of this species and reveal its polyploidization and hybridization events. To this end, this study aimed to characterize the karyotype and leaf epidermis histology of two D. abyssinica genotypes. Chromosome characterization was performed based on chromosome banding using chromomycin A (CMA) and 4,6-diamidino-2-phenylindole (DAPI) and identification of 35S and 5S rDNA sites by fluorescent in situ hybridization. Nuclear DNA quantification was performed by flow cytometry. Paradermal sections were obtained for the leaf epidermis study by light and electron microscopy. The results confirmed that both genotypes are tetraploid (2n = 36). The number and distribution of 35S and 5S rDNA sites suggest the occurrence of postpolyploidization structural chromosomal changes or hybridization processes. Despite intraspecific variation in the number of 5S rDNA sites and CMA+/DAPI+ bands, no changes were identified in karyotypical symmetry or genome size. Leaf epidermis histology traits and cytogenetic data can support breeding programs and germplasm banks in identifying species or cultivars. 2021-08-14T02:03:08Z 2021-08-14T02:03:08Z 2021-08-13 2020 Artigo de periódico Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 72, n. 4, p. 1093-1101, 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133568 https://doi.org/10.2478/s11756-020-00630-8 Ingles en openAccess |
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Caracterização cromossômica Heterocromatina Polimorfismo cromossômico Chromosomal polymorphism Grama Forragem Poliploidia Citotaxonomia Polyploidy Heterochromatin Caracterização cromossômica Heterocromatina Polimorfismo cromossômico Chromosomal polymorphism Grama Forragem Poliploidia Citotaxonomia Polyploidy Heterochromatin |
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Caracterização cromossômica Heterocromatina Polimorfismo cromossômico Chromosomal polymorphism Grama Forragem Poliploidia Citotaxonomia Polyploidy Heterochromatin Caracterização cromossômica Heterocromatina Polimorfismo cromossômico Chromosomal polymorphism Grama Forragem Poliploidia Citotaxonomia Polyploidy Heterochromatin CHAVES, A. L. A. CHIAVEGATTO, R. B. FERREIRA, M. T. M. ROCHA, L. C. AZEVEDO, A. L. S. BENITES, F. R. G. TECHIO, V. H. Karyotype and leaf epidermis histology traits of Digitaria abyssinica (Hochst. Ex A. Rich.) (Poaceae). |
description |
Digitaria abyssinica (African couchgrass) has economic potential as forage. This species is taxonomically complex, with a high number of synonyms, basic chromosome number x = 9, and diploid and tetraploid cytotypes. Anatomical, histological, and cytotaxonomic studies may contribute to the taxonomic description of this species and reveal its polyploidization and hybridization events. To this end, this study aimed to characterize the karyotype and leaf epidermis histology of two D. abyssinica genotypes. Chromosome characterization was performed based on chromosome banding using chromomycin A (CMA) and 4,6-diamidino-2-phenylindole (DAPI) and identification of 35S and 5S rDNA sites by fluorescent in situ hybridization. Nuclear DNA quantification was performed by flow cytometry. Paradermal sections were obtained for the leaf epidermis study by light and electron microscopy. The results confirmed that both genotypes are tetraploid (2n = 36). The number and distribution of 35S and 5S rDNA sites suggest the occurrence of postpolyploidization structural chromosomal changes or hybridization processes. Despite intraspecific variation in the number of 5S rDNA sites and CMA+/DAPI+ bands, no changes were identified in karyotypical symmetry or genome size. Leaf epidermis histology traits and cytogenetic data can support breeding programs and germplasm banks in identifying species or cultivars. |
author2 |
ANA LUISA ARANTES CHAVES, Universidade Federal de Lavras; RAQUEL BEZERRA CHIAVEGATTO, Universidade Federal de Lavras; MARCO TULIO MENDES FERREIRA, Universidade Federal de Lavras; LAIANE CORSINI ROCHA, Universidade Federal de Lavras; ANA LUISA SOUSA AZEVEDO, CNPGL; FLAVIO RODRIGO GANDOLFI BENITES, CNPGL; VÂNIA HELENA TECHIO, Universidade Federal de Lavras. |
author_facet |
ANA LUISA ARANTES CHAVES, Universidade Federal de Lavras; RAQUEL BEZERRA CHIAVEGATTO, Universidade Federal de Lavras; MARCO TULIO MENDES FERREIRA, Universidade Federal de Lavras; LAIANE CORSINI ROCHA, Universidade Federal de Lavras; ANA LUISA SOUSA AZEVEDO, CNPGL; FLAVIO RODRIGO GANDOLFI BENITES, CNPGL; VÂNIA HELENA TECHIO, Universidade Federal de Lavras. CHAVES, A. L. A. CHIAVEGATTO, R. B. FERREIRA, M. T. M. ROCHA, L. C. AZEVEDO, A. L. S. BENITES, F. R. G. TECHIO, V. H. |
format |
Artigo de periódico |
topic_facet |
Caracterização cromossômica Heterocromatina Polimorfismo cromossômico Chromosomal polymorphism Grama Forragem Poliploidia Citotaxonomia Polyploidy Heterochromatin |
author |
CHAVES, A. L. A. CHIAVEGATTO, R. B. FERREIRA, M. T. M. ROCHA, L. C. AZEVEDO, A. L. S. BENITES, F. R. G. TECHIO, V. H. |
author_sort |
CHAVES, A. L. A. |
title |
Karyotype and leaf epidermis histology traits of Digitaria abyssinica (Hochst. Ex A. Rich.) (Poaceae). |
title_short |
Karyotype and leaf epidermis histology traits of Digitaria abyssinica (Hochst. Ex A. Rich.) (Poaceae). |
title_full |
Karyotype and leaf epidermis histology traits of Digitaria abyssinica (Hochst. Ex A. Rich.) (Poaceae). |
title_fullStr |
Karyotype and leaf epidermis histology traits of Digitaria abyssinica (Hochst. Ex A. Rich.) (Poaceae). |
title_full_unstemmed |
Karyotype and leaf epidermis histology traits of Digitaria abyssinica (Hochst. Ex A. Rich.) (Poaceae). |
title_sort |
karyotype and leaf epidermis histology traits of digitaria abyssinica (hochst. ex a. rich.) (poaceae). |
publishDate |
2021-08-13 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133568 https://doi.org/10.2478/s11756-020-00630-8 |
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