First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product.

Global publications on Q fever have increased after the 2007 epidemic in the Netherlands. However, the epidemiology of Q fever/coxiellosis in Brazil is still poorly understood. Accordingly, there have been few studies investigating the presence of Coxiella burnetii in dairy products around the world, especially in Brazil, where consumption of fresh cheese made from raw-milk is very high. Objective: This study was a random survey to assess the prevalence of C. burnetii by PCR in traditional Minas artisanal cheese from the Serro microregion, Brazil, which is manufactured from bovine raw-milk. Methods: DNA extracted from 53 cheese samples were analyzed by nested PCR with C. burnetii-specific primers and the products confirmed by DNA sequencing. Results: Out of the 53 cheese samples five (9.43%) were C. burnetii DNA-positive, each coming from one of the respective randomly selected manufacturing agroindustries. Based on our results, it is estimated that 1.62 tons/day of ready-to-eat cheese made from raw-milk from a total of 16.2 tons produced daily in the study region are contaminated with C. burnetii.

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Auteurs principaux: ROZENTAL, T., FARIA, L. S. de, FORNEAS, D., GUTERRES, A. L., RIBEIRO, J. B., ARAUJO, F. R., LEMOS, E. R. S., SILVA, M. R.
Autres auteurs: TATIANA ROZENTAL, Fundação Oswaldo Cruz
Format: Artigo de periódico biblioteca
Langue:Ingles
English
Publié: 2021-08-09
Sujets:Queijo mineiro artesanal., Segurança Alimentar, Queijo de Minas, Zoonose, Leite., Coxiella burnetii, Food safety,
Accès en ligne:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133335
https://doi.org/10.1016/j.bjid.2020.05.003
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spelling dig-alice-doc-11333352021-08-09T12:00:43Z First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product. ROZENTAL, T. FARIA, L. S. de FORNEAS, D. GUTERRES, A. L. RIBEIRO, J. B. ARAUJO, F. R. LEMOS, E. R. S. SILVA, M. R. TATIANA ROZENTAL, Fundação Oswaldo Cruz ALEXANDRO GUTERRES, Fundação Oswaldo Cruz JOAO BATISTA RIBEIRO, CNPGL LETÍCIA SCAFUTTO DE FARIA, Universidade Federal de Juiz de Fora ELBA REGINA SAMPAIO LEMOS, Fundação Oswaldo Cruz MARCIO ROBERTO SILVA, CNPGL. FLABIO RIBEIRO DE ARAUJO, CNPGC DANIELLE FORNEAS, Fundação Oswaldo Cruz Queijo mineiro artesanal. Segurança Alimentar Queijo de Minas Zoonose Leite. Coxiella burnetii Food safety Global publications on Q fever have increased after the 2007 epidemic in the Netherlands. However, the epidemiology of Q fever/coxiellosis in Brazil is still poorly understood. Accordingly, there have been few studies investigating the presence of Coxiella burnetii in dairy products around the world, especially in Brazil, where consumption of fresh cheese made from raw-milk is very high. Objective: This study was a random survey to assess the prevalence of C. burnetii by PCR in traditional Minas artisanal cheese from the Serro microregion, Brazil, which is manufactured from bovine raw-milk. Methods: DNA extracted from 53 cheese samples were analyzed by nested PCR with C. burnetii-specific primers and the products confirmed by DNA sequencing. Results: Out of the 53 cheese samples five (9.43%) were C. burnetii DNA-positive, each coming from one of the respective randomly selected manufacturing agroindustries. Based on our results, it is estimated that 1.62 tons/day of ready-to-eat cheese made from raw-milk from a total of 16.2 tons produced daily in the study region are contaminated with C. burnetii. 2021-08-09T12:00:32Z 2021-08-09T12:00:32Z 2021-08-09 2020 Artigo de periódico The Brazilian Journal of Infectious Diseases, v. 24, n. 3, p. 208-212, 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133335 https://doi.org/10.1016/j.bjid.2020.05.003 Ingles en openAccess
institution EMBRAPA
collection DSpace
country Brasil
countrycode BR
component Bibliográfico
access En linea
databasecode dig-alice
tag biblioteca
region America del Sur
libraryname Sistema de bibliotecas de EMBRAPA
language Ingles
English
topic Queijo mineiro artesanal.
Segurança Alimentar
Queijo de Minas
Zoonose
Leite.
Coxiella burnetii
Food safety
Queijo mineiro artesanal.
Segurança Alimentar
Queijo de Minas
Zoonose
Leite.
Coxiella burnetii
Food safety
spellingShingle Queijo mineiro artesanal.
Segurança Alimentar
Queijo de Minas
Zoonose
Leite.
Coxiella burnetii
Food safety
Queijo mineiro artesanal.
Segurança Alimentar
Queijo de Minas
Zoonose
Leite.
Coxiella burnetii
Food safety
ROZENTAL, T.
FARIA, L. S. de
FORNEAS, D.
GUTERRES, A. L.
RIBEIRO, J. B.
ARAUJO, F. R.
LEMOS, E. R. S.
SILVA, M. R.
First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product.
description Global publications on Q fever have increased after the 2007 epidemic in the Netherlands. However, the epidemiology of Q fever/coxiellosis in Brazil is still poorly understood. Accordingly, there have been few studies investigating the presence of Coxiella burnetii in dairy products around the world, especially in Brazil, where consumption of fresh cheese made from raw-milk is very high. Objective: This study was a random survey to assess the prevalence of C. burnetii by PCR in traditional Minas artisanal cheese from the Serro microregion, Brazil, which is manufactured from bovine raw-milk. Methods: DNA extracted from 53 cheese samples were analyzed by nested PCR with C. burnetii-specific primers and the products confirmed by DNA sequencing. Results: Out of the 53 cheese samples five (9.43%) were C. burnetii DNA-positive, each coming from one of the respective randomly selected manufacturing agroindustries. Based on our results, it is estimated that 1.62 tons/day of ready-to-eat cheese made from raw-milk from a total of 16.2 tons produced daily in the study region are contaminated with C. burnetii.
author2 TATIANA ROZENTAL, Fundação Oswaldo Cruz
author_facet TATIANA ROZENTAL, Fundação Oswaldo Cruz
ROZENTAL, T.
FARIA, L. S. de
FORNEAS, D.
GUTERRES, A. L.
RIBEIRO, J. B.
ARAUJO, F. R.
LEMOS, E. R. S.
SILVA, M. R.
format Artigo de periódico
topic_facet Queijo mineiro artesanal.
Segurança Alimentar
Queijo de Minas
Zoonose
Leite.
Coxiella burnetii
Food safety
author ROZENTAL, T.
FARIA, L. S. de
FORNEAS, D.
GUTERRES, A. L.
RIBEIRO, J. B.
ARAUJO, F. R.
LEMOS, E. R. S.
SILVA, M. R.
author_sort ROZENTAL, T.
title First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product.
title_short First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product.
title_full First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product.
title_fullStr First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product.
title_full_unstemmed First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product.
title_sort first molecular detection of coxiella burnetii in brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product.
publishDate 2021-08-09
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133335
https://doi.org/10.1016/j.bjid.2020.05.003
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