First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product.
Global publications on Q fever have increased after the 2007 epidemic in the Netherlands. However, the epidemiology of Q fever/coxiellosis in Brazil is still poorly understood. Accordingly, there have been few studies investigating the presence of Coxiella burnetii in dairy products around the world, especially in Brazil, where consumption of fresh cheese made from raw-milk is very high. Objective: This study was a random survey to assess the prevalence of C. burnetii by PCR in traditional Minas artisanal cheese from the Serro microregion, Brazil, which is manufactured from bovine raw-milk. Methods: DNA extracted from 53 cheese samples were analyzed by nested PCR with C. burnetii-specific primers and the products confirmed by DNA sequencing. Results: Out of the 53 cheese samples five (9.43%) were C. burnetii DNA-positive, each coming from one of the respective randomly selected manufacturing agroindustries. Based on our results, it is estimated that 1.62 tons/day of ready-to-eat cheese made from raw-milk from a total of 16.2 tons produced daily in the study region are contaminated with C. burnetii.
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Format: | Artigo de periódico biblioteca |
Langue: | Ingles English |
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2021-08-09
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Sujets: | Queijo mineiro artesanal., Segurança Alimentar, Queijo de Minas, Zoonose, Leite., Coxiella burnetii, Food safety, |
Accès en ligne: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133335 https://doi.org/10.1016/j.bjid.2020.05.003 |
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dig-alice-doc-11333352021-08-09T12:00:43Z First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product. ROZENTAL, T. FARIA, L. S. de FORNEAS, D. GUTERRES, A. L. RIBEIRO, J. B. ARAUJO, F. R. LEMOS, E. R. S. SILVA, M. R. TATIANA ROZENTAL, Fundação Oswaldo Cruz ALEXANDRO GUTERRES, Fundação Oswaldo Cruz JOAO BATISTA RIBEIRO, CNPGL LETÍCIA SCAFUTTO DE FARIA, Universidade Federal de Juiz de Fora ELBA REGINA SAMPAIO LEMOS, Fundação Oswaldo Cruz MARCIO ROBERTO SILVA, CNPGL. FLABIO RIBEIRO DE ARAUJO, CNPGC DANIELLE FORNEAS, Fundação Oswaldo Cruz Queijo mineiro artesanal. Segurança Alimentar Queijo de Minas Zoonose Leite. Coxiella burnetii Food safety Global publications on Q fever have increased after the 2007 epidemic in the Netherlands. However, the epidemiology of Q fever/coxiellosis in Brazil is still poorly understood. Accordingly, there have been few studies investigating the presence of Coxiella burnetii in dairy products around the world, especially in Brazil, where consumption of fresh cheese made from raw-milk is very high. Objective: This study was a random survey to assess the prevalence of C. burnetii by PCR in traditional Minas artisanal cheese from the Serro microregion, Brazil, which is manufactured from bovine raw-milk. Methods: DNA extracted from 53 cheese samples were analyzed by nested PCR with C. burnetii-specific primers and the products confirmed by DNA sequencing. Results: Out of the 53 cheese samples five (9.43%) were C. burnetii DNA-positive, each coming from one of the respective randomly selected manufacturing agroindustries. Based on our results, it is estimated that 1.62 tons/day of ready-to-eat cheese made from raw-milk from a total of 16.2 tons produced daily in the study region are contaminated with C. burnetii. 2021-08-09T12:00:32Z 2021-08-09T12:00:32Z 2021-08-09 2020 Artigo de periódico The Brazilian Journal of Infectious Diseases, v. 24, n. 3, p. 208-212, 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133335 https://doi.org/10.1016/j.bjid.2020.05.003 Ingles en openAccess |
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Queijo mineiro artesanal. Segurança Alimentar Queijo de Minas Zoonose Leite. Coxiella burnetii Food safety Queijo mineiro artesanal. Segurança Alimentar Queijo de Minas Zoonose Leite. Coxiella burnetii Food safety |
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Queijo mineiro artesanal. Segurança Alimentar Queijo de Minas Zoonose Leite. Coxiella burnetii Food safety Queijo mineiro artesanal. Segurança Alimentar Queijo de Minas Zoonose Leite. Coxiella burnetii Food safety ROZENTAL, T. FARIA, L. S. de FORNEAS, D. GUTERRES, A. L. RIBEIRO, J. B. ARAUJO, F. R. LEMOS, E. R. S. SILVA, M. R. First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product. |
description |
Global publications on Q fever have increased after the 2007 epidemic in the Netherlands. However, the epidemiology of Q fever/coxiellosis in Brazil is still poorly understood. Accordingly, there have been few studies investigating the presence of Coxiella burnetii in dairy products around the world, especially in Brazil, where consumption of fresh cheese made from raw-milk is very high. Objective: This study was a random survey to assess the prevalence of C. burnetii by PCR in traditional Minas artisanal cheese from the Serro microregion, Brazil, which is manufactured from bovine raw-milk. Methods: DNA extracted from 53 cheese samples were analyzed by nested PCR with C. burnetii-specific primers and the products confirmed by DNA sequencing. Results: Out of the 53 cheese samples five (9.43%) were C. burnetii DNA-positive, each coming from one of the respective randomly selected manufacturing agroindustries. Based on our results, it is estimated that 1.62 tons/day of ready-to-eat cheese made from raw-milk from a total of 16.2 tons produced daily in the study region are contaminated with C. burnetii. |
author2 |
TATIANA ROZENTAL, Fundação Oswaldo Cruz |
author_facet |
TATIANA ROZENTAL, Fundação Oswaldo Cruz ROZENTAL, T. FARIA, L. S. de FORNEAS, D. GUTERRES, A. L. RIBEIRO, J. B. ARAUJO, F. R. LEMOS, E. R. S. SILVA, M. R. |
format |
Artigo de periódico |
topic_facet |
Queijo mineiro artesanal. Segurança Alimentar Queijo de Minas Zoonose Leite. Coxiella burnetii Food safety |
author |
ROZENTAL, T. FARIA, L. S. de FORNEAS, D. GUTERRES, A. L. RIBEIRO, J. B. ARAUJO, F. R. LEMOS, E. R. S. SILVA, M. R. |
author_sort |
ROZENTAL, T. |
title |
First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product. |
title_short |
First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product. |
title_full |
First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product. |
title_fullStr |
First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product. |
title_full_unstemmed |
First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product. |
title_sort |
first molecular detection of coxiella burnetii in brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product. |
publishDate |
2021-08-09 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133335 https://doi.org/10.1016/j.bjid.2020.05.003 |
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