Genome-wide association study for resistance to the Meloidogyne javanica causing root-knot nematode in soybean.

Key message A locus on chromosome 13, containing multiple TIR-NB-LRR genes and SNPs associated with M. javanica resistance, was identified using a combination of GWAS, resequencing, genetic mapping and expression profiling. Abstract Meloidogyne javanica, a root-knot nematode, is an important problem in soybean-growing areas, leading to severe yield losses. Some accessions have been identified carrying resistance loci to this nematode. In this study, a set of 317 soybean accessions was characterized for resistance to M. javanica. A genome-wide association study was performed using SNPs from genotyping-by-sequencing, and a region of 29.2 kb on chromosome 13 was identified. An analysis of haplotypes showed that SNPs were able to discriminate between susceptible and resistant accessions, with 25 accessions sharing the haplotype associated with resistance. Furthermore, five accessions that exhibited resistance without carrying this haplotype may carry different loci conferring resistance to M. javanica. We also conducted the screening of the SNPs in the USDA soybean germplasm, revealing that several soybean accessions previously reported as resistant to other nematodes also shared the resistance haplotype on chromosome 13. Two SNP-based TaqMan® assays were developed and validated in two panels of soybean cultivars and in biparental populations. In silico analysis of the region associated with resistance identified the occurrence of genes with structural similarity with classical major resistance genes (NBS-LRR genes). Specifically, several nonsynonymous SNPs were observed in Glyma.13g194800 and Glyma.13g194900. The expression profile of these candidate genes demonstrated that the two gene models were up-regulated in the resistance source PI 505,099 after nematode infection. Overall, the SNPs associated with resistance and the genes identified constitute an important tool for introgression of resistance to the root-knot nematode by marker-assisted selection in soybean breeding programs.

Bibliographic Details

Main Authors:	ALEKCEVETCH, J. C., PASSIANOTTO, A. L. de L., FERREIRA, E. G. C., SANTOS, A. B. dos, SILVA, D. C. G. da, DIAS, W. P., BELZILE, F., ABDELNOOR, R. V., MARCELINO-GUIMARÃES, F. C.
Other Authors:	JEAN CARLOS ALEKCEVETCH, Universidade Estadual de Londrina, UEL, Londrina, PR.; ANDRÉ LUIZ DE LIMA PASSIANOTTO, University of Guelph, Guelph, Canada.; EVERTON GERALDO CAPOTE FERREIRA, Universidade Estadual de Londrina, UEL, Londrina, PR.; ADRIANA BROMBINI DOS SANTOS, CNPSO; DANIELLE CRISTINA GREGORIO DA SILVA, CNPSO; WALDIR PEREIRA DIAS, CNPSO; FRANÇOIS BELZILE, Université Laval, Quebec City, Quebec , Canada.; RICARDO VILELA ABDELNOOR, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARA, CNPSO.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2021-03-24
Subjects:	Soja, Genoma, Resistência, Meloidogyne Javanica, Nematóide, Galha, Genome-wide association study, Resistance mechanisms, Root-knot nematodes, Soybeans,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130887 https://doi.org/10.1007/s00122-020-03723-9
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