Major effect qtl on chromosome 3 conferring maize resistance to Sugarcane mosaic virus.
The Sugarcane mosaic virus (SCMV), a maize pathogen epidemic worldwide, is the causal agent of common mosaic, one of the most important viral diseases in Brazil. In this study, we mapped and characterized quantitative trait loci (QTL) conferring resistance to SCMV in a maize population consisting of 127 F2:3 families from the cross between two Brazilian maize inbred lines, L18 (resistant) × L19 (susceptible). Field trials were carried out in two years to evaluate the F2:3 families according to a resistance score after artificial inoculation. QTLs were detected via composite interval mapping, using a linkage map based on 82 SSRs, 3 CAPS and 296 SNPs. The heritability ranged from 73.68 to 95.16% and SCMV resistance QTLs were consistently identified on chromosomes 1 and 3, showing minor and major effects, respectively. The major QTL on chromosome 3 explained a large proportion of the genetic variance, being 50 and 70% in year 1 and 2, respectively, while the minor QTL on chromosome 1 explained 11 and 8% in year 1 and 2, respectively. The SNP marker co-localized with the major QTL peak on chromosome 3 and its right flanking marker are positioned inside the predicted gene GRMZM2G122443 encoding a glucosidase II, and the left flanking marker inside the GRMZM2G140537 that encodes a protein tyrosine kinase. Moreover, within this QTL region there are also the GRMZM2G160902 and GRMZM2G122481 predicted genes, encoding a bZIP transcription factor and a cytochrome C oxidase, respectively. The colocalization with this major effect QTL suggests a putative involvement of these candidate genes with maize responses to SCMV resistance, but further functional studies are required for such validation. Our results provide resistance source and genomic target for marker-assisted breeding aiming the development of maize resistant cultivars to SCMV.
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| Idioma: | English eng |
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2020-01-24
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| Materias: | Zea Mays, Marcador Genético, Gene, Linhagem, |
| Acceso en línea: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1119299 |
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dig-alice-doc-11192992020-01-24T18:14:55Z Major effect qtl on chromosome 3 conferring maize resistance to Sugarcane mosaic virus. SOUZA, I. R. P. de GUILHEN, J. H. S. ANDRADE, C. de L. T. de PINTO, M. de O. LANA, U. G. de P. PASTINA, M. M. ISABEL REGINA PRAZERES DE SOUZA, CNPMS; JOSÉ HENRIQUE SOLER GUILHEN, Universidade Federal do Espírito Santo; CAMILO DE LELIS TEIXEIRA DE ANDRADE, CNPMS; MARCOS DE OLIVEIRA PINTO, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; MARIA MARTA PASTINA, CNPMS. Zea Mays Marcador Genético Gene Linhagem The Sugarcane mosaic virus (SCMV), a maize pathogen epidemic worldwide, is the causal agent of common mosaic, one of the most important viral diseases in Brazil. In this study, we mapped and characterized quantitative trait loci (QTL) conferring resistance to SCMV in a maize population consisting of 127 F2:3 families from the cross between two Brazilian maize inbred lines, L18 (resistant) × L19 (susceptible). Field trials were carried out in two years to evaluate the F2:3 families according to a resistance score after artificial inoculation. QTLs were detected via composite interval mapping, using a linkage map based on 82 SSRs, 3 CAPS and 296 SNPs. The heritability ranged from 73.68 to 95.16% and SCMV resistance QTLs were consistently identified on chromosomes 1 and 3, showing minor and major effects, respectively. The major QTL on chromosome 3 explained a large proportion of the genetic variance, being 50 and 70% in year 1 and 2, respectively, while the minor QTL on chromosome 1 explained 11 and 8% in year 1 and 2, respectively. The SNP marker co-localized with the major QTL peak on chromosome 3 and its right flanking marker are positioned inside the predicted gene GRMZM2G122443 encoding a glucosidase II, and the left flanking marker inside the GRMZM2G140537 that encodes a protein tyrosine kinase. Moreover, within this QTL region there are also the GRMZM2G160902 and GRMZM2G122481 predicted genes, encoding a bZIP transcription factor and a cytochrome C oxidase, respectively. The colocalization with this major effect QTL suggests a putative involvement of these candidate genes with maize responses to SCMV resistance, but further functional studies are required for such validation. Our results provide resistance source and genomic target for marker-assisted breeding aiming the development of maize resistant cultivars to SCMV. 2020-01-24T18:14:49Z 2020-01-24T18:14:49Z 2020-01-24 2019 2020-01-24T18:14:49Z Artigo de periódico Revista Brasileira de Milho e Sorgo, v. 18, n. 3, p. 322-339, 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1119299 en eng openAccess |
| institution |
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| country |
Brasil |
| countrycode |
BR |
| component |
Bibliográfico |
| access |
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| databasecode |
dig-alice |
| tag |
biblioteca |
| region |
America del Sur |
| libraryname |
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| language |
English eng |
| topic |
Zea Mays Marcador Genético Gene Linhagem Zea Mays Marcador Genético Gene Linhagem |
| spellingShingle |
Zea Mays Marcador Genético Gene Linhagem Zea Mays Marcador Genético Gene Linhagem SOUZA, I. R. P. de GUILHEN, J. H. S. ANDRADE, C. de L. T. de PINTO, M. de O. LANA, U. G. de P. PASTINA, M. M. Major effect qtl on chromosome 3 conferring maize resistance to Sugarcane mosaic virus. |
| description |
The Sugarcane mosaic virus (SCMV), a maize pathogen epidemic worldwide, is the causal agent of common mosaic, one of the most important viral diseases in Brazil. In this study, we mapped and characterized quantitative trait loci (QTL) conferring resistance to SCMV in a maize population consisting of 127 F2:3 families from the cross between two Brazilian maize inbred lines, L18 (resistant) × L19 (susceptible). Field trials were carried out in two years to evaluate the F2:3 families according to a resistance score after artificial inoculation. QTLs were detected via composite interval mapping, using a linkage map based on 82 SSRs, 3 CAPS and 296 SNPs. The heritability ranged from 73.68 to 95.16% and SCMV resistance QTLs were consistently identified on chromosomes 1 and 3, showing minor and major effects, respectively. The major QTL on chromosome 3 explained a large proportion of the genetic variance, being 50 and 70% in year 1 and 2, respectively, while the minor QTL on chromosome 1 explained 11 and 8% in year 1 and 2, respectively. The SNP marker co-localized with the major QTL peak on chromosome 3 and its right flanking marker are positioned inside the predicted gene GRMZM2G122443 encoding a glucosidase II, and the left flanking marker inside the GRMZM2G140537 that encodes a protein tyrosine kinase. Moreover, within this QTL region there are also the GRMZM2G160902 and GRMZM2G122481 predicted genes, encoding a bZIP transcription factor and a cytochrome C oxidase, respectively. The colocalization with this major effect QTL suggests a putative involvement of these candidate genes with maize responses to SCMV resistance, but further functional studies are required for such validation. Our results provide resistance source and genomic target for marker-assisted breeding aiming the development of maize resistant cultivars to SCMV. |
| author2 |
ISABEL REGINA PRAZERES DE SOUZA, CNPMS; JOSÉ HENRIQUE SOLER GUILHEN, Universidade Federal do Espírito Santo; CAMILO DE LELIS TEIXEIRA DE ANDRADE, CNPMS; MARCOS DE OLIVEIRA PINTO, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; MARIA MARTA PASTINA, CNPMS. |
| author_facet |
ISABEL REGINA PRAZERES DE SOUZA, CNPMS; JOSÉ HENRIQUE SOLER GUILHEN, Universidade Federal do Espírito Santo; CAMILO DE LELIS TEIXEIRA DE ANDRADE, CNPMS; MARCOS DE OLIVEIRA PINTO, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; MARIA MARTA PASTINA, CNPMS. SOUZA, I. R. P. de GUILHEN, J. H. S. ANDRADE, C. de L. T. de PINTO, M. de O. LANA, U. G. de P. PASTINA, M. M. |
| format |
Artigo de periódico |
| topic_facet |
Zea Mays Marcador Genético Gene Linhagem |
| author |
SOUZA, I. R. P. de GUILHEN, J. H. S. ANDRADE, C. de L. T. de PINTO, M. de O. LANA, U. G. de P. PASTINA, M. M. |
| author_sort |
SOUZA, I. R. P. de |
| title |
Major effect qtl on chromosome 3 conferring maize resistance to Sugarcane mosaic virus. |
| title_short |
Major effect qtl on chromosome 3 conferring maize resistance to Sugarcane mosaic virus. |
| title_full |
Major effect qtl on chromosome 3 conferring maize resistance to Sugarcane mosaic virus. |
| title_fullStr |
Major effect qtl on chromosome 3 conferring maize resistance to Sugarcane mosaic virus. |
| title_full_unstemmed |
Major effect qtl on chromosome 3 conferring maize resistance to Sugarcane mosaic virus. |
| title_sort |
major effect qtl on chromosome 3 conferring maize resistance to sugarcane mosaic virus. |
| publishDate |
2020-01-24 |
| url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1119299 |
| work_keys_str_mv |
AT souzairpde majoreffectqtlonchromosome3conferringmaizeresistancetosugarcanemosaicvirus AT guilhenjhs majoreffectqtlonchromosome3conferringmaizeresistancetosugarcanemosaicvirus AT andradecdeltde majoreffectqtlonchromosome3conferringmaizeresistancetosugarcanemosaicvirus AT pintomdeo majoreffectqtlonchromosome3conferringmaizeresistancetosugarcanemosaicvirus AT lanaugdep majoreffectqtlonchromosome3conferringmaizeresistancetosugarcanemosaicvirus AT pastinamm majoreffectqtlonchromosome3conferringmaizeresistancetosugarcanemosaicvirus |
| _version_ |
1756026770896715776 |