Bio compounds of edible mushrooms: in vitro antioxidant and antimicrobial activities.
The aim of this study was to optimize the extraction of total phenolics from edible mushrooms, evaluate the in vitro antioxidant and antimicrobial activities and identify the main phenolic compounds present in the extracts. A Box-Behnken design was used and the effects of temperature (X1, 25?55 °C), solvent-to-solid ratio (X2, 30?70 mL per gram) and solvent concentration (X3, 25?75%) were evaluated. In the optimum conditions of extraction, the antioxidant (DPPH, ABTS and FRAP assays) and antimicrobial activities of the extract were tested against the bacteria: Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella enteritidis. In addition, the phenolic compounds of the extracts were quantified. The A. brasiliensis mushrooms showed the higher phenolic contents (13.16 mgGAE/g) and antioxidant activity by DPPH and ABTS assays of 50.64 and 128.60 μmolTE/g, respectively, among the phenolic extracts of the mushrooms analyzed. The gallic acid was the main phenolic compound identified and the A. brasiliensis had the highest concentration (491.89 μg/g). All extracts presented antibacterial activity for Gram-positive strains (MIC ≤ 200 mg/mL). The high content of antioxidant compounds, extracted by a non-toxic solvent, suggested that the A. brasiliensis extract can be applied in the food industry as a natural antioxidant.
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| Autores principales: | , , , , , , , |
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| Formato: | Artigo de periódico biblioteca |
| Idioma: | Ingles English |
| Publicado: |
2019-10-24
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| Materias: | Agaricus brasiliensis, UHPLC, Phenolic acid, Antibacterial activity, Atividade antibacteriana, Ácido fenólico, Edible mushrooms, Cogumelo Comestível, Gallic acid, |
| Acceso en línea: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113459 |
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dig-alice-doc-11134592022-05-20T11:13:37Z Bio compounds of edible mushrooms: in vitro antioxidant and antimicrobial activities. BACH, F. ZIELINSKI, A. A. HELM, C. V. MACIEL, G. M. PEDRO, A. C. STAFUSSA, A. P. ÁVILA, S. HAMINIUK, C. H. I. Fabiane Bach, UFPR; Acácio Antonio Ferreira Zielinski, UFSC; CRISTIANE VIEIRA HELM, CNPF; Giselle Maria Maciel, UTFPR; Alessandra Cristina Pedro, UFPR; Ana Paula Stafussa, UFPR; Suelen Ávila, UFPR; Charles Windson Isidoro Haminiuk, UTFPR. Agaricus brasiliensis UHPLC Phenolic acid Antibacterial activity Atividade antibacteriana Ácido fenólico Edible mushrooms Cogumelo Comestível Gallic acid The aim of this study was to optimize the extraction of total phenolics from edible mushrooms, evaluate the in vitro antioxidant and antimicrobial activities and identify the main phenolic compounds present in the extracts. A Box-Behnken design was used and the effects of temperature (X1, 25?55 °C), solvent-to-solid ratio (X2, 30?70 mL per gram) and solvent concentration (X3, 25?75%) were evaluated. In the optimum conditions of extraction, the antioxidant (DPPH, ABTS and FRAP assays) and antimicrobial activities of the extract were tested against the bacteria: Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella enteritidis. In addition, the phenolic compounds of the extracts were quantified. The A. brasiliensis mushrooms showed the higher phenolic contents (13.16 mgGAE/g) and antioxidant activity by DPPH and ABTS assays of 50.64 and 128.60 μmolTE/g, respectively, among the phenolic extracts of the mushrooms analyzed. The gallic acid was the main phenolic compound identified and the A. brasiliensis had the highest concentration (491.89 μg/g). All extracts presented antibacterial activity for Gram-positive strains (MIC ≤ 200 mg/mL). The high content of antioxidant compounds, extracted by a non-toxic solvent, suggested that the A. brasiliensis extract can be applied in the food industry as a natural antioxidant. 2022-05-20T11:13:27Z 2022-05-20T11:13:27Z 2019-10-24 2019 Artigo de periódico LWT. Food Science and Technology, v. 107, p. 214-220, June 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113459 10.1016/j.lwt.2019.03.017 Ingles en openAccess |
| institution |
EMBRAPA |
| collection |
DSpace |
| country |
Brasil |
| countrycode |
BR |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-alice |
| tag |
biblioteca |
| region |
America del Sur |
| libraryname |
Sistema de bibliotecas de EMBRAPA |
| language |
Ingles English |
| topic |
Agaricus brasiliensis UHPLC Phenolic acid Antibacterial activity Atividade antibacteriana Ácido fenólico Edible mushrooms Cogumelo Comestível Gallic acid Agaricus brasiliensis UHPLC Phenolic acid Antibacterial activity Atividade antibacteriana Ácido fenólico Edible mushrooms Cogumelo Comestível Gallic acid |
| spellingShingle |
Agaricus brasiliensis UHPLC Phenolic acid Antibacterial activity Atividade antibacteriana Ácido fenólico Edible mushrooms Cogumelo Comestível Gallic acid Agaricus brasiliensis UHPLC Phenolic acid Antibacterial activity Atividade antibacteriana Ácido fenólico Edible mushrooms Cogumelo Comestível Gallic acid BACH, F. ZIELINSKI, A. A. HELM, C. V. MACIEL, G. M. PEDRO, A. C. STAFUSSA, A. P. ÁVILA, S. HAMINIUK, C. H. I. Bio compounds of edible mushrooms: in vitro antioxidant and antimicrobial activities. |
| description |
The aim of this study was to optimize the extraction of total phenolics from edible mushrooms, evaluate the in vitro antioxidant and antimicrobial activities and identify the main phenolic compounds present in the extracts. A Box-Behnken design was used and the effects of temperature (X1, 25?55 °C), solvent-to-solid ratio (X2, 30?70 mL per gram) and solvent concentration (X3, 25?75%) were evaluated. In the optimum conditions of extraction, the antioxidant (DPPH, ABTS and FRAP assays) and antimicrobial activities of the extract were tested against the bacteria: Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella enteritidis. In addition, the phenolic compounds of the extracts were quantified. The A. brasiliensis mushrooms showed the higher phenolic contents (13.16 mgGAE/g) and antioxidant activity by DPPH and ABTS assays of 50.64 and 128.60 μmolTE/g, respectively, among the phenolic extracts of the mushrooms analyzed. The gallic acid was the main phenolic compound identified and the A. brasiliensis had the highest concentration (491.89 μg/g). All extracts presented antibacterial activity for Gram-positive strains (MIC ≤ 200 mg/mL). The high content of antioxidant compounds, extracted by a non-toxic solvent, suggested that the A. brasiliensis extract can be applied in the food industry as a natural antioxidant. |
| author2 |
Fabiane Bach, UFPR; Acácio Antonio Ferreira Zielinski, UFSC; CRISTIANE VIEIRA HELM, CNPF; Giselle Maria Maciel, UTFPR; Alessandra Cristina Pedro, UFPR; Ana Paula Stafussa, UFPR; Suelen Ávila, UFPR; Charles Windson Isidoro Haminiuk, UTFPR. |
| author_facet |
Fabiane Bach, UFPR; Acácio Antonio Ferreira Zielinski, UFSC; CRISTIANE VIEIRA HELM, CNPF; Giselle Maria Maciel, UTFPR; Alessandra Cristina Pedro, UFPR; Ana Paula Stafussa, UFPR; Suelen Ávila, UFPR; Charles Windson Isidoro Haminiuk, UTFPR. BACH, F. ZIELINSKI, A. A. HELM, C. V. MACIEL, G. M. PEDRO, A. C. STAFUSSA, A. P. ÁVILA, S. HAMINIUK, C. H. I. |
| format |
Artigo de periódico |
| topic_facet |
Agaricus brasiliensis UHPLC Phenolic acid Antibacterial activity Atividade antibacteriana Ácido fenólico Edible mushrooms Cogumelo Comestível Gallic acid |
| author |
BACH, F. ZIELINSKI, A. A. HELM, C. V. MACIEL, G. M. PEDRO, A. C. STAFUSSA, A. P. ÁVILA, S. HAMINIUK, C. H. I. |
| author_sort |
BACH, F. |
| title |
Bio compounds of edible mushrooms: in vitro antioxidant and antimicrobial activities. |
| title_short |
Bio compounds of edible mushrooms: in vitro antioxidant and antimicrobial activities. |
| title_full |
Bio compounds of edible mushrooms: in vitro antioxidant and antimicrobial activities. |
| title_fullStr |
Bio compounds of edible mushrooms: in vitro antioxidant and antimicrobial activities. |
| title_full_unstemmed |
Bio compounds of edible mushrooms: in vitro antioxidant and antimicrobial activities. |
| title_sort |
bio compounds of edible mushrooms: in vitro antioxidant and antimicrobial activities. |
| publishDate |
2019-10-24 |
| url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113459 |
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| _version_ |
1756028295583891456 |