Cryopreservation in Coffea canephora Pierre seeds: slow and fast cooling.
Coffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Catalase and esterase enzymes are good biochemical markers for cryopreserved coffee seeds and their activity is greater in larger seed physiological quality.
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Format: | Artigo de periódico biblioteca |
Language: | pt_BR por |
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2019-05-06
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Subjects: | Drying in silica gel, Ice crystals, Isoenzymes, Physiological quality, Secagem em sílica gel, Semente recalcitrante, Cristal de gelo, Qualidade fisiológica, Secagem, Isoenzima, Recalcitrant seeds, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108759 |
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dig-alice-doc-11087592019-05-07T00:50:22Z Cryopreservation in Coffea canephora Pierre seeds: slow and fast cooling. COELHO, S. V. B. ROSA, S. D. V. F. da FANTAZZINI, T. B. BAUTE, J. L. SILVA, L. C. Stefânia Vilas Boas Coelho, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; Tatiana Botelho Fantazzini, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Júlia Lima Baute, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Luciano Coutinho Silva, Departamento de Biologia Celular e Molecular/Universidade Federal da Paraíba - UFPB. Drying in silica gel Ice crystals Isoenzymes Physiological quality Secagem em sílica gel Semente recalcitrante Cristal de gelo Qualidade fisiológica Secagem Isoenzima Recalcitrant seeds Coffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Catalase and esterase enzymes are good biochemical markers for cryopreserved coffee seeds and their activity is greater in larger seed physiological quality. Título em português Criopreservação em sementes de Coffea canephora Pierre: Resfriamento lento e rápido. 2019-05-07T00:50:14Z 2019-05-07T00:50:14Z 2019-05-06 2018 2019-05-07T00:50:14Z Artigo de periódico Ciência e Agrotecnologia, v. 42, n. 6, p. 588-597, Nov/Dec. 2018 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108759 pt_BR por openAccess |
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Drying in silica gel Ice crystals Isoenzymes Physiological quality Secagem em sílica gel Semente recalcitrante Cristal de gelo Qualidade fisiológica Secagem Isoenzima Recalcitrant seeds Drying in silica gel Ice crystals Isoenzymes Physiological quality Secagem em sílica gel Semente recalcitrante Cristal de gelo Qualidade fisiológica Secagem Isoenzima Recalcitrant seeds |
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Drying in silica gel Ice crystals Isoenzymes Physiological quality Secagem em sílica gel Semente recalcitrante Cristal de gelo Qualidade fisiológica Secagem Isoenzima Recalcitrant seeds Drying in silica gel Ice crystals Isoenzymes Physiological quality Secagem em sílica gel Semente recalcitrante Cristal de gelo Qualidade fisiológica Secagem Isoenzima Recalcitrant seeds COELHO, S. V. B. ROSA, S. D. V. F. da FANTAZZINI, T. B. BAUTE, J. L. SILVA, L. C. Cryopreservation in Coffea canephora Pierre seeds: slow and fast cooling. |
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Coffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Catalase and esterase enzymes are good biochemical markers for cryopreserved coffee seeds and their activity is greater in larger seed physiological quality. |
author2 |
Stefânia Vilas Boas Coelho, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; Tatiana Botelho Fantazzini, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Júlia Lima Baute, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Luciano Coutinho Silva, Departamento de Biologia Celular e Molecular/Universidade Federal da Paraíba - UFPB. |
author_facet |
Stefânia Vilas Boas Coelho, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; Tatiana Botelho Fantazzini, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Júlia Lima Baute, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Luciano Coutinho Silva, Departamento de Biologia Celular e Molecular/Universidade Federal da Paraíba - UFPB. COELHO, S. V. B. ROSA, S. D. V. F. da FANTAZZINI, T. B. BAUTE, J. L. SILVA, L. C. |
format |
Artigo de periódico |
topic_facet |
Drying in silica gel Ice crystals Isoenzymes Physiological quality Secagem em sílica gel Semente recalcitrante Cristal de gelo Qualidade fisiológica Secagem Isoenzima Recalcitrant seeds |
author |
COELHO, S. V. B. ROSA, S. D. V. F. da FANTAZZINI, T. B. BAUTE, J. L. SILVA, L. C. |
author_sort |
COELHO, S. V. B. |
title |
Cryopreservation in Coffea canephora Pierre seeds: slow and fast cooling. |
title_short |
Cryopreservation in Coffea canephora Pierre seeds: slow and fast cooling. |
title_full |
Cryopreservation in Coffea canephora Pierre seeds: slow and fast cooling. |
title_fullStr |
Cryopreservation in Coffea canephora Pierre seeds: slow and fast cooling. |
title_full_unstemmed |
Cryopreservation in Coffea canephora Pierre seeds: slow and fast cooling. |
title_sort |
cryopreservation in coffea canephora pierre seeds: slow and fast cooling. |
publishDate |
2019-05-06 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108759 |
work_keys_str_mv |
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_version_ |
1756025931042914304 |