Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach.
Abstract: The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the tillage system, with the NT showing about five times more hydrolases than the CT system. The outstanding differences can be attributed to the management of crop residues, left on the soil surface in the NT, and mechanically broken and incorporated into the soil in the CT. Differences between the CS and the CR were slighter, 10% higher for the CS, but not statistically different. Most of the sequences belonged to fungi (Verticillium, and Colletotrichum for lipases and laccases, and Aspergillus for proteases), and to the archaea Sulfolobus acidocaldarius for amylases. Our results indicate that agricultural soils under conservative managements may represent a hotspotfor bioprospection of hydrolases.
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2018-12-06
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Subjects: | Metagenome, Manejo do Solo, Plantio Direto, Microbiologia do Solo, Soil management, Soil enzymes, No-tillage, Metagenomics, Microbiome, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100838 |
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dig-alice-doc-11008382018-12-06T23:33:30Z Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach. SOUZA, R. C. CANTAO, M. E. NOGUEIRA, M. A. VASCONCELOS, A. T. R. HUNGRIA, M. RENATA CAROLINI SOUZA, CNPq; MAURICIO EGIDIO CANTAO, CNPSA; MARCO ANTONIO NOGUEIRA, CNPSO; ANA TEREZA RIBEIRO VASCONCELOS, CNPq; MARIANGELA HUNGRIA DA CUNHA, CNPSO. Metagenome Manejo do Solo Plantio Direto Microbiologia do Solo Soil management Soil enzymes No-tillage Metagenomics Microbiome Abstract: The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the tillage system, with the NT showing about five times more hydrolases than the CT system. The outstanding differences can be attributed to the management of crop residues, left on the soil surface in the NT, and mechanically broken and incorporated into the soil in the CT. Differences between the CS and the CR were slighter, 10% higher for the CS, but not statistically different. Most of the sequences belonged to fungi (Verticillium, and Colletotrichum for lipases and laccases, and Aspergillus for proteases), and to the archaea Sulfolobus acidocaldarius for amylases. Our results indicate that agricultural soils under conservative managements may represent a hotspotfor bioprospection of hydrolases. 2018-12-06T23:33:24Z 2018-12-06T23:33:24Z 2018-12-06 2018 2018-12-10T11:11:11Z Artigo de periódico Brazilian Journal of Microbiology, v. 49, p. 723-730, 2018. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100838 10.1016/j.bjm.2018.03.001. en eng openAccess |
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Metagenome Manejo do Solo Plantio Direto Microbiologia do Solo Soil management Soil enzymes No-tillage Metagenomics Microbiome Metagenome Manejo do Solo Plantio Direto Microbiologia do Solo Soil management Soil enzymes No-tillage Metagenomics Microbiome |
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Metagenome Manejo do Solo Plantio Direto Microbiologia do Solo Soil management Soil enzymes No-tillage Metagenomics Microbiome Metagenome Manejo do Solo Plantio Direto Microbiologia do Solo Soil management Soil enzymes No-tillage Metagenomics Microbiome SOUZA, R. C. CANTAO, M. E. NOGUEIRA, M. A. VASCONCELOS, A. T. R. HUNGRIA, M. Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach. |
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Abstract: The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the tillage system, with the NT showing about five times more hydrolases than the CT system. The outstanding differences can be attributed to the management of crop residues, left on the soil surface in the NT, and mechanically broken and incorporated into the soil in the CT. Differences between the CS and the CR were slighter, 10% higher for the CS, but not statistically different. Most of the sequences belonged to fungi (Verticillium, and Colletotrichum for lipases and laccases, and Aspergillus for proteases), and to the archaea Sulfolobus acidocaldarius for amylases. Our results indicate that agricultural soils under conservative managements may represent a hotspotfor bioprospection of hydrolases. |
author2 |
RENATA CAROLINI SOUZA, CNPq; MAURICIO EGIDIO CANTAO, CNPSA; MARCO ANTONIO NOGUEIRA, CNPSO; ANA TEREZA RIBEIRO VASCONCELOS, CNPq; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
author_facet |
RENATA CAROLINI SOUZA, CNPq; MAURICIO EGIDIO CANTAO, CNPSA; MARCO ANTONIO NOGUEIRA, CNPSO; ANA TEREZA RIBEIRO VASCONCELOS, CNPq; MARIANGELA HUNGRIA DA CUNHA, CNPSO. SOUZA, R. C. CANTAO, M. E. NOGUEIRA, M. A. VASCONCELOS, A. T. R. HUNGRIA, M. |
format |
Artigo de periódico |
topic_facet |
Metagenome Manejo do Solo Plantio Direto Microbiologia do Solo Soil management Soil enzymes No-tillage Metagenomics Microbiome |
author |
SOUZA, R. C. CANTAO, M. E. NOGUEIRA, M. A. VASCONCELOS, A. T. R. HUNGRIA, M. |
author_sort |
SOUZA, R. C. |
title |
Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach. |
title_short |
Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach. |
title_full |
Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach. |
title_fullStr |
Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach. |
title_full_unstemmed |
Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach. |
title_sort |
outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach. |
publishDate |
2018-12-06 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100838 |
work_keys_str_mv |
AT souzarc outstandingimpactofsoiltillageontheabundanceofsoilhydrolasesrevealedbyametagenomicapproach AT cantaome outstandingimpactofsoiltillageontheabundanceofsoilhydrolasesrevealedbyametagenomicapproach AT nogueirama outstandingimpactofsoiltillageontheabundanceofsoilhydrolasesrevealedbyametagenomicapproach AT vasconcelosatr outstandingimpactofsoiltillageontheabundanceofsoilhydrolasesrevealedbyametagenomicapproach AT hungriam outstandingimpactofsoiltillageontheabundanceofsoilhydrolasesrevealedbyametagenomicapproach |
_version_ |
1756025368907612160 |