Exploiting sorghum genetic diversity for enhanced aluminum tolerance: allele mining based on the AltSB locus.
Root damage due to aluminum (Al) toxicity restricts crop production on acidic soils, which are extensive in the tropics. The sorghum root Al-activated citrate transporter, SbMATE, underlies the Al tolerance locus, AltSB, and increases grain yield under Al toxicity. Here, AltSB loci associated with Al tolerance were converted into Amplification Refractory Mutation System (ARMS) markers, which are cost effective and easy to use. A DNA pooling strategy allowed us to identify accessions harboring rare favorable AltSB alleles in three germplasm sets while greatly reducing genotyping needs. Population structure analysis revealed that favorable AltSB alleles are predominantly found in subpopulations enriched with guinea sorghums, supporting a possible Western African origin of AltSB. The efficiency of allele mining in recovering Al tolerance accessions was the highest in the largest and highly diverse germplasm set, with a 10-fold reduction in the number of accessions that would need to be phenotyped in the absence of marker information. Finally, Al tolerant accessions were found to rely on SbMATE to exclude Al3+ from sensitive sites in the root apex. This study emphasizes gene-specific markers as important tools for efficiently mining useful rare alleles in diverse germplasm, bridging genetic resource conservation efforts and pre-breeding for Al tolerance.
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Format: | Artigo de periódico biblioteca |
Language: | English eng |
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2018-07-24
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Subjects: | Tolerância, Toxicidade, Alumínio, Germoplasma, Marcador Genético, Sorgo, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1093494 |
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dig-alice-doc-10934942018-07-25T01:16:26Z Exploiting sorghum genetic diversity for enhanced aluminum tolerance: allele mining based on the AltSB locus. HUFNAGEL, B. GUIMARÃES, C. T. CRAFT, E. J. SHAFF, J. E. SCHAFFERT, R. E. KOCHIAN, L. V. MAGALHAES, J. V. Universidade Federal de Minas Gerais; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; Robert W. Holley Center of Agriculture & Health, Ithaca; Robert W. Holley Center of Agriculture & Health, Ithaca; ROBERT EUGENE SCHAFFERT, CNPMS; Global Institute for Food Security, University of Saskatchewan, Saskatoon.; JURANDIR VIEIRA DE MAGALHAES, CNPMS. Tolerância Toxicidade Alumínio Germoplasma Marcador Genético Sorgo Root damage due to aluminum (Al) toxicity restricts crop production on acidic soils, which are extensive in the tropics. The sorghum root Al-activated citrate transporter, SbMATE, underlies the Al tolerance locus, AltSB, and increases grain yield under Al toxicity. Here, AltSB loci associated with Al tolerance were converted into Amplification Refractory Mutation System (ARMS) markers, which are cost effective and easy to use. A DNA pooling strategy allowed us to identify accessions harboring rare favorable AltSB alleles in three germplasm sets while greatly reducing genotyping needs. Population structure analysis revealed that favorable AltSB alleles are predominantly found in subpopulations enriched with guinea sorghums, supporting a possible Western African origin of AltSB. The efficiency of allele mining in recovering Al tolerance accessions was the highest in the largest and highly diverse germplasm set, with a 10-fold reduction in the number of accessions that would need to be phenotyped in the absence of marker information. Finally, Al tolerant accessions were found to rely on SbMATE to exclude Al3+ from sensitive sites in the root apex. This study emphasizes gene-specific markers as important tools for efficiently mining useful rare alleles in diverse germplasm, bridging genetic resource conservation efforts and pre-breeding for Al tolerance. Article number: 10094. 2018-07-25T01:16:19Z 2018-07-25T01:16:19Z 2018-07-24 2018 2019-02-05T11:11:11Z Artigo de periódico Scientific Reports, v. 8, p. 1-13, July 2018. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1093494 10.1038/s41598-018-27817-z en eng openAccess |
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Tolerância Toxicidade Alumínio Germoplasma Marcador Genético Sorgo Tolerância Toxicidade Alumínio Germoplasma Marcador Genético Sorgo |
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Tolerância Toxicidade Alumínio Germoplasma Marcador Genético Sorgo Tolerância Toxicidade Alumínio Germoplasma Marcador Genético Sorgo HUFNAGEL, B. GUIMARÃES, C. T. CRAFT, E. J. SHAFF, J. E. SCHAFFERT, R. E. KOCHIAN, L. V. MAGALHAES, J. V. Exploiting sorghum genetic diversity for enhanced aluminum tolerance: allele mining based on the AltSB locus. |
description |
Root damage due to aluminum (Al) toxicity restricts crop production on acidic soils, which are extensive in the tropics. The sorghum root Al-activated citrate transporter, SbMATE, underlies the Al tolerance locus, AltSB, and increases grain yield under Al toxicity. Here, AltSB loci associated with Al tolerance were converted into Amplification Refractory Mutation System (ARMS) markers, which are cost effective and easy to use. A DNA pooling strategy allowed us to identify accessions harboring rare favorable AltSB alleles in three germplasm sets while greatly reducing genotyping needs. Population structure analysis revealed that favorable AltSB alleles are predominantly found in subpopulations enriched with guinea sorghums, supporting a possible Western African origin of AltSB. The efficiency of allele mining in recovering Al tolerance accessions was the highest in the largest and highly diverse germplasm set, with a 10-fold reduction in the number of accessions that would need to be phenotyped in the absence of marker information. Finally, Al tolerant accessions were found to rely on SbMATE to exclude Al3+ from sensitive sites in the root apex. This study emphasizes gene-specific markers as important tools for efficiently mining useful rare alleles in diverse germplasm, bridging genetic resource conservation efforts and pre-breeding for Al tolerance. |
author2 |
Universidade Federal de Minas Gerais; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; Robert W. Holley Center of Agriculture & Health, Ithaca; Robert W. Holley Center of Agriculture & Health, Ithaca; ROBERT EUGENE SCHAFFERT, CNPMS; Global Institute for Food Security, University of Saskatchewan, Saskatoon.; JURANDIR VIEIRA DE MAGALHAES, CNPMS. |
author_facet |
Universidade Federal de Minas Gerais; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; Robert W. Holley Center of Agriculture & Health, Ithaca; Robert W. Holley Center of Agriculture & Health, Ithaca; ROBERT EUGENE SCHAFFERT, CNPMS; Global Institute for Food Security, University of Saskatchewan, Saskatoon.; JURANDIR VIEIRA DE MAGALHAES, CNPMS. HUFNAGEL, B. GUIMARÃES, C. T. CRAFT, E. J. SHAFF, J. E. SCHAFFERT, R. E. KOCHIAN, L. V. MAGALHAES, J. V. |
format |
Artigo de periódico |
topic_facet |
Tolerância Toxicidade Alumínio Germoplasma Marcador Genético Sorgo |
author |
HUFNAGEL, B. GUIMARÃES, C. T. CRAFT, E. J. SHAFF, J. E. SCHAFFERT, R. E. KOCHIAN, L. V. MAGALHAES, J. V. |
author_sort |
HUFNAGEL, B. |
title |
Exploiting sorghum genetic diversity for enhanced aluminum tolerance: allele mining based on the AltSB locus. |
title_short |
Exploiting sorghum genetic diversity for enhanced aluminum tolerance: allele mining based on the AltSB locus. |
title_full |
Exploiting sorghum genetic diversity for enhanced aluminum tolerance: allele mining based on the AltSB locus. |
title_fullStr |
Exploiting sorghum genetic diversity for enhanced aluminum tolerance: allele mining based on the AltSB locus. |
title_full_unstemmed |
Exploiting sorghum genetic diversity for enhanced aluminum tolerance: allele mining based on the AltSB locus. |
title_sort |
exploiting sorghum genetic diversity for enhanced aluminum tolerance: allele mining based on the altsb locus. |
publishDate |
2018-07-24 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1093494 |
work_keys_str_mv |
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