Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.

The objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility.

Bibliographic Details

Main Authors:	GONÇALVES, A. N. D., SOARES, C. O., SANCHES, S. C., REIS, F. A., ROSINHA, G. M. S.
Other Authors:	ALINE NAJARA DOMINGOS GONÇALVES, Universidade Federal de Mato Grosso do sul; CLEBER OLIVEIRA SOARES, CNPGC; SIMONE CAMARGO SANCHES, Universidade Federal de Mato Grosso do Sul; FERNANDO ALVARENGA REIS, CNPC; GRACIA MARIA SOARES ROSINHA, CNPGC.
Format:	Artigo de periódico biblioteca
Language:	pt_BR por
Published:	2016-08-09
Subjects:	Encefalopatia espongiforme transmissivel, Gene PRNP, Genotipagem, Polimorfismo de nucleotídeo único, Single nucleotide polymorphisms, Transmissible spongiform encephalopathies., genotyping, prion diseases.,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1050554
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