Cytogenetic characterization of the Passiflora edulis Sims x Passiflora cincinnata Mast. interspecific hybrid and its parents.

The genus Passiflora L. consists of approximately 530 widely distributed species, including Passiflora edulis, which has drawn interest because of its commercial and agronomic value. Passiflora cincinnata is another important species owing to its long flowering period and resistance or tolerance to diseases and pests. In the present study, the meiotic segregation and pollen viability of an interspecific hybrid (P. edulis x P. cincinnata) and its parents were analyzed. The genomic contents were characterized using chromomycin A3 (CMA3)/40-60-diamidino-2-phenylindole (DAPI) staining, fluorescent in situ hybridization with 5S/45S ribosomal DNA (rDNA), genomic in situ hybridization (GISH), and inter-simple sequence repeat (ISSR) markers. The results indicated the diploid chromosome number for the parents and interspecific hybrid was 2n = 18. We also observed regular meiosis, one pair of S rDNA sites, and two pairs of 45S rDNA sites that colocalized with two pairs of CMA3 /DAPI- bands. The GISH data revealed three distinct chromosomal groups in the hybrid. The genetic origins of the interspecific hybrid, and its relationship with its parents were also confirmed using ISSR markers.

Bibliographic Details

Main Authors:	COELHO, M. do S. E., BORTOLETI, K. C. de A., ARAUJO, F. P. de, MELO, N. F. de
Other Authors:	MARIA DO SOCORRO EVANGELISTA COELHO, Universidade Estadual de Feira de Santana; KYRIA CILENE DE ANDRADE BORTOLETI, Universidade Federal do Vale do São Francisco; FRANCISCO PINHEIRO DE ARAUJO, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA.
Format:	Artigo de periódico biblioteca
Language:	English eng
Published:	2016-07-08
Subjects:	Passiflora cincinnata, Melhoramento genético, Passion fruit., Maracujá, Genótipo, Passiflora Edulis.,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1048475
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