Monoallelic expression of NNAT gene in Nelore steers skeletal muscle.

ABSTRACT: The NNAT gene plays a role on adipogenesis, brain development and insulin secretion. NNAT is described as paternal imprinted in bovine fetal tissue, but it is still underexplored in adult tissues. In order to improve the understanding of NNAT expression, muscle from Nellore was used to assess its allelic expression. Genomic DNA from 38 steers was genotyped for identification of heterozygous individuals with known allele origin. Total mRNA from muscle was extracted and sequenced by HiScanSQ, and afterwards the reads of each allele were counted for each animal. A total of 8 reciprocal heterozygous had more than 10 reads for the chosen SNP. All animals showed monoallelic expression, being 5 with paternal allele expressed, while 7 had the G allele expressed. These results demonstrate that NNAT may not have the paternal imprint conserved in adult bovine muscle, and it suggests a G allele-dependent expression.

Bibliographic Details

Main Authors:	SOUZA, M. M., ZERLOTINI, A., TIZIOTO, P. C., OLIVEIRA, P. S. N., SOMAVILLA, A. L., MOKRY, F. B., CESAR, A. S. M., DINIZ, W. J. S., MUDADU, M. A., NICIURA, S. C. M., COUTINHO, L. L., REGITANO, L. C. A.
Other Authors:	UFSCar; ADHEMAR ZERLOTINI NETO, CNPTIA; UFSCar; UFSCar; Unesp Jaboticabal; UFSCar; Universidade de São Paulo, Piracicaba; UFSCar; MAURICIO DE ALVARENGA MUDADU, CPPSE; SIMONE CRISTINA MEO NICIURA, CPPSE; Universidade de São Paulo, Piracicaba; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
Format:	Anais e Proceedings de eventos biblioteca
Language:	English eng
Published:	2015-01-20
Subjects:	Neuronatin, Zebu, Genomic imprinting,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1006155
Tags:	Add Tag No Tags, Be the first to tag this record!