Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought.

Drought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α > β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4α genes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice.

Bibliographic Details

Main Authors:	BEVITORI, R., OLIVEIRA, M. B., GROSSI-DE-SÁ, M. F., LANNA, A. C., SILVEIRA, R. D. da, PETROFEZA, S.
Other Authors:	ROSANGELA BEVITORI, CNPAF; MARILIA BARROS OLIVEIRA, doutoranda UFG; MARIA FATIMA GROSSI DE SA, CENARGEN; ANNA CRISTINA LANNA, CNPAF; RICARDO DIÓGENES DA SILVEIRA; SILVANA PETROFEZA, UFG.
Format:	Artigo de periódico biblioteca
Language:	English eng
Published:	2014-12-03
Subjects:	Arroz, Oryza sativa, Doença de planta, Brusone, Genética molecular, Seca, Gene expression, Rice, Magnaporthe oryzae, Blast disease,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1001427 http://dx.doi.org/10.4238/2014.November.27.7
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