Uncovering species boundaries in the Neotropical ant complex Ectatomma ruidum (Ectatomminae) under the presence of nuclear mitochondrial paralogues
Nuclear mitochondrial (mt) paralogues (numts) are non-functional fragments of mtDNA integrated into the nuclear genome that can overestimate the number of species in analyses based on mtDNA sequences. As numts have relatively slow mutation rates, they can pass undetected by conventional procedures such as inspecting for internal stop codons, indels or apparent polymorphism in chromatograms. Species boundaries based on mtDNA markers therefore require a thorough assessment of numts, especially in insects, where this phenomenon appears to be relatively frequent. Ectatomma ruidum is a widely distributed Neotropical ant species that is distributed from northern Mexico to northern Brazil. Previous behavioural and molecular evidence suggests that this species actually represents a composite taxon. Here we assessed the species boundaries in E. ruidum based on two mt (COI, cyt b) and one nuclear (H3) marker, as well as on external morphology. Ancient and recent mt paralogues were detected in several specimens, although pre-PCR dilution of DNA template helped to recover most of the mt orthologues. Based on the congruence found between our species delineation obtained from the mt genealogies and the discriminated morphospecies, we propose that E. ruidum is actually composed of at least three species. Two of these species have a wide geographical distribution in the Neotropics, whereas the remaining one was restricted to localities situated near the Pacific coast in south-east Mexico. We also found extensive intra- and interspecific variation in the barcoding locus. Moreover, the nuclear evidence suggests the existence of hybrids between two of these species in Oaxaca, south-east Mexico. This study agrees with previous studies of other closely related animal taxa, which have revealed a complex evolutionary history and overlooked species diversity in the latter region.
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Format: | Texto biblioteca |
Language: | eng |
Subjects: | Ectatomma ruidum, Hibridación, Taxonomía animal, Ácido desoxirribonucléico, Zoogeografía, |
Online Access: | http://onlinelibrary.wiley.com/doi/10.1111/zoj.12407/abstract |
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Ectatomma ruidum Hibridación Taxonomía animal Ácido desoxirribonucléico Zoogeografía Ectatomma ruidum Hibridación Taxonomía animal Ácido desoxirribonucléico Zoogeografía |
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Ectatomma ruidum Hibridación Taxonomía animal Ácido desoxirribonucléico Zoogeografía Ectatomma ruidum Hibridación Taxonomía animal Ácido desoxirribonucléico Zoogeografía Aguilar Velasco, Reina Gabriela Poteaux, Chantal autor/a 22572 Meza Lázaro, Rubí autor/a Lachaud, Jean Paul Doctor autor/a 2090 Dubovikoff, Dmitry A. autor/a 13272 Zaldívar Riverón, Alejandro autor/a Uncovering species boundaries in the Neotropical ant complex Ectatomma ruidum (Ectatomminae) under the presence of nuclear mitochondrial paralogues |
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Nuclear mitochondrial (mt) paralogues (numts) are non-functional fragments of mtDNA integrated into the nuclear genome that can overestimate the number of species in analyses based on mtDNA sequences. As numts have relatively slow mutation rates, they can pass undetected by conventional procedures such as inspecting for internal stop codons, indels or apparent polymorphism in chromatograms. Species boundaries based on mtDNA markers therefore require a thorough assessment of numts, especially in insects, where this phenomenon appears to be relatively frequent. Ectatomma ruidum is a widely distributed Neotropical ant species that is distributed from northern Mexico to northern Brazil. Previous behavioural and molecular evidence suggests that this species actually represents a composite taxon. Here we assessed the species boundaries in E. ruidum based on two mt (COI, cyt b) and one nuclear (H3) marker, as well as on external morphology. Ancient and recent mt paralogues were detected in several specimens, although pre-PCR dilution of DNA template helped to recover most of the mt orthologues. Based on the congruence found between our species delineation obtained from the mt genealogies and the discriminated morphospecies, we propose that E. ruidum is actually composed of at least three species. Two of these species have a wide geographical distribution in the Neotropics, whereas the remaining one was restricted to localities situated near the Pacific coast in south-east Mexico. We also found extensive intra- and interspecific variation in the barcoding locus. Moreover, the nuclear evidence suggests the existence of hybrids between two of these species in Oaxaca, south-east Mexico. This study agrees with previous studies of other closely related animal taxa, which have revealed a complex evolutionary history and overlooked species diversity in the latter region. |
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Ectatomma ruidum Hibridación Taxonomía animal Ácido desoxirribonucléico Zoogeografía |
author |
Aguilar Velasco, Reina Gabriela Poteaux, Chantal autor/a 22572 Meza Lázaro, Rubí autor/a Lachaud, Jean Paul Doctor autor/a 2090 Dubovikoff, Dmitry A. autor/a 13272 Zaldívar Riverón, Alejandro autor/a |
author_facet |
Aguilar Velasco, Reina Gabriela Poteaux, Chantal autor/a 22572 Meza Lázaro, Rubí autor/a Lachaud, Jean Paul Doctor autor/a 2090 Dubovikoff, Dmitry A. autor/a 13272 Zaldívar Riverón, Alejandro autor/a |
author_sort |
Aguilar Velasco, Reina Gabriela |
title |
Uncovering species boundaries in the Neotropical ant complex Ectatomma ruidum (Ectatomminae) under the presence of nuclear mitochondrial paralogues |
title_short |
Uncovering species boundaries in the Neotropical ant complex Ectatomma ruidum (Ectatomminae) under the presence of nuclear mitochondrial paralogues |
title_full |
Uncovering species boundaries in the Neotropical ant complex Ectatomma ruidum (Ectatomminae) under the presence of nuclear mitochondrial paralogues |
title_fullStr |
Uncovering species boundaries in the Neotropical ant complex Ectatomma ruidum (Ectatomminae) under the presence of nuclear mitochondrial paralogues |
title_full_unstemmed |
Uncovering species boundaries in the Neotropical ant complex Ectatomma ruidum (Ectatomminae) under the presence of nuclear mitochondrial paralogues |
title_sort |
uncovering species boundaries in the neotropical ant complex ectatomma ruidum (ectatomminae) under the presence of nuclear mitochondrial paralogues |
url |
http://onlinelibrary.wiley.com/doi/10.1111/zoj.12407/abstract |
work_keys_str_mv |
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KOHA-OAI-ECOSUR:98042024-03-12T12:44:06ZUncovering species boundaries in the Neotropical ant complex Ectatomma ruidum (Ectatomminae) under the presence of nuclear mitochondrial paralogues Aguilar Velasco, Reina Gabriela Poteaux, Chantal autor/a 22572 Meza Lázaro, Rubí autor/a Lachaud, Jean Paul Doctor autor/a 2090 Dubovikoff, Dmitry A. autor/a 13272 Zaldívar Riverón, Alejandro autor/a textengNuclear mitochondrial (mt) paralogues (numts) are non-functional fragments of mtDNA integrated into the nuclear genome that can overestimate the number of species in analyses based on mtDNA sequences. As numts have relatively slow mutation rates, they can pass undetected by conventional procedures such as inspecting for internal stop codons, indels or apparent polymorphism in chromatograms. Species boundaries based on mtDNA markers therefore require a thorough assessment of numts, especially in insects, where this phenomenon appears to be relatively frequent. Ectatomma ruidum is a widely distributed Neotropical ant species that is distributed from northern Mexico to northern Brazil. Previous behavioural and molecular evidence suggests that this species actually represents a composite taxon. Here we assessed the species boundaries in E. ruidum based on two mt (COI, cyt b) and one nuclear (H3) marker, as well as on external morphology. Ancient and recent mt paralogues were detected in several specimens, although pre-PCR dilution of DNA template helped to recover most of the mt orthologues. Based on the congruence found between our species delineation obtained from the mt genealogies and the discriminated morphospecies, we propose that E. ruidum is actually composed of at least three species. Two of these species have a wide geographical distribution in the Neotropics, whereas the remaining one was restricted to localities situated near the Pacific coast in south-east Mexico. We also found extensive intra- and interspecific variation in the barcoding locus. Moreover, the nuclear evidence suggests the existence of hybrids between two of these species in Oaxaca, south-east Mexico. This study agrees with previous studies of other closely related animal taxa, which have revealed a complex evolutionary history and overlooked species diversity in the latter region.Nuclear mitochondrial (mt) paralogues (numts) are non-functional fragments of mtDNA integrated into the nuclear genome that can overestimate the number of species in analyses based on mtDNA sequences. As numts have relatively slow mutation rates, they can pass undetected by conventional procedures such as inspecting for internal stop codons, indels or apparent polymorphism in chromatograms. Species boundaries based on mtDNA markers therefore require a thorough assessment of numts, especially in insects, where this phenomenon appears to be relatively frequent. Ectatomma ruidum is a widely distributed Neotropical ant species that is distributed from northern Mexico to northern Brazil. Previous behavioural and molecular evidence suggests that this species actually represents a composite taxon. Here we assessed the species boundaries in E. ruidum based on two mt (COI, cyt b) and one nuclear (H3) marker, as well as on external morphology. Ancient and recent mt paralogues were detected in several specimens, although pre-PCR dilution of DNA template helped to recover most of the mt orthologues. Based on the congruence found between our species delineation obtained from the mt genealogies and the discriminated morphospecies, we propose that E. ruidum is actually composed of at least three species. Two of these species have a wide geographical distribution in the Neotropics, whereas the remaining one was restricted to localities situated near the Pacific coast in south-east Mexico. We also found extensive intra- and interspecific variation in the barcoding locus. Moreover, the nuclear evidence suggests the existence of hybrids between two of these species in Oaxaca, south-east Mexico. This study agrees with previous studies of other closely related animal taxa, which have revealed a complex evolutionary history and overlooked species diversity in the latter region.Adobe Acrobat profesional 6.0 o superiorEctatomma ruidumHibridaciónTaxonomía animalÁcido desoxirribonucléicoZoogeografíaDisponible en líneaZoological Journal of the Linnean Societyhttp://onlinelibrary.wiley.com/doi/10.1111/zoj.12407/abstractAcceso en línea sin restricciones |