Microplate assay analysis of the distribution of organophosphate and carbamate resistance in Guatemalan Anopheles albimanus
Simple microplate assay methods for determining the frequency of insecticide resistance in single mosquitos were used to study the distribution and localization of organophosphate and carbamate resistance in field populations of Anopheles albimanus Weidemann in Guatemala, where such resistance, caused by heavy use of agricultural pesticides, has long been assumed to be widespread. Areas of complete susceptibility to organophophates and carbamates were observed, as well as areas where the resistant phenotypes represented up to 98 percent of the population. Overall, the resistance levels were lower and more localized than expected. Two mechanisms of resistance were identified by the microassay methods. These were the elevated esterase (nonspecific esterase) and insensitive acetylcholinesterase mechanisms which were selected independently, the former (documented for the first time in Central American anophelines) being predominant. These methods represent a promising new technology for the detection and assessment of resistance and will facilitate improved control strategy decisions.
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Subjects: | Insecticidas organofosforados, Resistencia a los plaguicidas, Anopheles albimanus, Carbamatos, Control de plagas, Artfrosur, |
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KOHA-OAI-ECOSUR:404652022-08-18T14:29:06ZMicroplate assay analysis of the distribution of organophosphate and carbamate resistance in Guatemalan Anopheles albimanus Brogdon, W. G. Beach, R. F. coaut. Stewart, J. M. autor/a Castanaza, L. autor/a textengSimple microplate assay methods for determining the frequency of insecticide resistance in single mosquitos were used to study the distribution and localization of organophosphate and carbamate resistance in field populations of Anopheles albimanus Weidemann in Guatemala, where such resistance, caused by heavy use of agricultural pesticides, has long been assumed to be widespread. Areas of complete susceptibility to organophophates and carbamates were observed, as well as areas where the resistant phenotypes represented up to 98 percent of the population. Overall, the resistance levels were lower and more localized than expected. Two mechanisms of resistance were identified by the microassay methods. These were the elevated esterase (nonspecific esterase) and insensitive acetylcholinesterase mechanisms which were selected independently, the former (documented for the first time in Central American anophelines) being predominant. These methods represent a promising new technology for the detection and assessment of resistance and will facilitate improved control strategy decisions.Simple microplate assay methods for determining the frequency of insecticide resistance in single mosquitos were used to study the distribution and localization of organophosphate and carbamate resistance in field populations of Anopheles albimanus Weidemann in Guatemala, where such resistance, caused by heavy use of agricultural pesticides, has long been assumed to be widespread. Areas of complete susceptibility to organophophates and carbamates were observed, as well as areas where the resistant phenotypes represented up to 98 percent of the population. Overall, the resistance levels were lower and more localized than expected. Two mechanisms of resistance were identified by the microassay methods. These were the elevated esterase (nonspecific esterase) and insensitive acetylcholinesterase mechanisms which were selected independently, the former (documented for the first time in Central American anophelines) being predominant. These methods represent a promising new technology for the detection and assessment of resistance and will facilitate improved control strategy decisions.Insecticidas organofosforadosResistencia a los plaguicidasAnopheles albimanusCarbamatosControl de plagasArtfrosurBulletin of the World Health Organization |
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Insecticidas organofosforados Resistencia a los plaguicidas Anopheles albimanus Carbamatos Control de plagas Artfrosur Insecticidas organofosforados Resistencia a los plaguicidas Anopheles albimanus Carbamatos Control de plagas Artfrosur |
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Insecticidas organofosforados Resistencia a los plaguicidas Anopheles albimanus Carbamatos Control de plagas Artfrosur Insecticidas organofosforados Resistencia a los plaguicidas Anopheles albimanus Carbamatos Control de plagas Artfrosur Brogdon, W. G. Beach, R. F. coaut. Stewart, J. M. autor/a Castanaza, L. autor/a Microplate assay analysis of the distribution of organophosphate and carbamate resistance in Guatemalan Anopheles albimanus |
description |
Simple microplate assay methods for determining the frequency of insecticide resistance in single mosquitos were used to study the distribution and localization of organophosphate and carbamate resistance in field populations of Anopheles albimanus Weidemann in Guatemala, where such resistance, caused by heavy use of agricultural pesticides, has long been assumed to be widespread. Areas of complete susceptibility to organophophates and carbamates were observed, as well as areas where the resistant phenotypes represented up to 98 percent of the population. Overall, the resistance levels were lower and more localized than expected. Two mechanisms of resistance were identified by the microassay methods. These were the elevated esterase (nonspecific esterase) and insensitive acetylcholinesterase mechanisms which were selected independently, the former (documented for the first time in Central American anophelines) being predominant. These methods represent a promising new technology for the detection and assessment of resistance and will facilitate improved control strategy decisions. |
format |
Texto |
topic_facet |
Insecticidas organofosforados Resistencia a los plaguicidas Anopheles albimanus Carbamatos Control de plagas Artfrosur |
author |
Brogdon, W. G. Beach, R. F. coaut. Stewart, J. M. autor/a Castanaza, L. autor/a |
author_facet |
Brogdon, W. G. Beach, R. F. coaut. Stewart, J. M. autor/a Castanaza, L. autor/a |
author_sort |
Brogdon, W. G. |
title |
Microplate assay analysis of the distribution of organophosphate and carbamate resistance in Guatemalan Anopheles albimanus |
title_short |
Microplate assay analysis of the distribution of organophosphate and carbamate resistance in Guatemalan Anopheles albimanus |
title_full |
Microplate assay analysis of the distribution of organophosphate and carbamate resistance in Guatemalan Anopheles albimanus |
title_fullStr |
Microplate assay analysis of the distribution of organophosphate and carbamate resistance in Guatemalan Anopheles albimanus |
title_full_unstemmed |
Microplate assay analysis of the distribution of organophosphate and carbamate resistance in Guatemalan Anopheles albimanus |
title_sort |
microplate assay analysis of the distribution of organophosphate and carbamate resistance in guatemalan anopheles albimanus |
work_keys_str_mv |
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