Formation of somatic embryos from protoplasts of Coffea arabica L.
Somatic embryos were regenerated from protoplasts isolated from embryogenic callus on young leaf explants from mature coffee trees. Embryos were regenerated on modified Murashige and Skoog medium supplemented with 5 uM BA. Somatic embryos developed into intact plants. Mannitol at 0.5 M was adequate as an osmoticum for isolating protoplasts, but subsequent culture required 0.3 M mannitol. A culture system in which osmolality was decreased gradually accelerated formation of colonies and somatic embryogenesis. Chemical name used: N-(phenylmethyl)-1H-purine-6-amine (BA).
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| Auteurs principaux: | , , , |
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| Format: | biblioteca |
| Publié: |
1994
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| Sujets: | COFFEA ARABICA, EMBRION SOMATICO, PROTOPLASTOS, CULTIVO DE CELULAS, PROPAGACION VEGETATIVA, CULTIVO DE TEJIDOS, MICROPROPAGACION, CITOCININAS, |
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| Résumé: | Somatic embryos were regenerated from protoplasts isolated from embryogenic callus on young leaf explants from mature coffee trees. Embryos were regenerated on modified Murashige and Skoog medium supplemented with 5 uM BA. Somatic embryos developed into intact plants. Mannitol at 0.5 M was adequate as an osmoticum for isolating protoplasts, but subsequent culture required 0.3 M mannitol. A culture system in which osmolality was decreased gradually accelerated formation of colonies and somatic embryogenesis. Chemical name used: N-(phenylmethyl)-1H-purine-6-amine (BA). |
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