Anther culture in crop plants

Cell and tissue culture techniques are no longer a monopoly of research laboratories: they have penetrated breeding institutes and private companies. Using haploids in potato, rape seed, wheat, and barley breeding programs is routine. Our results with these crops, especially those on resistance to viruses and fungi, are outlined. Particular emphasis is given to potato viruses and to barley yellow mosaic virus. Results from incorporating a haploid step into other important species are summarized. From isolated microspores in a liquid culture medium conditioned by ovaries, our regeneration rate of functional barley plants was high enough to use this system for selection. In our first attempt we applied fusaric acid (an unspecific toxin of several Fusarium species) as selective agent. Callus surviving on 0.2mM fusaric acid could be selected. In this system microspore culture combines the advantages of a single-cell system with those of haploidy. The influence of an unspecific selection during anther culture on the characters of androgenetic offspring is discussed

Bibliographic Details

Main Authors:	131047 Wenzel, G., 68524 Foroughi-Wehr, B., 83558 Kohler, F., 12955 IRRI, Manila (Filipinas), 35649 Inter-Center Seminar on International Agricultural Research Centers (IARCs) and Biotechnology Manila (Filipinas) 23-27 Abr 1984
Format:	Texto biblioteca
Language:	eng
Published:	Manila (Filipinas) 1985
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